Resistance to rice yellow mottle virus (RYMV) in cultivated African rice varieties containing RYMV transgenes

10.1038/10917 ◽  
1999 ◽  
Vol 17 (7) ◽  
pp. 702-707 ◽  
Author(s):  
Yvonne M. Pinto ◽  
Rosan A. Kok ◽  
David C. Baulcombe
Keyword(s):  
Mottle Virus ◽  
Rice Yellow Mottle Virus ◽  
Rice Varieties ◽  
African Rice ◽  
Yellow Mottle

scholarly journals Insights Into Natural Genetic Resistance to Rice Yellow Mottle Virus and Implications on Breeding for Durable Resistance

2021 ◽  
Vol 12 ◽  
Author(s):  
Patrick J. Odongo ◽  
Geoffrey Onaga ◽  
Oliver Ricardo ◽  
Keiko T. Natsuaki ◽  
Titus Alicai ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Durable Resistance ◽  
Translation Initiation Factor ◽  
Sub Saharan Africa ◽  
Initiation Factor ◽  
Mottle Virus ◽  
Rice Yellow Mottle Virus ◽  
Rice Varieties ◽  
Biotic Factor ◽  
Yellow Mottle

Rice is the main food crop for people in low- and lower-middle-income countries in Asia and sub-Saharan Africa (SSA). Since 1982, there has been a significant increase in the demand for rice in SSA, and its growing importance is reflected in the national strategic food security plans of several countries in the region. However, several abiotic and biotic factors undermine efforts to meet this demand. Rice yellow mottle virus (RYMV) caused by Solemoviridae is a major biotic factor affecting rice production and continues to be an important pathogen in SSA. To date, six pathogenic strains have been reported. RYMV infects rice plants through wounds and rice feeding vectors. Once inside the plant cells, viral genome-linked protein is required to bind to the rice translation initiation factor [eIF(iso)4G1] for a compatible interaction. The development of resistant cultivars that can interrupt this interaction is the most effective method to manage this disease. Three resistance genes are recognized to limit RYMV virulence in rice, some of which have nonsynonymous single mutations or short deletions in the core domain of eIF(iso)4G1 that impair viral host interaction. However, deployment of these resistance genes using conventional methods has proved slow and tedious. Molecular approaches are expected to be an alternative to facilitate gene introgression and/or pyramiding and rapid deployment of these resistance genes into elite cultivars. In this review, we summarize the knowledge on molecular genetics of RYMV-rice interaction, with emphasis on host plant resistance. In addition, we provide strategies for sustainable utilization of the novel resistant sources. This knowledge is expected to guide breeding programs in the development and deployment of RYMV resistant rice varieties.

scholarly journals First Report of Rice yellow mottle virus in Rice in The Gambia

Plant Disease ◽  
2008 ◽  
Vol 92 (2) ◽  
pp. 316-316 ◽  
Author(s):  
Y. Sere ◽  
F. Sorho ◽  
A. Onasanya ◽  
L. Jobe ◽  
S. Darboe ◽  
...  
Keyword(s):  
Coat Protein ◽  
Phylogenetic Reconstruction ◽  
The Gambia ◽  
Eastern Africa ◽  
Mottle Virus ◽  
Rice Yellow Mottle Virus ◽  
Leaf Extracts ◽  
Rice Varieties ◽  
Embl Accession ◽  
Yellow Mottle

Rice yellow mottle virus (RYMV) of the genus Sobemovirus is a major biotic constraint to rice (Oryza sativa) production in Africa. First reported in Kenya during 1966, RYMV was later found in most countries in Africa where rice is grown (1). In countries in westernmost Africa (The Gambia, Guinea-Bissau, Mauritania, and Senegal), plants with leaf yellowing and mottling symptoms were observed, but RYMV was never isolated. Rice is the staple food in The Gambia. In 2006, four samples were collected from local rice varieties in the Kuntaur Region in the center of The Gambia. Mechanical inoculation with leaf extracts from all samples caused typical yellow mottle symptoms on the susceptible rice varieties BG90-2, Bouaké 189, and IR64. RYMV was detected in the four samples collected by ELISA with polyclonal antisera (2). The 720-nt coat protein gene was amplified for each isolate by reverse-transcriptase-PCR with primers 5′-CAAAGATGGCCAGGAA-3′ (sense) and 5′-CTCCCCCACCCATCCCGAGAATT-3′ (antisense) (2). The RT-PCR products were directly sequenced (EMBL Accession Nos. AM765810, AM765811, AM765812, and AM765813) and then aligned using ClustalW with a pool of RYMV coat protein sequences from West African isolates (EMBL Accession Nos. AJ279905, AJ279901, AJ885137, AJ885124, and AJ279935). Phylogenetic reconstruction by maximum-likelihood with PAUP indicated that the isolates from The Gambia formed a monophyletic group with over 97% nucleotide identity and are closely related to isolates of other countries in West Africa (Burkina Faso, Côte d'Ivoire, Guinea, Mali, and Sierra-Leone) with 91 to 94% identity. Detection of RYMV in The Gambia indicates that RYMV is present in westernmost Africa, which is referred to as the ‘rice belt’ of Africa, and shows that RYMV is widely distributed from eastern Africa (Tanzania) to the western part of the continent. References: (1) N. K. Kouassi et al. Plant Dis. 89:124, 2005. (2) A. Pinel et al. Arch. Virol. 145:1621, 2000.

