scholarly journals Mapping DNA Adducts of Mitomycin C and Decarbamoyl Mitomycin C in Cell Lines Using Liquid Chromatography/Electrospray Tandem Mass Spectrometry

2008 ◽  
Vol 21 (12) ◽  
pp. 2370-2378 ◽  
Author(s):  
Manuel M. Paz ◽  
Sweta Ladwa ◽  
Elise Champeil ◽  
Yanfeng Liu ◽  
Sara Rockwell ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Mitomycin C ◽  
Cell Lines ◽  
Dna Adducts ◽  
Tandem Mass ◽  
Electrospray Tandem Mass Spectrometry

Analysis of Brassinosteroids in Soybean Seeds and Leaves by Liquid Chromatography-Tandem Mass Spectrometry

2017 ◽  
Vol 10 (1) ◽  
pp. 100-109
Author(s):  
Hongxia Li ◽  
Natasha Trzaskalski ◽  
R.J. Neil Emery
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
Soybean Seeds ◽  
Tandem Mass ◽  
Electrospray Tandem Mass Spectrometry ◽  
Aqueous Methanol ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Objective:A simple and fast high performance liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) method has been developed for the analysis of brassinosteroids (BRs) in plants without derivatization.Materials:The BRs (including castasterone, 24-epicastasterone, brassinolide and 24-epibrassinolide) have been extracted with ice cold 80% aqueous methanol solution.Method:Five different purification strategies have been tested for the purification and enrichment of BRs.Conclusion:This analytical method was sensitive, reliable, rapid and applicable to trace analysis in complex plant samples.

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