In vitro Micropropagation of a Medicinal Plant Species Sophora flavescens

2003 ◽  
Vol 46 (1) ◽  
pp. 117-120 ◽  
Author(s):  
D.L. Zhao ◽  
G.Q. Guo ◽  
X.Y. Wang ◽  
G.C. Zheng
Keyword(s):  
Medicinal Plant ◽  
Plant Species ◽  
Medicinal Plant Species ◽  
Sophora Flavescens ◽  
In Vitro Micropropagation

scholarly journals Synthetic Seed Production as a Tool for the Conservation and Domestication of Celastrus paniculatus: A Rare Medicinal Plant

2019 ◽  
pp. 1-8
Author(s):  
D. L. C. K. Fonseka ◽  
W. W. U. I. Wickramaarachchi ◽  
R. P. S. Madushani
Keyword(s):  
Sri Lanka ◽  
Medicinal Plant ◽  
Plant Species ◽  
Synthetic Seeds ◽  
Shoot Tips ◽  
Medicinal Plant Species ◽  
Synthetic Seed ◽  
Black Oil ◽  
Celastrus Paniculatus

The black-oil tree (Celastrus paniculatus Willd) is a highly valued medicinal plant species belong to the Celastraceae family, known as Jyothishmathi in Ayurveda and Duhundu in Sri Lanka and grows as a perennial vine. It is an endangered medicinal plant species recorded in the red list of endangered fauna and flora of Sri Lanka in 1999. The seed oil of Celastrus paniculatus contains sesquiterpene alkaloids namely; celapagine, celapanigine, celapanine and celastrol, used in traditional system of medicine for various disorders and because of its high pharmaceutical value, plants are over exploited in natural habitats. Owing to poor seed germination and lack of successful vegetative propagation methods, domestication and commercial planting of this important medicinal plant species to meet the demand seems impossible. Therefore, it is of high importance to develop a reliable and efficient in vitro propagation to produce black oil plants for commercial use. In this study, it was attempted to produce synthetic seeds of Celestrus paniculatus via in vitro multiple shoot proliferation. Nodal segment explants were collected from freshly emerged age of sprouts, surface sterilized and cultured in Murashige and Skoog medium supplemented with different 6-benzylaminopurine (BAP) and Thidiazuron (TDZ) concentrations for shoot induction. The highest soot proliferation rate; 25 shoot tips/explant were observed with 0.1 mg/L TDZ. Induced shoot tips were used for synthetic seed production after encapsulating with BAP and a-naphthalene acetic (NAA) enriched sodium alginate. Shoot tip encapsulated beads produced with 4% sodium alginate were firm, clear, round and uniform in size and easy to handle. The influence of growth regulators (BAP and NAA) and storage period on the germination of encapsulated shoot tips was studied to evaluate the success of encapsulated shoot tips as a propagule. The beads germinated with 2 mg/L BAP and 0.2 mg/L NAA provided 80% in vitro germination percentage. Shoot tips of synthetic seeds remained green and healthy after storage at 5°C for a period of 8 weeks. Current findings suggest that encapsulated micro shoots (synthetic seeds) could be produced successfully, as the first step in domestication and conservation of Celastrus paniculatus. Further studies required on rooting of micro shoots, acclimatization and transferring of plantlets produced from synthetic seeds to in vivo conditions for domestication and conservation purposes.

scholarly journals Micropropagation, seed propagation and germplasm bank of Mandevilla velutina (Mart.) Woodson

2007 ◽  
Vol 64 (3) ◽  
pp. 263-268 ◽  
Author(s):  
Ronaldo Biondo ◽  
Ana Valéria Souza ◽  
Bianca Waléria Bertoni ◽  
Andreimar Martins Soares ◽  
Suzelei Castro França ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Plant Species ◽  
In Vitro Propagation ◽  
Nodal Segments ◽  
Medicinal Plant Species ◽  
Soil Conditions ◽  
Ms Medium ◽  
Germplasm Bank ◽  
Seed Propagation