scholarly journals NMR chemical shift backbone assignment of the viral protein P1 encoded by the African Rice Yellow Mottle Virus

2019 ◽  
Vol 13 (2) ◽  
pp. 345-348
Author(s):  
Yinshan Yang ◽  
Vianney Poignavent ◽  
François-Xavier Gillet ◽  
Florence Vignols ◽  
Hélène Déméné
Keyword(s):  
Chemical Shift ◽  
Viral Protein ◽  
Backbone Assignment ◽  
Mottle Virus ◽  
Rice Yellow Mottle Virus ◽  
Nmr Chemical Shift ◽  
African Rice ◽  
Yellow Mottle

scholarly journals The rice yellow mottle virus P1 protein exhibits dual functions to suppress and activate gene silencing

2010 ◽  
Vol 61 (3) ◽  
pp. 371-382 ◽  
Author(s):  
Séverine Lacombe ◽  
Martine Bangratz ◽  
Florence Vignols ◽  
Christophe Brugidou
Keyword(s):  
Gene Silencing ◽  
Mottle Virus ◽  
Rice Yellow Mottle Virus ◽  
Yellow Mottle ◽  
Activate Gene ◽  
Dual Functions

Molecular epidemiology of the RNA satellite of Rice yellow mottle virus in Africa

2003 ◽  
Vol 148 (9) ◽  
pp. 1721-1733 ◽  
Author(s):  
A. Pinel ◽  
Z. Abubakar ◽  
O. Traor� ◽  
G. Konat� ◽  
D. Fargette
Keyword(s):  
Molecular Epidemiology ◽  
Mottle Virus ◽  
Rice Yellow Mottle Virus ◽  
Yellow Mottle

scholarly journals Synthesis of an infectious full-length cDNA clone of rice yellow mottle virus andmutagenesis of the coat protein

Virology ◽  
1995 ◽  
Vol 206 (1) ◽  
pp. 108-115 ◽  
Author(s):  
C. Brugidou ◽  
C. Holt ◽  
M. Ngon A Yassi ◽  
S. Zhang ◽  
R. Beachy ◽  
...  
Keyword(s):  
Coat Protein ◽  
Cdna Clone ◽  
Full Length ◽  
Mottle Virus ◽  
Rice Yellow Mottle Virus ◽  
Full Length Cdna ◽  
Yellow Mottle

scholarly journals The Genetic Basis of High Resistance to Rice Yellow Mottle Virus (RYMV) in Cultivars of Two Cultivated Rice Species

Plant Disease ◽  
1999 ◽  
Vol 83 (10) ◽  
pp. 931-935 ◽  
Author(s):  
M. N. Ndjiondjop ◽  
L. Albar ◽  
D. Fargette ◽  
C. Fauquet ◽  
A. Ghesquière
Keyword(s):  
Genetic Basis ◽  
Virus Isolate ◽  
Mottle Virus ◽  
F1 Hybrids ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rice Yellow Mottle Virus ◽  
Recessive Resistance ◽  
Cultivated Rice ◽  
Recessive Resistance Gene ◽  
Yellow Mottle

Three cultivars of Oryza sativa (IR64, Azucena, and Gigante) and four cultivars of O. glaberrima (Tog5681, Tog5673, CG14, and SG329) were evaluated for their resistance to two isolates of rice yellow mottle virus (RYMV) by enzyme-linked immunosorbent assay (ELISA) and symptomatology. Cultivars Tog5681 and Gigante were highly resistant, and no symptoms were observed when either virus isolate was inoculated at 10 or 20 days postgermination and assayed by ELISA at 7, 14, 22, 35, 50, or 64 days postinoculation. Azucena showed a partial resistance, whereas the other cultivars were susceptible. Symptom appearance was associated with increase in ELISA absorbance in the systemically infected leaves. The best discrimination among the cultivars occurred when the plants were inoculated at 10 days postgermination. Crosses were made between the highly resistant (Gigante and Tog5681) and the susceptible (IR64) cultivars to determine the genetic basis of resistance to RYMV. Evaluation of F1 hybrids and interspecific progenies, as well as the segregation of resistance in F2 and F3 lines of the IR64 × Gigante cross, provided results consistent with the presence of a single recessive resistance gene common to Tog5681 and Gigante.

scholarly journals Full-Length Genome Sequences of Recombinant and Nonrecombinant Sympatric Strains of Rice yellow mottle virus from Western Kenya

2018 ◽  
Vol 6 (8) ◽  
Author(s):  
Antony Kigaru Adego ◽  
Nils Poulicard ◽  
Agnès Pinel-Galzi ◽  
Benard Mukoye ◽  
Denis Fargette ◽  
...  
Keyword(s):  
Full Length ◽  
Mottle Virus ◽  
Rice Yellow Mottle Virus ◽  
Genome Sequences ◽  
Western Kenya ◽  
Yellow Mottle ◽  
Full Length Genome

ABSTRACT Five isolates of Rice yellow mottle virus from western Kenya were fully sequenced. One isolate of strain S4lv had been collected in 1966. Two isolates belonged to the emerging strain S4ug recently described in Uganda. Two isolates collected in 2012 are putative recombinants between the S4lv and S4ug strains.

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