Prediction of Drug-Induced Liver Injury in HepG2 Cells Cultured with Human Liver Microsomes

2015 ◽  
Vol 28 (5) ◽  
pp. 872-885 ◽  
Author(s):  
Jong Min Choi ◽  
Soo Jin Oh ◽  
Ji-Yoon Lee ◽  
Jang Su Jeon ◽  
Chang Seon Ryu ◽  
...  
Keyword(s):  
Liver Injury ◽  
Hepg2 Cells ◽  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Drug Induced ◽  
Drug Induced Liver Injury ◽  
Cells Cultured

Metabolism of Ebracteolata Compound B Studied In Vitro with Human Liver Microsomes, HepG2 Cells, and Recombinant Human Enzymes

2010 ◽  
Vol 38 (12) ◽  
pp. 2157-2165 ◽  
Author(s):  
Xia Zhang ◽  
Yan Yao ◽  
Yan Lou ◽  
Huidi Jiang ◽  
Xiaowen Wang ◽  
...  
Keyword(s):  
Hepg2 Cells ◽  
Human Liver ◽  
Liver Microsomes ◽  
Human Liver Microsomes

scholarly journals Advances in Engineered Liver Models for Investigating Drug-Induced Liver Injury

2016 ◽  
Vol 2016 ◽  
pp. 1-20 ◽  
Author(s):  
Christine Lin ◽  
Salman R. Khetani
Keyword(s):  
Liver Injury ◽  
Human Liver ◽  
Animal Studies ◽  
Drug Exposure ◽  
Induced Pluripotent Stem Cell ◽  
Cell Types ◽  
Rapid Decline ◽  
Cellular Mechanisms ◽  
Drug Induced ◽  
Drug Induced Liver Injury

Drug-induced liver injury (DILI) is a major cause of drug attrition. Testing drugs on human liver models is essential to mitigate the risk of clinical DILI since animal studies do not always suffice due to species-specific differences in liver pathways. While primary human hepatocytes (PHHs) can be cultured on extracellular matrix proteins, a rapid decline in functions leads to low sensitivity (<50%) in DILI prediction. Semiconductor-driven engineering tools now allow precise control over the hepatocyte microenvironment to enhance and stabilize phenotypic functions. The latest platforms coculture PHHs with stromal cells to achieve hepatic stability and enable crosstalk between the various liver cell types towards capturing complex cellular mechanisms in DILI. The recent introduction of induced pluripotent stem cell-derived human hepatocyte-like cells can potentially allow a better understanding of interindividual differences in idiosyncratic DILI. Liver models are also being coupled to other tissue models via microfluidic perfusion to study the intertissue crosstalk upon drug exposure as in a live organism. Here, we review the major advances being made in the engineering of liver models and readouts as they pertain to DILI investigations. We anticipate that engineered human liver models will reduce drug attrition, animal usage, and cases of DILI in humans.

Evaluation of 3-D bioprinted human liver tissue for assessment drug-induced liver injury across a diverse set of chemical classes

2017 ◽  
Vol 280 ◽  
pp. S270
Author(s):  
Jeffrey Irelan ◽  
Candace Crogan-Grundy ◽  
Ryan Smith ◽  
Rhiannon Hardwick ◽  
Deborah Nguyen
Keyword(s):  
Liver Injury ◽  
Liver Tissue ◽  
Human Liver ◽  
Drug Induced ◽  
Human Liver Tissue ◽  
Drug Induced Liver Injury

Evaluation of the Potential for Drug-Induced Liver Injury Based on in Vitro Covalent Binding to Human Liver Proteins

2009 ◽  
Vol 37 (12) ◽  
pp. 2383-2392 ◽  
Author(s):  
Toru Usui ◽  
Masashi Mise ◽  
Takanori Hashizume ◽  
Masashi Yabuki ◽  
Setsuko Komuro
Keyword(s):  
Liver Injury ◽  
Human Liver ◽  
Covalent Binding ◽  
Drug Induced ◽  
Drug Induced Liver Injury

Can Galactose Be Converted to Glucose in HepG2 Cells? Improving thein VitroMitochondrial Toxicity Assay for the Assessment of Drug Induced Liver Injury

2019 ◽  
Vol 32 (8) ◽  
pp. 1528-1544 ◽  
Author(s):  
Qiuwei Xu ◽  
Liping Liu ◽  
Heather Vu ◽  
Matthew Kuhls ◽  
Amy G. Aslamkhan ◽  
...  
Keyword(s):  
Liver Injury ◽  
Hepg2 Cells ◽  
Toxicity Assay ◽  
Drug Induced ◽  
Drug Induced Liver Injury
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