Uncertainties in Individual Estuary N-Loading Assessments

Author(s):  
D. A. Brock
Keyword(s):  
2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Volker Eras ◽  
Josefine Graffunder ◽  
Norus Ahmed ◽  
Jan C. Brune

Abstract Purpose Meniscus allograft transplantation (MAT) is a possible treatment for patients suffering with pain after meniscectomy. Here, peracetic acid (PAA) sterilised meniscus transplants were investigated on whether they would provide an adequate alternative to fresh-frozen transplants in their viscoelastic and mechanical properties. Methods In this analysis, 31 menisci donors (26 male and 5 female) were included. The average donor age was 49.87 years, ranging from 32 to 65 years. Menisci of matched pairs of knees underwent chemical sterilisation while counterparts were left fresh-frozen. Stiffness and load to failure were determined via suture retention. Further menisci were analysed while attached to the tibial bone block using a novel test device to mimic physiological load distribution. Meniscus relaxation, stiffness and failure loads were determined. Histology and biphasic properties of the menisci were examined and results were analysed using paired t-tests. Results A novel custom built test device allowed the application of physiological loads for suture retention testing and revealed no significant differences between PAA sterilised (14.85 ± 4.46 N/mm, 50.49 ± 17.01 N) and fresh-frozen (18.26 ± 4.46 N/mm, 59.49 ± 21.07 N) regarding stiffness and failure load, respectively. Furthermore, initial 200 N loading showed significantly higher strain in sterilised menisci (18.87 ± 1.56) compared to fresh frozen (13.81 ± 1.04). Load relaxation experiments demonstrated significantly lower relaxation for sterilised menisci (77.71 ± 1.62) compared to fresh-frozen (89.11 ± 1.00, p-value < 0.0001). Conclusion Peracetic acid sterilised human menisci performed equally to fresh-frozen counterparts in a suture retention test and in physiological failure testing providing an adequate alternative. However, meniscus relaxation, biphasic properties and strain were shown to be significantly different between the groups. A common problem of MAT is graft extrusion or shrinkage, therefore the parameters measured here should be considered and may influence meniscus extrusion after transplantation. Level of evidence n/a (experimental study)


2018 ◽  
Vol 15 (9) ◽  
pp. 2891-2907 ◽  
Author(s):  
Kateri R. Salk ◽  
George S. Bullerjahn ◽  
Robert Michael L. McKay ◽  
Justin D. Chaffin ◽  
Nathaniel E. Ostrom

Abstract. Recent global water quality crises point to an urgent need for greater understanding of cyanobacterial harmful algal blooms (cHABs) and their drivers. Nearshore areas of Lake Erie such as Sandusky Bay may become seasonally limited by nitrogen (N) and are characterized by distinct cHAB compositions (i.e., Planktothrix over Microcystis). This study investigated phytoplankton N uptake pathways, determined drivers of N depletion, and characterized the N budget in Sandusky Bay. Nitrate (NO3-) and ammonium (NH4+) uptake, N fixation, and N removal processes were quantified by stable isotopic approaches. Dissimilatory N reduction was a relatively modest N sink, with denitrification, anammox, and N2O production accounting for 84, 14, and 2 % of sediment N removal, respectively. Phytoplankton assimilation was the dominant N uptake mechanism, and NO3- uptake rates were higher than NH4+ uptake rates. Riverine N loading was sometimes insufficient to meet assimilatory and dissimilatory demands, but N fixation alleviated this deficit. N fixation made up 23.7–85.4 % of total phytoplankton N acquisition and indirectly supports Planktothrix blooms. However, N fixation rates were surprisingly uncorrelated with NO3- or NH4+ concentrations. Owing to temporal separation in sources and sinks of N to Lake Erie, Sandusky Bay oscillates between a conduit and a filter of downstream N loading to Lake Erie, delivering extensively recycled forms of N during periods of low export. Drowned river mouths such as Sandusky Bay are mediators of downstream N loading, but climate-change-induced increases in precipitation and N loading will likely intensify N export from these systems.


2001 ◽  
Vol 1 ◽  
pp. 35-41
Author(s):  
Chris J. Smith ◽  
Val O. Snow ◽  
Ray Leuning ◽  
David Hsu

