Outgrowths from Pieces of Helix aspersa, the Common Snail

Nature ◽  
1931 ◽  
Vol 128 (3241) ◽  
pp. 1002-1003 ◽  
Author(s):  
J. BRONTË GATENBY
1965 ◽  
Vol s3-106 (73) ◽  
pp. 3-13
Author(s):  
JOHN R. BAKER ◽  
ELIZABETH G. M. WILLIAMS

The cation of methyl green carriea two poaitive charges, that of malachite green only one; but the two dyes behave towards tissue-constituents in almost exactly the same way. These dyes are not specific for chromatin. They colour certain objects that are devoid of DNA, even when they are used in very dilute solution. The granules of cells called Körnchenzellen in the connective tissue of the common snail, Helix aspersa, are strongly coloured by both dyes from very dilute solutions, and thus provide a striking instance of the unspecificity of these dyes. Malachite green, which is stable and free from contamination by metachromstic impurities, can advantageously replace the methyl green commonly used in mixtures with pyronine. It is suggested that pyronine may have a greater capacity for penetrating into close-textured objects, such ss nucleoli and ribosomes, than methyl and malachite greens.


1982 ◽  
Vol 10 (5) ◽  
pp. 384-385 ◽  
Author(s):  
MARION E. WILLIAMSON ◽  
PIERS C. EMSON

1994 ◽  
Vol 24 (6) ◽  
pp. 507-512 ◽  
Author(s):  
Yu. V. Burov ◽  
E. I. Drozdova ◽  
A. S. Pivovarov ◽  
T. N. Robakidze
Keyword(s):  

Nature ◽  
1879 ◽  
Vol 20 (511) ◽  
pp. 363-363 ◽  
Author(s):  
JAMES WARD
Keyword(s):  

1981 ◽  
Vol 194 (2) ◽  
pp. 507-511 ◽  
Author(s):  
B Howard ◽  
P C H Mitchell ◽  
A Ritchie ◽  
K Simkiss ◽  
M Taylor

Certain cells in the hepatopancreas of the common garden snail (Helix aspersa) contain intracellular granules that are sites of metal-ion accumulation. These granules have been extracted and investigated by u.v. and i.r. spectroscopy, atomic-absorption spectroscopy, X-ray microanalysis, thermogravimetric analysis, enzymic assay and microanalysis. The deposits contain about 18% (w/w) water, 5% (w/w) organic matter and 76% (w/w) inorganic material of which the main components are Ca2+, Mg2+ and P2O7(4)-. The possible origin of these granules is discussed, as is their role in detoxifying heavy-metal ions.


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