Reactive Oxygen Species May Participate in the Mutagenicity and Mutational Spectrum of Cadmium in Chinese Hamster Ovary-K1 Cells

1996 ◽  
Vol 9 (8) ◽  
pp. 1360-1367 ◽  
Author(s):  
Jia-Ling Yang ◽  
Jui-I Chao ◽  
Jin-Guo Lin
Keyword(s):  
Reactive Oxygen Species ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Mutational Spectrum

scholarly journals 5-Hydroxytryptamine1A receptor/Giβγ stimulates mitogen-activated protein kinase via NAD(P)H oxidase and reactive oxygen species upstream of Src in Chinese hamster ovary fibroblasts

2000 ◽  
Vol 347 (1) ◽  
pp. 61-67 ◽  
Author(s):  
Yurii V. MUKHIN ◽  
Maria N. GARNOVSKAYA ◽  
Georgiann COLLINSWORTH ◽  
Jasjit S. GREWAL ◽  
DeKisha PENDERGRASS ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Reactive Oxygen ◽  
Mitogen Activated Protein Kinase ◽  
Oxygen Species ◽  
Erk Activation ◽  
Activation Pathway

The hypothesis of this work is that the ‘serotonin’ or 5-hydroxytryptamine (5-HT)1A receptor, which activates the extracellular signal-regulated kinase (ERK) through a Giβγ-mediated pathway, does so through the intermediate actions of reactive oxygen species (ROS). Five criteria were shown to support a key role for ROS in the activation of ERK by the 5-HT1A receptor. (1) Antioxidants inhibit activation of ERK by 5-HT. (2) Application of cysteine-reactive oxidant molecules activates ERK. (3) The 5-HT1A receptor alters cellular redox properties, and generates both superoxide and hydrogen peroxide. (4) A specific ROS-producing enzyme [NAD(P)H oxidase] is involved in the activation of ERK. (5) There is specificity both in the effects of various chemical oxidizers, and in the putative location of the ROS in the ERK activation pathway. We propose that NAD(P)H oxidase is located in the ERK activation pathway stimulated by the transfected 5-HT1A receptor in Chinese hamster ovary (CHO) cells downstream of Giβγ subunits and upstream of or at the level of the non-receptor tyrosine kinase, Src. Moreover, these experiments provide confirmation that the transfected human 5-HT1A receptor induces the production of ROS (superoxide and hydrogen peroxide) in CHO cells, and support the possibility that an NAD(P)H oxidase-like enzyme might be involved in the 5-HT-mediated generation of both superoxide and hydrogen peroxide.

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