Enzyme-Linked Immunosorbent Assay for Metal-Binding Proteins ofMytilus Edulis

2013 ◽  
pp. 283-290
Author(s):  
G. Roesijadi ◽  
J.E. Morris
Keyword(s):  
Immunosorbent Assay ◽  
Metal Binding ◽  
Binding Proteins ◽  
Enzyme Linked Immunosorbent Assay

Rabbit antibodies against the folate binding protein from cow's milk. Production, characterization and use for development of an enzyme-linked immunosorbent assay (ELISA)

1986 ◽  
Vol 6 (10) ◽  
pp. 895-900 ◽  
Author(s):  
Mimi Høier-Madsen ◽  
Steen Ingemann Hansen ◽  
Jan Holm
Keyword(s):  
Immunosorbent Assay ◽  
Binding Proteins ◽  
Binding Protein ◽  
Cross Reactivity ◽  
Cow’S Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Folate Binding Protein ◽  
Milk Whey ◽  
Cow's Milk ◽  
Folate Binding Proteins

Rabbit antibodies to purified folate binding protein from cow's milk whey were used for development of a two-site enzyme-linked immunosorbent assay (ELISA) for quantitation of bovine folate binding proteins, The folate binding proteins in human milk and serum showed no cross-reactivity. A partial saturation of purified bovine folate binder with folate gave rise to an increased antigenicity probably due to a ligand (folate)-induced exposure of antigenic sites on the protein.

Rabbit antibodies against the low molecular weight folate binding protein from human milk. Use for immunological characterization of human folate binding proteins in an enzyme-linked immunosorbent assay (ELISA)

1987 ◽  
Vol 7 (7) ◽  
pp. 553-557 ◽  
Author(s):  
Mimi Høier-Madsen ◽  
Steen Ingemann Hansen ◽  
Jan Holm
Keyword(s):  
Molecular Weight ◽  
Human Milk ◽  
Immunosorbent Assay ◽  
Binding Proteins ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Low Molecular Weight ◽  
Folate Binding Protein ◽  
Folate Binding Proteins

Antibodies to a low molecular weight folate binding protein isolated from human milk were raised in rabbits and used for development of a two-site enzyme-linked immunosorbent assay (ELISA) for immunological characterization of human folate binding proteins (FBPs). The high and low molecular weight FBPs from human milk were immunologically indistinguishable. Furthermore, the FBPs in human urine and cerebrospinal fluid showed a cross-reactivity of 70% and 30%, respectively. No cross-reactivity of the FBP from cow's milk was observed.

Immunochemical Quantification of Metallothioneins of a Marine Mollusc

1988 ◽  
Vol 45 (7) ◽  
pp. 1257-1263 ◽  
Author(s):  
G. Roesijadi ◽  
M. E. Unger ◽  
J. E. Morris
Keyword(s):  
Metal Binding ◽  
Binding Proteins ◽  
Polyclonal Antibodies ◽  
Exchange Chromatography ◽  
Enzyme Linked Immunosorbent Assay ◽  
Low Molecular Weight ◽  
Ammonium Sulfate Precipitation ◽  
Marine Mollusc ◽  
Peroxidase Conjugate

Mercury-induced, low molecular weight, metal-binding proteins were isolated from the marine mussel Mytilus edulis and used as antigen in the development of antibodies and an enzyme-linked immunosorbent assay (ELISA) for quantification of the proteins. Partial characterization of the isolated protein indicated that its properties were consistent with those of metallothionein (MT). In contrast with most MTs, this protein occurred as an apparent dimer and contained glycine at high levels. Polyclonal antibodies against this protein were produced in goats and purified to an IgG fraction by ammonium sulfate precipitation and DEAE ion-exchange chromatography. Ouchterlony analysis and ELISA showed that these antibodies were cross-reactive with two other charge variants of mercury-induced, metal-binding proteins of M. edulis, but not with rabbit MT. The ELISA was based on an indirect, competitive procedure utilizing a rabbit anti-goat IgG–horseradish peroxidase conjugate as the second antibody. Application of this assay to cytosolic extracts of mussel gills indicated 0.5 μg metal-binding proteins/g wet tissue weight in gills of control mussels and elevated levels up to 1780 μg/g following exposure to mercury, cadmium, or copper for 28 d. Zinc was not effective as an inducer of these proteins.

Plasminogen Interactions with Immobilized Fibrinogen

1989 ◽  
Vol 62 (04) ◽  
pp. 1078-1082 ◽  
Author(s):  
Burt Adelman ◽  
Patricia Ouynn
Keyword(s):  
Immunosorbent Assay ◽  
Aminocaproic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Regions ◽  
Dose Dependent Fashion ◽  
Epsilon Aminocaproic Acid ◽  
Dose Dependent

SummaryThis report describes the binding of plasminogen to fibrinogen adsorbed onto polystyrene wells. Binding was determined by enzyme linked immunosorbent assay. Both glu- and lys-plasminogen bound to immobilized fibrinogen in a dose-dependent fashion. However, more lys- than glu-plasminogen bound when equal concentrations of either were added to immobilized fibrinogen. Plasminogen binding was inhibited by epsilon aminocaproic acid indicating that binding was mediated via lysine-binding regions of plasminogen. Soluble fibrinogen added in excess of immobilized fibrinogen did not compete for plasminogen binding but fibrinogen fragments produced by plasmin digestion of fibrinogen did. Treatment of immobilized fibrinogen with thrombin caused a small but significant (p <0.01) increase in plasminogen binding. These studies demonstrate that immobilized fibrinogen binds both glu- and lys-plasminogen and that binding is mediated via lysine-binding regions. These interactions may facilitate plasminogen binding to fibrinogen adsorbed on to surfaces and to cells such as platelets which bind fibrinogen.

Alterations in Vascular Endothelial Cell-related Plasma Proteins In Thalassaemic Patients and their Correlation with Clinical Symptoms

1995 ◽  
Vol 74 (04) ◽  
pp. 1045-1049 ◽  
Author(s):  
P Butthep ◽  
A Bunyaratvej ◽  
Y Funahara ◽  
H Kitaguchi ◽  
S Fucharoen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Immunosorbent Assay ◽  
Plasma Proteins ◽  
Clinical Symptoms ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vascular Endothelial ◽  
Leg Ulcers

SummaryAn increased level of plasma thrombomodulin (TM) in α- and β- thalassaemia was demonstrated using an enzyme-linked immunosorbent assay (ELISA). Nonsplenectomized patients with β-thalassaemia/ haemoglobin E (BE) had higher levels of TM than splenectomized cases (BE-S). Patients with leg ulcers (BE-LU) were found to have the highest increase in TM level. Appearance of larger platelets in all types of thalassaemic blood was observed indicating an increase in the number of younger platelets. These data indicate that injury of vascular endothelial cells is present in thalassaemic patients.

Sign in / Sign up

Export Citation Format

Share Document