An Approach to Investigate Intracellular Protein Network Responses

Holly N. Currie; Julie A. Vrana; Alice A. Han; Giovanni Scardoni; Nate Boggs; Jonathan W. Boyd

doi:10.1021/tx400247g

An Approach to Investigate Intracellular Protein Network Responses

Chemical Research in Toxicology ◽

10.1021/tx400247g ◽

2014 ◽

Vol 27 (1) ◽

pp. 17-26 ◽

Cited By ~ 8

Author(s):

Holly N. Currie ◽

Julie A. Vrana ◽

Alice A. Han ◽

Giovanni Scardoni ◽

Nate Boggs ◽

...

Keyword(s):

Protein Network ◽

Intracellular Protein

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Disease genes and intracellular protein networks

Physiological Genomics ◽

10.1152/physiolgenomics.00095.2003 ◽

2003 ◽

Vol 15 (3) ◽

pp. 223-227 ◽

Cited By ~ 14

Author(s):

S. Bortoluzzi ◽

C. Romualdi ◽

A. Bisognin ◽

G. A. Danieli

Keyword(s):

Protein Network ◽

Cdna Libraries ◽

Protein Networks ◽

Disease Genes ◽

Intracellular Protein ◽

Scale Free ◽

Specific Distribution ◽

Human Genes ◽

Genome Level ◽

Important Nodes

By a computational approach we reconstructed genomic transcriptional profiles of 19 different adult human tissues, based on information on activity of 27,924 genes obtained from unbiased UniGene cDNA libraries. In each considered tissue, a small number of genes resulted highly expressed or “tissue specific.” Distribution of gene expression levels in a tissue appears to follow a power law, thus suggesting a correspondence between transcriptional profile and “scale-free” topology of protein networks. The expression of 737 genes involved in Mendelian diseases was analyzed, compared with a large reference set of known human genes. Disease genes resulted significantly more expressed than expected. The possible correspondence of their products to important nodes of intracellular protein network is suggested. Auto-organization of the protein network, its stability in time in the differentiated state, and relationships with the degree of genetic variability at genome level are discussed.

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Androgen-induced plasticity at a “vocal” neuromuscular synapse

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100167895 ◽

1994 ◽

Vol 52 ◽

pp. 32-33

Author(s):

Darcy B. Kelley ◽

Martha L. Tobias ◽

Mark Ellisman

Keyword(s):

Xenopus Laevis ◽

Motor Neurons ◽

Sexual Differentiation ◽

Molecular Basis ◽

Neuromuscular Synapse ◽

Sexually Dimorphic ◽

Intracellular Protein ◽

Developmental Program ◽

Androgen Action ◽

Clawed Frog

Brain and muscle are sexually differentiated tissues in which masculinization is controlled by the secretion of androgens from the testes. Sensitivity to androgen is conferred by the expression of an intracellular protein, the androgen receptor. A central problem of sexual differentiation is thus to understand the cellular and molecular basis of androgen action. We do not understand how hormone occupancy of a receptor translates into an alteration in the developmental program of the target cell. Our studies on sexual differentiation of brain and muscle in Xenopus laevis are designed to explore the molecular basis of androgen induced sexual differentiation by examining how this hormone controls the masculinization of brain and muscle targets.Our approach to this problem has focused on a highly androgen sensitive, sexually dimorphic neuromuscular system: laryngeal muscles and motor neurons of the clawed frog, Xenopus laevis. We have been studying sex differences at a synapse, the laryngeal neuromuscular junction, which mediates sexually dimorphic vocal behavior in Xenopus laevis frogs.

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