The nitrogen (N) balance in a double-cropped, effluent spray irrigation system was examined for several years in southern Australia. The amounts of N added by irrigation, removed in the crop, and lost by ammonia (NH3) volatilisation, denitrification, and leaching were measured. Results from the project provide pig producers with the knowledge necessary to evaluate the efficiency of such systems for managing N, and enable sustainable effluent reuse practices to be developed. Oats were grown through the winter (May to November) without irrigation, and irrigated maize was grown during the summer/autumn (December to April). Approximately 18 mm of effluent was applied every 3 days. The effluent was alkaline (pH 8.3) and the average ammoniacal-N (NH4++ NH3) concentration was 430 mg N/l (range: 320 to 679 mg N/l). Mineral N in the 0- to 1.7-m layer tended to increase during the irrigation season and decrease during the winter/spring. About 2000 kg N/ha was found in the profile to a depth of 2 m in October 2000. N removed in the aboveground biomass (oats + maize) was 590 and 570 kg N/ha/year, equivalent to ≈25% of the applied N. Average NH3volatilisation during the daytime (6:00 to 19:00) was 2.74 kg N/ha, while volatilisation at night (19:00 to 6:00) was 0.4 kg N/ha, giving a total of 3.1 kg N/ha/day. This represents ≈12% of the N loading, assuming that these rates apply throughout the season. The balance of the N accumulated in the soil profile during the irrigation season, as 15N-labelled N studies confirmed. The high recovery of the15N-labelled N, and the comparable distribution of 15N and Br in the soil profile, implied that there was little loss of N by denitrification, even though the soil was wet enough for leaching of both tracers.


2014 ◽  
Vol 69 (9) ◽  
pp. 1805-1812 ◽  
Author(s):  
Jian Zhou ◽  
Guangxu Qin ◽  
Jianbing Zhang ◽  
Yancheng Li ◽  
Qiang He ◽  
...  

The coping strategy of a CANON (completely autotrophic nitrogen removal over nitrite) reactor working at room temperature was investigated using response surface methodology. The total nitrogen (TN) removal efficiency was taken as a dependent variable. The temperature (X), dissolved oxygen (DO) concentration (Y), and influent nitrogen loading rate (Z) were taken as independent variables. Results showed that the relation of these three independent variables can be described by the TN removal efficiency expressed as −5.03 + 1.51X + 45.16Y + 30.13Z + 0.26XY + 1.84XZ − 0.04X2 − 9.06Y2 − 99.00Z2. The analysis of variance proved that the equation is applicable. The response surface demonstrated that the temperature significantly interacts with the DO concentration and influent N loading rate. A coping strategy for the CANON reactor working at room temperature is thus proposed: altering the DO concentration and the N loading rate to counterbalance the impact of low temperature. The verification test proved the strategy is viable. The TN removal efficiency was 91.3% when the reactor was operated under a temperature of 35.0 °C, a DO of 3.0 mg/L, and a N loading rate of 0.70 kgN/(m³ d). When the temperature dropped from 35.0 to 19.2 °C, the TN removal efficiency was kept at 88.7% by regulating the influent N loading rate from 0.7 kgN/(m³ d) to 0.35 kgN/(m³ d) and the DO concentration from 3.0 to 2.6 mg/L.


1994 ◽  
Vol 51 (10) ◽  
pp. 2274-2285 ◽  
Author(s):  
Brent Wolfe ◽  
Hedy J. Kling ◽  
Gregg J. Brunskill ◽  
Paul Wilkinson

A freeze core taken from Experimental Lakes Area Lake 227 in 1988 contained 321 rhythmically paired, dark and light laminations in the upper 60.7 cm. Tape peels revealed cyclic, seasonal abundance peaks in organic and inorganic remains, which suggested that the couplets are varves. However, comparison between varve chronology and 22 yr of experimental changes in phosphorus (P) and nitrogen (N) loading and their influence on the planktonic community confirmed that the most recent varve-year estimates were 5 or 6 yr too old; this was caused by irregular sedimentation and multiple algal blooms resulting from experimental fertilization since 1969, and indistinct laminations that hampered precise couplet identification and separation. Dated horizons determined from biostratigraphic markers were used to generate compatible profiles between 1-cm slices of Lake 227 137Cs flux and reference fallout records. Nutrient concentration profiles were less helpful, as increases in carbon, N, and, P were gradual and no distinct horizon was identified as a clear marker of eutrophication. Long-term assessment of the varve chronology using 210Pb was hindered by experimental additions of 226Ra to the lake in 1970, although similar sedimentation rates from varve years 1860–1934 suggested that the varve and the deep part of the 210Pb chronologies were comparable.


SIMULATION ◽  
2020 ◽  
pp. 003754972097278
Author(s):  
Tigran A Muradyan ◽  
Nshan A Muradyan ◽  
Sergey V Verlinski ◽  
Anna Yu Poghosyan

Connecting implants with teeth is sometimes considered for the support of prostheses in partial edentulism, especially in periodontally compromised and surgical treated patients. The aim of this study is the presentation of a model of tooth–implant nonrigid fixation in comparison with implant–implant and implant–tooth rigid fixation by three-dimensional (3D) finite element analysis. As a model, a situation with a mandibular second premolar and two molars edentulism was selected. Two implantation options with three prosthetics designs were considered. The comparative analysis of stress and strain distribution values under vertical 100 and 200 N loading was performed. The highest peri-implant crestal bone stress distribution was observed in the model with the implant–tooth rigid fixation with 200 N vertical loading with results of 136.56 MPa. In the model with implant–tooth nonrigid fixation, the maximum strain value was observed in the tooth–connector zone and the stress distribution was higher in the connectors and the prosthesis pontic zone, with a maximal value of 27.77 MPa. The design of a tooth–implant fixed denture could be suggested as a method of choice for rehabilitation of the posterior edentulous segment in cases when only one distal implant could be installed. Further clinical research is required to obtain reliable validation data for the proposed method.


