scholarly journals Effect of Clozapine on Neutrophil Kinetics in Rabbits

2010 ◽  
Vol 23 (7) ◽  
pp. 1184-1191 ◽  
Author(s):  
Suzanne Iverson ◽  
Antti Kautiainen ◽  
Julia Ip ◽  
Jack P. Uetrecht
Keyword(s):  
Neutrophil Kinetics

Neutrophil kinetics in rabbits during infusion of zymosan-activated plasma

1989 ◽  
Vol 67 (1) ◽  
pp. 88-95 ◽  
Author(s):  
C. M. Doerschuk ◽  
M. F. Allard ◽  
J. C. Hogg
Keyword(s):  
Relative Size ◽  
Pulmonary Sequestration ◽  
Blood Velocity ◽  
Pulmonary Capillaries ◽  
In Vitro Activation ◽  
The Complement System ◽  
Kinetics In Vivo ◽  
Neutrophil Kinetics

Complement activation in vivo produces neutropenia and pulmonary sequestration of neutrophils (PMNs) whereas in vitro activation increases PMN adherence and decreases PMN deformability. The present study examined PMN kinetics in vivo to determine if this sequestration was specific to the lung. Venous or arterial injections of radiolabeled PMNs were given to animals receiving infusions of zymosan-activated plasma (ZAP) or saline, and the PMN distribution was evaluated 10 min later. In control animals, the relative size of the marginated and circulating PMN pools was similar after venous or arterial injection and regional PMN retention increased as blood velocity slowed. ZAP infusion produced threefold increases in PMNs within pulmonary capillaries after venous injection and PMN retention was independent of blood velocity. After arterial injection, ZAP infusion produced PMN sequestration in all organs. Rigid (glutaraldehyde-fixed) PMNs injected into control rabbits showed increased lung recoveries similar to those of fresh PMNs injected into ZAP-treated rabbits. We conclude that activation of the complement system causes PMN sequestration in both the pulmonary and the systemic microvasculature and that the decrease in PMN deformability that occurs with activation of the PMN may be important in the genesis of PMN sequestration.

Plasma Myeloperoxidase and Lactoferrin Measured by Radioimmunoassay: Relations to Neutrophil Kinetics

2009 ◽  
Vol 198 (1-6) ◽  
pp. 437-443 ◽  
Author(s):  
Niels Ebbe Hansen ◽  
Jörgen Malmquist ◽  
Jan Thorell
Keyword(s):  
Neutrophil Kinetics

scholarly journals A Method for Leukokinetic Study in the Nonsteady State

Blood ◽  
1971 ◽  
Vol 38 (3) ◽  
pp. 302-311 ◽  
Author(s):  
G. ROTHSTEIN ◽  
C. R. BISHOP ◽  
J. W. ATHENS ◽  
H. E. ASHENBRUCKER
Keyword(s):  
Flow Rate ◽  
Early Phase ◽  
Specific Activity ◽  
Citrate Solution ◽  
Cell Agglutination ◽  
Nonsteady State ◽  
Radioactive Assay ◽  
Neutrophil Kinetics

Abstract In these studies, the DF32P leukokinetic technique has been modified so that neutrophil kinetics may be studied in the nonsteady state. In order to achieve this modification, an acid citrate solution was used in preparing leukocytes for radioactive assay. This results in a suspension free of cell agglutination so that a specific activity related only to neutrophils is obtained. This technique has been successfully employed in the study of the early phase of cortisol-induced granulocytosis, allowing measurement of cell flow rate in and out of the circulating granulocyte pool. This method is also useful in the study of subjects with pronounced neutropenia.

Neutrophil kinetics during active cigarette smoking in rabbits

1991 ◽  
Vol 71 (2) ◽  
pp. 630-637 ◽  
Author(s):  
C. H. Bosken ◽  
C. M. Doerschuk ◽  
D. English ◽  
J. C. Hogg
Keyword(s):  
Cardiac Output ◽  
New Zealand ◽  
Cigarette Smoke ◽  
Smoke Exposure ◽  
Pulmonary Vasculature ◽  
Pulmonary Blood Volume ◽  
Pulmonary Capillary ◽  
New Zealand White Rabbits ◽  
Capillary Bed ◽  
Neutrophil Kinetics

Polymorphonuclear leukocyte (PMN) transit through the pulmonary vasculature is slowed during inhalation of cigarette smoke in humans. This study was undertaken to determine the localization of the delayed PMN and whether they release granule-bound enzymes during smoke exposure. Anesthetized New Zealand White rabbits were exposed to cigarette smoke (n = 5) or sham (n = 5) for 10 min while they breathed spontaneously. The cardiac output, pulmonary blood volume and flow, and PMN retention were measured in each of five gravity-defined slices of lung. In three smoke-exposed and three sham animals the lungs were prepared for autoradiography, and the distribution of the radiolabeled PMN was determined. Plasma was assayed for myeloperoxidase in 10 animals. We found that smoke exposure caused increased PMN retention in the top two slices of the lungs without changing hemodynamics. The PMN were randomly distributed in the lobule, and plasma myeloperoxidase was elevated at the beginning of the exposure. We conclude that cigarette smoke may damage the lung by activating PMN in the pulmonary capillary bed.

