In vitro real-time characterization of cell attachment and spreading

1998 ◽  
Vol 9 (12) ◽  
pp. 785-788 ◽  
Author(s):  
C. FREDRIKSSON ◽  
S. KHILMAN ◽  
B. KASEMO ◽  
D. M. STEEL
Keyword(s):  
Real Time ◽  
Cell Attachment

Fabrication and Characterization of Mechanically Reinforced Collagen Sponge

2005 ◽  
Vol 288-289 ◽  
pp. 385-388
Author(s):  
Yosuke Hiraoka ◽  
Ueda Hiroki ◽  
Yu Kimura ◽  
Yasuhiko Tabata
Keyword(s):  
Cell Attachment ◽  
Average Pore Size ◽  
Collagen Sponge ◽  
Average Pore ◽  
Culture Studies ◽  
Collagen Solution ◽  
Freeze Dried ◽  
Interconnected Pore

This study describes an investigation of collagen sponge mechanically reinforced through the incorporation of poly(glycolic acid)(PGA) fiber. A collagen solution with PGA fiber homogeneously dispersed was freeze-dried, followed by dehydrothermal cross-linking to obtain collagen sponges incorporating PGA fiber. A collagen sponge without PGA fiber was prepared similarly by using the collagen solution. By scanning electron observation, the collagen sponges exhibited isotropic and interconnected pore structures with an average pore size of 180 µm, irrespective of PGA fiber incorporation. As expected, PGA fiber incorporation enabled the collagen sponge to significantly enhance the compression strength. In vitro cell culture studies revealed that the number of L929 fibroblasts initially attached was significantly greater for the collagen sponge incorporating PGA fiber than for the collagen sponge. In vitro cell proliferation studies revealed that the proliferation of cell was higher for the collagen sponge incorporating PGA fiber, by day 21, than the collagen sponge without PGA fiber. It is possible that shrinkage suppression results in the superior cell attachment and proliferation of sponge incorporating PGA fiber. After subcutaneous implantation into the backs of mice, the residual volume of collagen sponge incorporating PGA fiber was significantly large compared with that of collagen sponge. We concluded that the incorporation of PGA fiber is a simple way to reinforce collagen sponge without impairing the biocompatibility.

In Vitro Characterization of Thermal Behaviour and Bone Marrow Stromal Cell Attachment on Chitosan/Polycaprolactone (CS/PCL) Nanofibrous Scaffolds

2015 ◽  
Vol 7 (11) ◽  
pp. 2427-2435 ◽  
Author(s):  
Mohammed Fayez Al Rez ◽  
H. Fouad ◽  
Ezzedine Laourine ◽  
Martin Hild ◽  
Dilbar Aibibu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Thermal Behaviour ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Cell Attachment ◽  
Marrow Stromal Cell ◽  
Nanofibrous Scaffolds

scholarly journals Aloe Vera/Collagen Mixture Induces Integrin α1β1 and PECAM-1Genes Expression in Human Adipose-Derived Stem Cells

2019 ◽  
Vol 9 (4) ◽  
pp. 662-667 ◽  
Author(s):  
Faraz Sigaroodi ◽  
Hajar Shafaei ◽  
Mohammad Karimipour ◽  
Mohammad Amin Dolatkhah ◽  
Abbas Delazar
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Viability ◽  
Real Time ◽  
Cell Attachment ◽  
Aloe Vera ◽  
Therapeutic Effects ◽  
Adipose Derived Stem Cells ◽  
Genes Expression

Purpose: Natural biomaterials are a key base in tissue engineering, and collagen, as the maincontent of the extracellular matrix (ECM), is frequently used in tissue engineering. Aloe verahas some therapeutic effects on ulcers, therefore, the use of this natural resource has alwaysbeen considered for improving collagen function. We aimed to evaluate the effect of Aloe vera/Collagen blended on cell viability, cell attachment, and angiogenic potential by determining ofintegrin α1β1 and platelet endothelial cell adhesion molecule (PECAM-1) genes expression inhuman adipose-derived stem cells (hASCs).Methods: In this study, hASCs after harvesting of adipose tissues from abdominal subcutaneousadipose tissue and isolation, were cultured in four groups of control, collagen gel, Aloe veragel, and Aloe vera/collagen blended in vitro environment at 24h and then cell viability wasassessed by MTT (3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium) assay. Integrin α1β1 andPECAM-1 genes expression were evaluated by real-time RT-PCR.Results: The results of MTT showed that the combination of Aloe vera/collagen was retained thecell viability at the normal range and improved it. In real-time RT-PCR results, integrin α1β1 andPECAM-1 gene expression were increased in the Aloe vera/collagen blended group comparedto the control group.Conclusion: For tissue engineering purposes, Aloe vera improves collagen properties in theculture of hASCs by increasing the expression of the integrin α1β1 and PECAM-1 genes.<br />

In vitro real-time aging and characterization of poly (L/D-lactic acid)

2002 ◽  
Author(s):  
A.S. Koelling ◽  
N.J. Ballintyn ◽  
A. Salehi ◽  
D.J. Darden ◽  
M.E. Taylor ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Real Time

scholarly journals Characterization of Spontaneous and TGF-β-Induced Cell Motility of Primary Human Normal and Neoplastic Mammary Cells In Vitro Using Novel Real-Time Technology

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56591 ◽  
Author(s):  
Katharina Mandel ◽  
Daniel Seidl ◽  
Dirk Rades ◽  
Hendrik Lehnert ◽  
Frank Gieseler ◽  
...  
Keyword(s):  
Cell Motility ◽  
Real Time ◽  
Mammary Cells

Engineered Microtissues for Real-Time Characterization of Cardiomyocyte Function

2013 ◽  
Author(s):  
Ariane C. C. van Spreeuwel ◽  
Noortje A. M. Bax ◽  
Jasper Foolen ◽  
M. A. Borochin ◽  
Daisy W. J. van der Schaft ◽  
...  
Keyword(s):  
Real Time ◽  
Cardiac Tissue ◽  
Mechanical Performance ◽  
Diagnostic Tools ◽  
Cardiac Physiology ◽  
Surrounding Matrix ◽  
Speed Up ◽  
Tissue Models

Engineered cardiac tissue models become increasingly important for understanding normal and disease cardiac physiology [1]. Where clinical diagnostic tools usually measure overall function of the heart, cardiac tissue models make it possible to focus on single CMs and their microenvironment. The use of in-vitro cardiac disease models can give more insight in the functionality changes of CMs during disease and thereby speed up the development of new therapies. Therefore, we aim to develop a model for healthy and diseased myocardium to study the effect of diseased microenvironments on the mechanical performance of CMs. The platform consists of 3D engineered microtissues with matrix, CMs and fibroblasts (FBs) on an array of polydimethylsiloxane (PDMS) microposts and allows for real-time characterization of CMs and their surrounding matrix. The design was adapted from Legant et. al. [2] and enables us to measure inhomogeneous tissue forces which may occur if not all cells contract equally. Here we focus on optimization and validation of the platform to measure contraction forces and gain insight in CM mechanical functioning.

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