1992 ◽  
Vol 26 (3-4) ◽  
pp. 923-931 ◽  
Author(s):  
S. Nitisoravut ◽  
P. Y. Yang

A denitrification process was examined by using Entrapped-Mixed-Microbial Cells Immobilization (EMMCI) process. The mixed microbes were entrapped into a polymeric cellulose triacetate. The reactor was operated over one year with feed concentration ranging from 50 to 850 mg NO3-N/l. The hydraulic retention times (HRT) were also varied from 1.8 to 11.3 hours. Complete denitrification was obtained at an HRT of 2.6 to 11.3 hours. At an HRT of 1.8 hours, the maximum nitrate-N loading rate of 591.6 g/m3 ·h (based on carriers volume) was achieved with an 88.3% denitrification efficiency. Under high range of NaHCO3 concentrations from 10 to 20 g/l, the denitrification efficiency above 96% could be obtained. This EMMCI process has showed very promising results in respect to nitrate removal. It can be considered as an alternative approach tor direct treatment of nitrate-rich water or in a combination with an ion exchange process with an intent to eliminate the high nitrate concentration from the spent regenerant.


2011 ◽  
Vol 30 (2) ◽  
pp. 559-572 ◽  
Author(s):  
Rebecca A. Martin ◽  
Tamara K. Harms ◽  
Nancy B. Grimm
Keyword(s):  

2019 ◽  
Vol 99 (1) ◽  
pp. 70-79
Author(s):  
Erin L. Smith ◽  
Amanda Vosman ◽  
Lisa Kellman ◽  
Vernon Rodd

Nitrogen (N) losses from agricultural tile drainage systems are environmental and economic losses for producers. This field study quantified N losses from three reps of shallow (SD), deep/conventional (DD), and controlled drainage (CD) on farmland in Nova Scotia. Drainage systems were under corn and alfalfa–oats–clover production. Outflow water and gas samples were obtained and analyzed for nitrate and nitrous oxide. Nitrate-N loads were 5.0, 11.1, and 6.4 kg ha−1 in 2015; 1.8, 6.7, and 2.8 kg ha−1 in 2016; and 0.74, 1.8, and 1.6 kg ha−1 in 2017 for SD, DD, and CD, respectively. Controlled drainage reduced NO3−-N loading by 42.3%–58.2% when compared with the conventional/DD in 2 of 3 yr of study, whereas SD was found to reduce NO3−-N loading by 54.9%–73.1% compared with DD in all years studied. Total NO3−-N losses in this study were measured during the growing season (1 Apr. to 31 Oct.); the magnitude of NO3−-N losses and treatment effects may vary if studied year-round. Nitrous oxide fluxes were variable and low in magnitude throughout the study. Cumulated N2O losses were <1% of the applied N for all drainage types. Controlled drainage increased yields compared with SD and DD. The use of CD in the region could aid in reducing climate stresses, as well as overall NO3−-N loads exiting drainage systems and may enhance crop yields compared with conventional systems. Future studies on dissolved N2O losses from drainage water may provide important insight into whether dissolved N2O losses exceed surface emissions.


Forests ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 947
Author(s):  
Tetsuto Sugai ◽  
Satoko Yokoyama ◽  
Yutaka Tamai ◽  
Hirotaka Mori ◽  
Enrico Marchi ◽  
...  

Although compacted soil can be recovered through root development of planted seedlings, the relationship between root morphologies and soil physical properties remain unclear. We investigated the impacts of soil compaction on planted hybrid larch F1 (Larix gmelinii var. japonica×L. kaempferi, hereafter F1) seedlings with/without N loading. We assumed that N loading might increase the fine root proportion of F1 seedlings under soil compaction, resulting in less effects of root development on soil recovery. We established experimental site with different levels of soil compaction and N loading, where two-year-old F1 seedlings were planted. We used a hardness change index (HCI) to quantify a degree of soil hardness change at each depth. We evaluated root morphological responses to soil compaction and N loading, focusing on ectomycorrhizal symbiosis. High soil hardness reduced the total dry mass of F1 seedlings by more than 30%. Significant positive correlations were found between HCI and root proportion, which indicated that F1 seedling could enhance soil recovery via root development. The reduction of fine root density and its proportion due to soil compaction was observed, while these responses were contrasting under N loading. Nevertheless, the relationships between HCI and root proportion were not changed by N loading. The relative abundance of the larch-specific ectomycorrhizal fungi under soil compaction was increased by N loading. We concluded that the root development of F1 seedling accelerates soil recovery, where N loading could induce root morphological changes under soil compaction, resulting in the persistent relationship between root development and soil recovery.


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