Metabolism of the Perfused Cat Brain during Metrazol Convulsions and Electroshock

Nature ◽  
1958 ◽  
Vol 181 (4609) ◽  
pp. 626-627 ◽  
Author(s):  
C. ALLWEIS ◽  
J. MAGNES
2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S611-S611
Author(s):  
Nathalie Ginovart ◽  
Alan A Wilson ◽  
Matthaeus Willeit ◽  
Ken Singh ◽  
Douglas Hussey ◽  
...  
Keyword(s):  

2009 ◽  
Vol 12 (3) ◽  
pp. 250-258 ◽  
Author(s):  
Jin Su Kim ◽  
Jae Sung Lee ◽  
Min-Hyun Park ◽  
Kyeong Min Kim ◽  
Seung-Ha Oh ◽  
...  

1994 ◽  
Vol 33 (4) ◽  
pp. 393-402 ◽  
Author(s):  
W.C. Wu ◽  
S.D. Wang ◽  
J.C. Liu ◽  
H.T. Horng ◽  
M.J. Wayner ◽  
...  
Keyword(s):  

1993 ◽  
Vol 41 (4) ◽  
pp. 609-616 ◽  
Author(s):  
B M Riederer ◽  
R Porchet ◽  
R A Marugg ◽  
L I Binder

For accurate and quantitative immunohistochemical localization of antigens it is crucial to know the solubility of tissue proteins and their degree of loss during processing. In this study we focused on the solubility of several cytoskeletal proteins in cat brain tissue at various ages and their loss during immunohistochemical procedures. We further examined whether fixation affected either solubility or immunocytochemical detectability of several cytoskeletal proteins. An assay was designed to measure the solubility of cytoskeletal proteins in cryostat sections. Quantity and quality of proteins lost or remaining in tissue were measured and analyzed by electrophoresis and immunoblots. Most microtubule proteins were found to be soluble in unfixed and alcohol fixed tissues. Furthermore, the microtubule proteins remaining in the tissue had a changed cellular distribution. In contrast, brain spectrin and all three neurofilament subunits were insoluble and remained in the tissue, allowing their immunocytochemical localization in alcohol-fixed tissue. Synapsin I, a protein associated with the spectrin cytoskeleton, was soluble, and aldehyde fixation is advised for its immunohistochemical localization. With aldehyde fixation, the immunoreactivity of some antibodies against neurofilament proteins was reduced in axons unveiling novel immunogenic sites in nuclei that may represent artifacts of fixation. In conclusion, protein solubility and the effects of fixation are influential factors in cytoskeletal immunohistochemistry, and should be considered before assessments for a quantitative distribution are made.


1981 ◽  
Vol 18 (1-2) ◽  
pp. 72-79 ◽  
Author(s):  
William G. Webster
Keyword(s):  

1989 ◽  
Vol 9 (6) ◽  
pp. 886-891 ◽  
Author(s):  
David Barranco ◽  
Leslie N. Sutton ◽  
Sandra Florin ◽  
Joel Greenberg ◽  
Teresa Sinnwell ◽  
...  

19F NMR was used to determine washout curves of an inert, diffusible gas (CHF3) from the cat brain. The cerebral blood flow was estimated from a bi- or tri-phasic fit to the deconvoluted wash-out curve, using the Kety-Schmidt approach. Cerebral blood flow values determined by 19F NMR show the expected responsiveness to alterations in Paco2, but are approximately 28% lower than cerebral blood flow values determined simultaneously by radioactive microsphere techniques. High concentrations of CHF3 have little effect on intracranial pressure, mean arterial blood pressure or Paco2, but cause small changes in the blood flow to certain regions of the brain. We conclude that 19F NMR techniques utilizing low concentrations of CHF3 have potential for the noninvasive measurement of cerebral blood flow.


2006 ◽  
Vol 31 (3) ◽  
pp. 226-232 ◽  
Author(s):  
Nicolas Aznavour ◽  
Latifa Rbah ◽  
Lucienne Léger ◽  
Colette Buda ◽  
Jean-Pierre Sastre ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document