Neutrophil Kinetics in Acute Bacterial Infection

2009 ◽  
Vol 204 (1-6) ◽  
pp. 407-412 ◽  
Author(s):  
Niels Ebbe Hansen ◽  
Hans Karle ◽  
Niels Henrik Valerius
Keyword(s):  
Bacterial Infection ◽  
Neutrophil Kinetics

Neutrophil Kinetics in the Pulmonary Microcirculation: Effects of Pressure and Flow in the Dependent Lung

1990 ◽  
Vol 141 (4_pt_1) ◽  
pp. 953-959 ◽  
Author(s):  
Dale C. Lien ◽  
G. Scott Worthen ◽  
Ronald L. Capen ◽  
Wendy L. Hanson ◽  
Lori L. Checkley ◽  
...  
Keyword(s):  
Dependent Lung ◽  
Pulmonary Microcirculation ◽  
Neutrophil Kinetics

scholarly journals The Effect of Hybridoma Antibody Administration upon Neutrophil Kinetics during Experimental Type III Group B Streptococcal Sepsis

1983 ◽  
Vol 17 (10) ◽  
pp. 795-799 ◽  
Author(s):  
Robert D Christensen ◽  
Gerald Rothstein ◽  
Harry R Hill ◽  
Seth H Pincus
Keyword(s):  
Type Iii ◽  
Experimental Type ◽  
Streptococcal Sepsis ◽  
Group B ◽  
Neutrophil Kinetics

The Effect of Cigarette Smoking on Neutrophil Kinetics in Human Lungs

1989 ◽  
Vol 321 (14) ◽  
pp. 924-928 ◽  
Author(s):  
William MacNee ◽  
Barry Wiggs ◽  
Alan S. Belzberg ◽  
James C. Hogg
Keyword(s):  
Cigarette Smoking ◽  
Human Lungs ◽  
Neutrophil Kinetics

Pulmonary neutrophil kinetics

1990 ◽  
Vol 11 (4A) ◽  
pp. 133-139 ◽  
Author(s):  
William MacNee
Keyword(s):  
Neutrophil Kinetics

Positron emission tomography with [18F]fluorodeoxyglucose to evaluate neutrophil kinetics during acute lung injury

2004 ◽  
Vol 286 (4) ◽  
pp. L834-L840 ◽  
Author(s):  
Delphine L. Chen ◽  
Daniel P. Schuster
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Pet Imaging ◽  
Fdg Pet ◽  
Pulmonary Sequestration ◽  
Fdg Uptake ◽  
Positron Emission ◽  
Activated Neutrophils ◽  
Neutrophil Kinetics

We measured neutrophil glucose uptake with positron emission tomographic imaging and [18F]fluorodeoxyglucose ([18F]FDG-PET) in anesthetized dogs after intravenous oleic acid-induced acute lung injury (ALI; OA group, n = 6) or after low-dose intravenous endotoxin (known to activate neutrophils without causing lung injury) followed by OA (Etx + OA group, n = 7). The following two other groups were studied as controls: one that received no intervention ( n = 5) and a group treated with Etx only ( n = 6). PET imaging was performed ∼1.5 h after initiating experimental interventions. The rate of [3H]deoxyglucose ([3H]DG) uptake was also measured in vitro in cells recovered from bronchoalveolar lavage (BAL) performed after PET imaging. Circulating neutrophil counts fell significantly in animals treated with Etx but not in the other two groups. The rate of [18F]FDG uptake, measured by the influx constant Ki, was significantly elevated ( P < 0.05) in both Etx-treated groups (7.9 ± 2.6 × 10-3ml blood·ml lung-1·min-1in the Etx group, 9.3 ± 4.8 × 10-3ml blood·ml lung-1·min-1in the Etx + OA group) but not in the group treated only with OA (3.4 ± 0.8 × 10-3ml blood·ml lung-1·min-1) when compared with the normal control (1.6 ± 0.4 × 10-3ml blood·ml lung-1·min-1). [3H]DG uptake was increased (73 ± 7%) in BAL neutrophils recovered from the Etx + OA group ( P < 0.05) but not in the OA group. Kiand [3H]DG uptake rates were linearly correlated ( R2= 0.65). We conclude that the rate of [18F]FDG uptake in the lungs during ALI reflects the state of neutrophil activation. [18F]FDG-PET imaging can detect pulmonary sequestration of activated neutrophils, despite the absence of alveolar neutrophilia. Thus [18F]FDG-PET imaging may be a useful tool to study neutrophil kinetics during ALI.

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