scholarly journals An Antagonist of the Sterility Hormone Rutin in the Green Alga Chlamydomonas eugametos: Ombuoside = 7.4′-Dimethylrutin

Nature ◽  
1954 ◽  
Vol 173 (4396) ◽  
pp. 218-218 ◽  
Author(s):  
FRANZ MOEWUS ◽  
VENANCIO DEULOFEU
1985 ◽  
Vol 79 (3) ◽  
pp. 740-745 ◽  
Author(s):  
Frans M. Klis ◽  
Marieke R. Samson ◽  
Egbert Touw ◽  
Alan Musgrave ◽  
Herman van den Ende

Planta ◽  
1987 ◽  
Vol 170 (3) ◽  
pp. 314-321 ◽  
Author(s):  
Marieke R. Samson ◽  
Frans M. Klis ◽  
Wieger L. Homan ◽  
Piet van Egmond ◽  
Alan Musgrave ◽  
...  

1992 ◽  
Vol 102 (3) ◽  
pp. 469-474
Author(s):  
V. ZACHLEDER ◽  
H. VAN DEN ENDE

A procedure for routine synchronization of large amounts of the unicellular green alga Chlamydomonas eugametos in liquid culture by alternating light and dark periods is described. The synchronized populations were grown at various light intensities and temperatures. The effect of these variables on the lengths of parts of the cell cycle and the number of daughter cells per cell division was followed. The cell cycle of C. eugametos started with a period in which the cells increased in size only (precommitment period). The length of this period was dependent on both the light intensity and the temperature. At the end of this period, a key point of the cell cycle (called commitment point) was attained. From this point, the cell were committed to divide and cell reproduction was triggered. The following period (post-commitment period), during which daughter cells were formed, could be traversed without supply of external energy, and without further growth of the cells. However, if sufficient energy was supplied during this period, the cells were able to attain more commitment points, leading to a higher number of daughter cells. The postcommitment period was fairly constant within a certain range of light intensity. At light intensities leading to more commitment points, however, this period was prolonged. No evidence was found for circadian rhythms or endogenous factors of “Zeitgeber” type playing a role in the control of growth and reproductive sequences in the cell cycle of C. eugametos.


1994 ◽  
Vol 35 (2) ◽  
pp. 349-352
Author(s):  
Hans Klerk ◽  
John A.J. Van Himbergen ◽  
Alan Musgrave ◽  
Peter J.M. Van Haaster ◽  
Herman Van Den Ende

1969 ◽  
Vol 14 (2) ◽  
pp. 121-126 ◽  
Author(s):  
A. C. McBride ◽  
C. S. Gowans

Evidence is presented which indicates that the phenylalanine analog 2-amino-3-phenylbutanoic acid (APBA) induces mutation to APBA, streptomycin, and neamine resistance in the green alga Chlamydomonas eugametos. Apparent chromosome abnormalities (reciprocal translocations and inversions) were also found among some clones recovered after APBA treatment. The two, mutation and chromosome aberration, are separable.


1974 ◽  
Vol 52 (4) ◽  
pp. 715-717 ◽  
Author(s):  
K. Nakamura ◽  
W. L. Hepler ◽  
E. J. Shaskin ◽  
D. W. Brooks

The effect of chloroplatinic acid on the growth of the unicellular green alga Chlamydomonas eugametos has been studied. This platinum compound reduces the rate of cell division and induces palmelloid formation by preventing the liberation of dividing cells. Recovery from the chloroplatinic-acid effect occurs after removal of the compound from the medium.


Weed Science ◽  
1980 ◽  
Vol 28 (5) ◽  
pp. 515-520 ◽  
Author(s):  
F. D. Hess

The single-celled green algaChlamydomonas eugametoswas found to be well suited for detecting growth inhibitor activity of chemicals. Tests consisted of solubilizing technical grade compounds in 20-ml aliquots of a 1 × 105cells/ml zoospore culture. Commercially available growth inhibitor herbicides significantly inhibited synchronous cell population increases within 48 h at concentrations ranging from 1 × 10−4to 1 × 10−7M. Examples of compounds that inhibitedChlamydomonasmore than 50% were butylate (S-ethyl diisobutylthiocarbamate) and diallate [S-(2,3-dichloroallyl)diisopropylthiocarbamate] at 1 × 10−4M, CDEC [2 chloroallyl diethyldithiocarbamate] and propham (isopropyl carbanilate) at 1 × 10−5M, alachlor [2-chloro-2′,6′-diethyl-N-(methoxymethyl)acetanilide] and trifluralin [α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine] at 1 × 10−6M, and bifenox [methyl 5-(2,4-dichlorophenoxy)-2-nitrobenzoate] at 1 × 10−7M. Ethanol, dimethylsulfoxide (DMSO), or acetone can be used to solubilize herbicides in the aqueous medium. DMSO and ethanol are not detrimental to the cells if the concentration is kept at 1% v/v or less.


1985 ◽  
Vol 63 (5) ◽  
pp. 909-915 ◽  
Author(s):  
Kazuo Nakamura ◽  
Charles F. Landry ◽  
Christine A. Goertzen ◽  
N. Wayne Ikebuchi

To address the problem of amino acid auxotroph scarcities in algae, an explanation was sought specifically for the nonrecoverability of arginine auxotrophs in the unicellular green alga Chlamydomonas eugametos. Chlamydomonas reinhardtii, in which the auxotroph has been recovered, was taken as a reference. In C. eugametos, unlike previously reported in C. reinhardtii, the use of selective media free of [Formula: see text] appeared not to affect the mutation spectrum. Arginine supported growth as the sole nitrogen source and canavanine sulfate inhibited growth, but both effects were less pronounced in C. eugametos. The concentration ratios (based on intracellular arginine accumulation) in C. eugametos remained less than 23% that of C. reinhardtii. The results indicate that nonrecoverability of arginine auxotrophs in C. eugametos is due to insufficient cellular accessibility to arginine.


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