scholarly journals Quantification of Acylfulvene– and Illudin S–DNA Adducts in Cells with Variable Bioactivation Capacities

2012 ◽  
Vol 26 (1) ◽  
pp. 146-155 ◽  
Author(s):  
Kathryn E. Pietsch ◽  
Paul M. van Midwoud ◽  
Peter W. Villalta ◽  
Shana J. Sturla
Keyword(s):  
Dna Adducts ◽  
Illudin S ◽  
In Cells

Formation of DNA Adducts by the Anticancer Drug Carboplatin: Different Nucleotide Sequence Preferences in Vitro and in Cells

Biochemistry ◽  
1995 ◽  
Vol 34 (26) ◽  
pp. 8474-8480 ◽  
Author(s):  
Frank A. Blommaert ◽  
Helma C. M. van Dijk-Knijnenburg ◽  
Fransje J. Dijt ◽  
Leo den Engelse ◽  
Robert A. Baan ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Anticancer Drug ◽  
Dna Adducts ◽  
In Cells

scholarly journals N-acetylcysteine blocks formation of cancer-initiating estrogen–DNA adducts in cells

2010 ◽  
Vol 49 (3) ◽  
pp. 392-400 ◽  
Author(s):  
Muhammad Zahid ◽  
Muhammad Saeed ◽  
Mohammed F. Ali ◽  
Eleanor G. Rogan ◽  
Ercole L. Cavalieri
Keyword(s):  
Dna Adducts ◽  
In Cells

Measurement of DNA adducts in cells exposed to cisplatin

2004 ◽  
Vol 333 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Herbert Iijima ◽  
Helen B. Patrzyc ◽  
Jean B. Dawidzik ◽  
Edwin E. Budzinski ◽  
Han-Chun Cheng ◽  
...  
Keyword(s):  
Dna Adducts ◽  
In Cells

Formation of Transient Covalent Protein and DNA Adducts by Quercetin in Cells with and without Oxidative Enzyme Activity

2005 ◽  
Vol 18 (12) ◽  
pp. 1907-1916 ◽  
Author(s):  
Hester van der Woude ◽  
Gerrit M. Alink ◽  
Bart E. J. van Rossum ◽  
Kristina Walle ◽  
Harry van Steeg ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Dna Adducts ◽  
Oxidative Enzyme ◽  
Oxidative Enzyme Activity ◽  
In Cells

scholarly journals Formation of stable adducts and absence of depurinating DNA adducts in cells and DNA treated with the potent carcinogen dibenzo[a,l]pyrene or its diol epoxides

1997 ◽  
Vol 94 (25) ◽  
pp. 13542-13547 ◽  
Author(s):  
V. J. Melendez-Colon ◽  
C. A. Smith ◽  
A. Seidel ◽  
A. Luch ◽  
K. L. Platt ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Diol Epoxides ◽  
Potent Carcinogen ◽  
In Cells

Identification of Dibenzo[a,l]pyrene-DNA Adducts Formed in Cells in Culture and in Mouse Skin

1994 ◽  
Vol 6 (1-4) ◽  
pp. 199-206 ◽  
Author(s):  
Sherry L. Ralston ◽  
Hudson H. S. Lau ◽  
Albrecht Seidel ◽  
Andreas Luch ◽  
Karl L. Platt ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Mouse Skin ◽  
Cells In Culture ◽  
In Cells

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

Effect of Some Metabolic Inhibitors on Vinblastine-Induced Ribosomal-Granular Material Complexes

1971 ◽  
Vol 29 ◽  
pp. 548-549
Author(s):  
Awtar Krishan ◽  
Dora Hsu
Keyword(s):  
Granular Material ◽  
Rna Synthesis ◽  
Culture Medium ◽  
Actinomycin D ◽  
Metabolic Inhibitors ◽  
Protein And Rna Synthesis ◽  
Apparent Reduction ◽  
Plant Alkaloids ◽  
In Cells

Cells exposed to antitumor plant alkaloids, vinblastine and vincristine sulfate have large proteinacious crystals and complexes of ribosomes, helical polyribosomes and electron-dense granular material (ribosomal complexes) in their cytoplasm, Binding of H3-colchicine by the in vivo crystals shows that they contain microtubular proteins. Association of ribosomal complexes with the crystals suggests that these structures may be interrelated.In the present study cultured human leukemic lymphoblasts (CCRF-CEM), were incubated with protein and RNA-synthesis inhibitors, p. fluorophenylalanine, puromycin, cycloheximide or actinomycin-D before the addition of crystal-inducing doses of vinblastine to the culture medium. None of these compounds could completely prevent the formation of the ribosomal complexes or the crystals. However, in cells pre-incubated with puromycin, cycloheximide, or actinomycin-D, a reduction in the number and size of the ribosomal complexes was seen. Large helical polyribosomes were absent in the ribosomal complexes of cells treated with puromycin, while in cells exposed to cycloheximide, there was an apparent reduction in the number of ribosomes associated with the ribosomal complexes (Fig. 2).

Detection of Nucleic Acids in Cells or Tissue Sections Using In situ Polymerase Chain Reaction (PCR)

1996 ◽  
Vol 54 ◽  
pp. 16-17
Author(s):  
J. R. Hully ◽  
K. R. Luehrsen ◽  
K. Aoyagi ◽  
C. Shoemaker ◽  
R. Abramson
Keyword(s):  
Nucleic Acids ◽  
Viral Infections ◽  
Anatomical Location ◽  
Rt Pcr ◽  
Reverse Transcription Pcr ◽  
Cellular Source ◽  
Gene Transcripts ◽  
Initial Description ◽  
In Cells

The development of PCR technology has greatly accelerated medical research at the genetic and molecular levels. Until recently, the inherent sensitivity of this technique has been limited to isolated preparations of nucleic acids which lack or at best have limited morphological information. With the obvious exception of cell lines, traditional PCR or reverse transcription-PCR (RT-PCR) cannot identify the cellular source of the amplified product. In contrast, in situ hybridization (ISH) by definition, defines the anatomical location of a gene and/or it’s product. However, this technique lacks the sensitivity of PCR and cannot routinely detect less than 10 to 20 copies per cell. Consequently, the localization of rare transcripts, latent viral infections, foreign or altered genes cannot be identified by this technique. In situ PCR or in situ RT-PCR is a combination of the two techniques, exploiting the sensitivity of PCR and the anatomical definition provided by ISH. Since it’s initial description considerable advances have been made in the application of in situ PCR, improvements in protocols, and the development of hardware dedicated to in situ PCR using conventional microscope slides. Our understanding of the importance of viral latency or viral burden in regards to HIV, HPV, and KSHV infections has benefited from this technique, enabling detection of single viral copies in cells or tissue otherwise thought to be normal. Clearly, this technique will be useful tool in pathobiology especially carcinogenesis, gene therapy and manipulations, the study of rare gene transcripts, and forensics.

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