Selective Interruption of Molecular Oscillation

ARTHUR FAIRBOURNE

doi:10.1038/111149a0

Isolation and Analysis of Six timeless Alleles That Cause Short- or Long-Period Circadian Rhythms in Drosophila

Genetics ◽

10.1093/genetics/156.2.665 ◽

2000 ◽

Vol 156 (2) ◽

pp. 665-675

Author(s):

Adrian Rothenfluh ◽

Marla Abodeely ◽

Jeffrey L Price ◽

Michael W Young

Keyword(s):

Nuclear Translocation ◽

Phase Response Curve ◽

Fixed Number ◽

Constant Darkness ◽

Genetic Screens ◽

Protein Levels ◽

Long Period ◽

Short Period ◽

Molecular Oscillation ◽

New Alleles

Abstract In genetic screens for Drosophila mutations affecting circadian locomotion rhythms, we have isolated six new alleles of the timeless (tim) gene. Two of these mutations cause short-period rhythms of 21–22 hr in constant darkness, and four result in long-period cycles of 26–28 hr. All alleles are semidominant. Studies of the genetic interactions of some of the tim alleles with period-altering period (per) mutations indicate that these interactions are close to multiplicative; a given allele changes the period length of the genetic background by a fixed percentage, rather than by a fixed number of hours. The timL1 allele was studied in molecular detail. The long behavioral period of timL1 is reflected in a lengthened molecular oscillation of per and tim RNA and protein levels. The lengthened period is partly caused by delayed nuclear translocation of TIML1 protein, shown directly by immunocytochemistry and indirectly by an analysis of the phase response curve of timL1 flies.

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Emerging of stretched and contracted helices of substituted polyacetylenes and its molecular oscillation in solution

Industrial Chemistry ◽

10.4172/2469-9764-c1-004 ◽

2017 ◽

Vol 03 (02) ◽

Author(s):

Masayoshi Tabata

Keyword(s):

Molecular Oscillation

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Molecular Oscillation Behind the Clockface

The Journal of Biochemistry ◽

10.1093/jb/mvg218 ◽

2003 ◽

Vol 134 (6) ◽

pp. 773-775 ◽

Cited By ~ 1

Author(s):

Y. Fukada

Keyword(s):

Molecular Oscillation

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Human Circadian Molecular Oscillation Development Using Induced Pluripotent Stem Cells

Journal of Biological Rhythms ◽

10.1177/0748730419865436 ◽

2019 ◽

Vol 34 (5) ◽

pp. 525-532 ◽

Cited By ~ 9

Author(s):

Yasuhiro Umemura ◽

Izumi Maki ◽

Yoshiki Tsuchiya ◽

Nobuya Koike ◽

Kazuhiro Yagita

Keyword(s):

Stem Cells ◽

Circadian Clock ◽

Induced Pluripotent Stem Cells ◽

Pluripotent Stem Cells ◽

Common Mechanism ◽

Mouse Development ◽

Human Ipscs ◽

Molecular Oscillation ◽

Induced Pluripotent ◽

Clock Protein

The mammalian circadian clock, which coordinates various physiological functions, develops gradually during ontogeny. Recently, we have reported the posttranscriptional suppression of CLOCK protein expression as a key mechanism of the emergence of the circadian clock during mouse development. However, whether a common mechanism regulates the development of the human circadian clock remains unclear. In the present study, we show that human induced pluripotent stem cells (iPSCs) have no discernible circadian molecular oscillation. In addition, in vitro differentiation culture of human iPSCs required a longer duration than that required in mouse for the emergence of circadian oscillations. The expression of CLOCK protein in undifferentiated human iPSCs was posttranscriptionally suppressed despite the expression of CLOCK mRNA, which is consistent with our previous observations in mouse embryonic stem cells, iPSCs, and early mouse embryos. These results suggest that CLOCK protein expressions could be posttranscriptionally suppressed in the early developmental stage not only in mice but also in humans.

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