Mandevilla velutina (Mart.) Woodson (Apocynaceae) is a medicinal plant species with antivenom properties, native from Brazilian Savanna regions (Cerrado), which due to overexploitation and habitat deforestation is in danger of extinction. As an initiative for conserving this endangered but economically important plant species, a micropropagation protocol was developed and genotypes were stored in the Germplasm Bank "Cerrado In vitro". For the in vitro propagation of M. velutina, nodal segments were inoculated on Murashige and Skoog (MS) medium supplemented with different concentrations of BA, Zeatin, 2ip, DTT and TDZ. Best multiplication ratio was achieved when to the medium 0.44 µM BA, ranging 1: 6.7, were added. Plantlets cultured on MS/2 medium supplemented with 26.85 µM NAA rooted successfully (50.5%). Although rooted and un-rooted plantlets acclimatized to soil conditions, great losses were observed within un-rooted plantlets, while the rooted presented 100 % survival. It was possible to maintain 43% of the M. velutina germplasm under healthy conditions for six months, with no subcultures, using the MS medium supplemented with 2% sucrose, 13.8 mM spermidine, 2% sorbitol and 2% dextrose.

scholarly journals Anticholinesterase Activity of Eight Medicinal Plant Species: In Vitro and In Silico Studies in the Search for Therapeutic Agents against Alzheimer’s Disease

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Md Josim Uddin ◽  
Daniela Russo ◽  
Md Mahbubur Rahman ◽  
Shaikh Bokhtear Uddin ◽  
Mohammad A. Halim ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Medicinal Plant ◽  
Plant Species ◽  
Inhibitory Effect ◽  
Medicinal Plant Species ◽  
Inhibitory Effects ◽  
In Silico Studies ◽  
Blumea Lacera

Many Bangladeshi medicinal plants have been used to treat Alzheimer’s disease and other neurodegenerative diseases. In the present study, the anticholinesterase effects of eight selected Bangladeshi medicinal plant species were investigated. Species were selected based on the traditional uses against CNS-related diseases. Extracts were prepared using a gentle cold extraction method. In vitro cholinesterase inhibitory effects were measured by Ellman’s method in 96-well microplates. Blumea lacera (Compositae) and Cyclea barbata (Menispermaceae) were found to have the highest acetylcholinesterase inhibitory (IC50, 150 ± 11 and 176 ± 14 µg/mL, respectively) and butyrylcholinesterase inhibitory effect (IC50, 297 ± 13 and 124 ± 2 µg/mL, respectively). Cyclea barbata demonstrated competitive inhibition, where Blumea lacera showed an uncompetitive inhibition mode for acetylcholinesterase. Smilax guianensis (Smilacaceae) and Byttneria pilosa (Malvaceae) were also found to show moderate AChE inhibition (IC50, 205 ± 31 and 221 ± 2 µg/mL, respectively), although no significant BChE inhibitory effect was observed for extracts from these plant species. Among others, Thunbergia Grandiflora (Acanthaceae) and Mikania micrantha (Compositae) were found to display noticeable AChE (IC50, 252 ± 22 µg/mL) and BChE (IC50, 314 ± 15 µg/mL) inhibitory effects, respectively. Molecular docking experiment suggested that compounds 5-hydroxy-3,6,7,3′,4′-pentamethoxyflavone (BL4) and kaempferol-3-O-α-L-rhamnopyranosyl-(1⟶6)-β-D-glucopyranoside (BL5) from Blumea lacera bound stably to the binding groove of the AChE and BChE by hydrogen-bond interactions, respectively. Therefore, these compounds could be candidates for cholinesterase inhibitors. The present findings demonstrated that Blumea lacera and Cyclea barbata are interesting objects for further studies aiming at future therapeutics for Alzheimer’s disease.

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