Molecular Weight of Ovalbumin and of Bovine Serum Albumin in Urea Solution

Nature ◽  
1955 ◽  
Vol 176 (4485) ◽  
pp. 738-738 ◽  
Author(s):  
H. A. McKENZIE ◽  
M. B. SMITH ◽  
R. G. WAKE
Keyword(s):  
Molecular Weight ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Urea Solution

The production of polyclonal antibodies against the mycotoxin derivative patulin hemiglutarate

1993 ◽  
Vol 39 (9) ◽  
pp. 861-863 ◽  
Author(s):  
L. J. McElroy ◽  
C. M. Weiss
Keyword(s):  
Molecular Weight ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Antibody Titer ◽  
Bovine Serum ◽  
Polyclonal Antibodies ◽  
Competitive Elisa ◽  
Low Molecular Weight ◽  
Specific Antibodies ◽  
Indirect Competitive Elisa

The mycotoxin patulin is a toxic, carcinogenic, unsaturated lactone produced by a number of molds. Polyclonal antibodies against patulin hemiglutarate were produced. Specific antibodies against patulin alone, however, were not clearly demonstrated. Because of its low molecular weight, patulin required conjugation to bovine serum albumin (BSA) to increase its immunogenicity. Anti-patulin-hemiglutarate-BSA antibody titer and specificity were determined using indirect and indirect competitive ELISA, respectively. Immunoassays would facilitate detection and quantitation of patulin.Key words: patulin, mycotoxin, antibodies.

A novel potentiometric sensor based on urease/ bovine serum albumin-poly(3,4-ethylenedioxythiophene)/Pt for urea detection

2016 ◽  
Vol 71 (4) ◽  
pp. 277-282 ◽  
Author(s):  
Chien-Hsing Hsu ◽  
Ya-Wei Hsu ◽  
Yu-Ching Weng
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
High Selectivity ◽  
Ph Value ◽  
Detection Sensitivity ◽  
Urea Solution ◽  
Pt Electrode ◽  
Amide Bonds ◽  
Order Of Magnitude

AbstractWe have presented a potentiometric urea sensor using an urease/bovine serum albumin (BSA)-poly(3,4-ethylenedioxythiophene)(PEDOT)/Pt electrode. A urea detection sensitivity of 15.2 mV/decade (order of magnitude) has been achieved. BSA trapped in the PEDOT matrix was employed to bond urease molecules on the surface of a BSA-PEDOT/Pt electrode via amide bonds formed between the carboxyl functional groups on the enzyme and the amines on the BSA. The effects of PEDOT thickness, pH value of the urea solution, urease concentration, and temperature on the urea detection sensitivity were also studied. The lifetime of the sensor was studied for a period of 10 weeks, and the average sensing degradation rate was about 9 % per week. This sensor displayed a high selectivity to urea over glucose, KCl, and NaCl.

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