Frameshift Mutagenesis Induced inEscherichia coliafterin VitroTreatment of Double-Stranded DNA with Methylene Blue plus White Light:  Evidence for the Involvement of Lesion(s) Other than 8-Oxo-7,8-dihydro-2‘-deoxyguanosine

1997 ◽  
Vol 10 (5) ◽  
pp. 568-574 ◽  
Author(s):  
Jérôme Wagner ◽  
Robert P. P. Fuchs
2017 ◽  
Vol 20 (2) ◽  
pp. 065-070
Author(s):  
Erkan Dogru ◽  
Elif Erhan ◽  
Osman Atilla Arikan

This paper focuses on the dilution agent effect onto interactions between methylene blue (MB) and DNA using carbon fiber microelectrode (CFME) based DNA biosensor. In this study, designed CFME based DNA biosensor was successfully carried out considering the effect of probe dilution agent (e.g. 3-mercaptopropionic acid) against proposed hybridization mechanism types. The voltammetric signals of MB were measured at bare CFME, single-stranded DNA (ssDNA)-modified CFME and double-stranded DNA (dsDNA)-modified CFME by means of square wave voltammetry (SWV). The electrochemical parameters for MB on binding to DNA onto single CFME in the solution and at the electrode surface were described. This study shows that probe dilution agent is a significant factor to determine the type of DNA hybridization mechanisms.


2013 ◽  
Vol 49 (3) ◽  
pp. 631-639 ◽  
Author(s):  
Yang Chunhui ◽  
Bian Guohui ◽  
Yang Hong ◽  
Xiao Xiaopu ◽  
Bai Zherong ◽  
...  

2011 ◽  
Vol 32 (11) ◽  
pp. 1130-1132 ◽  
Author(s):  
Salim Ismail ◽  
Stefano Perni ◽  
Jonathan Pratten ◽  
Ivan Parkin ◽  
Michael Wilson

Silicone polymers containing the light-activated antimicrobial agent methylene blue with or without gold nanoparticles were evaluated for their ability to reduce the microbial load on surfaces in a clinical environment. When irradiated with white light, polymers containing nanogold were more effective in this respect than those containing only methylene blue.


Insects ◽  
2018 ◽  
Vol 9 (3) ◽  
pp. 109 ◽  
Author(s):  
Alessandra Lima ◽  
Cicera Silva ◽  
Cynthia Caires ◽  
Esmael Prado ◽  
Luciana Rocha ◽  
...  

Aedes aegypti (Ae. aegypti) is a competent vector for transmitting important viral diseases such as yellow fever, dengue, chikungunya, and Zika. Several strategies have been applied to avoid Ae. aegypti proliferation by using environmental management, biological, and chemical approaches. However, the development of new methods for effective control of the insect vector population is still needed. Photodynamic control is an alternative way to control the vector population by using a physical approach based on the larval phototoxicity of a photosensitizer. In this context, the present study evaluated the use of eosin-methylene blue (EMB) as a new photosensitizer for photodynamic control of Ae. aegypti larval populations. The photodynamic assays were performed submitting Ae. aegypti third-instar larvae to different EMB concentrations (0.0, 0.5, 1.0, 5.0, 10.0, 50.0, and 100.0 µg mL−1) in combination of three different light doses (24.3, 48.6, and 97.2 J cm−2) under either white-light radiation from RGB LEDs or sunlight. The results demonstrated that EMB presented a rapid internalization into the larvae and was phototoxic. The photodynamic action induced 100% of larval mortality after about 40 min of sunlight irradiation even using low EMB concentration (0.5 µg mL−1). The findings reveal EMB as an effective photoactive compound to control larval populations of Ae. aegypti by photodynamic process induced by either sunlight or white-light from RGB LEDs.


2006 ◽  
Vol 988 ◽  
Author(s):  
Jennifer I. L. Chen ◽  
Georg von Freymann ◽  
Sung Yeun Choi ◽  
Vladimir Kitaev ◽  
Geoffrey A. Ozin

AbstractWe demonstrate monochromatic and white light optical amplification of the photo-oxidation of adsorbed methylene blue using an inverse colloidal photonic crystal fashioned from anatase nanocrystals, denoted i-nc-TiO2-o. Enhanced photo-activity that drives the oxidation of the dye is attributed to slow photons in i-nc-TiO2-o. When the slow photon wavelength is optimized with respect to the electronic excitation energy of i-nc-TiO2-o, the photo-oxidation rate of the dye is doubled compared to conventional nc-TiO2. By increasing the probability of absorbing photons in i-nc-TiO2-o relative to nc-TiO2, a larger population of electron-hole pairs is generated enabling more efficient photo-oxidation. Slow photons in photonic crystals portend a myriad of opportunities for amplified photo-processes in chemistry and biology.


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