scholarly journals Translesion Synthesis Across 1,N2-Ethenoguanine by Human DNA Polymerases

2006 ◽  
Vol 19 (6) ◽  
pp. 879-886 ◽  
Author(s):  
Jeong-Yun Choi ◽  
Hong Zang ◽  
Karen C. Angel ◽  
Ivan D. Kozekov ◽  
Angela K. Goodenough ◽  
...  
Keyword(s):  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Human Dna

Translesion Synthesis past Equine Estrogen-Derived 2‘-Deoxycytidine DNA Adducts by Human DNA Polymerases η and κ†

Biochemistry ◽  
2004 ◽  
Vol 43 (35) ◽  
pp. 11312-11320 ◽  
Author(s):  
Naomi Suzuki ◽  
Manabu Yasui ◽  
Y. R. Santosh Laxmi ◽  
Haruo Ohmori ◽  
Fumio Hanaoka ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Human Dna

Translesion Synthesis Past Estrogen-Derived DNA Adducts by Human DNA Polymerases η and κ†

Biochemistry ◽  
2004 ◽  
Vol 43 (20) ◽  
pp. 6304-6311 ◽  
Author(s):  
Naomi Suzuki ◽  
Shinji Itoh ◽  
Kinning Poon ◽  
Chikahide Masutani ◽  
Fumio Hanaoka ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Human Dna

scholarly journals Translesion Synthesis Past Tamoxifen-Derived DNA Adducts by Human DNA Polymerases η and κ†

Biochemistry ◽  
2006 ◽  
Vol 45 (39) ◽  
pp. 12167-12174 ◽  
Author(s):  
Manabu Yasui ◽  
Naomi Suzuki ◽  
Y. R. Santosh Laxmi ◽  
Shinya Shibutani
Keyword(s):  
Dna Adducts ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Human Dna

scholarly journals Translesion Synthesis acrossO6-Alkylguanine DNA Adducts by Recombinant Human DNA Polymerases

2006 ◽  
Vol 281 (50) ◽  
pp. 38244-38256 ◽  
Author(s):  
Jeong-Yun Choi ◽  
Goutam Chowdhury ◽  
Hong Zang ◽  
Karen C. Angel ◽  
Choua C. Vu ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Human Dna

scholarly journals Bypass of the Major Alkylative DNA Lesion by Human DNA Polymerase η

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3928 ◽  
Author(s):  
Myong-Chul Koag ◽  
Hunmin Jung ◽  
Yi Kou ◽  
Seongmin Lee
Keyword(s):  
Dna Polymerase ◽  
Base Pair ◽  
Alkylating Agents ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Catalytic Site ◽  
Structural Basis ◽  
Dna Lesion ◽  
Human Dna ◽  
Wide Range

A wide range of endogenous and exogenous alkylating agents attack DNA to generate various alkylation adducts. N7-methyl-2-deoxyguanosine (Fm7dG) is the most abundant alkylative DNA lesion. If not repaired, Fm7dG can undergo spontaneous depurination, imidazole ring-opening, or bypass by translesion synthesis DNA polymerases. Human DNA polymerase η (polη) efficiently catalyzes across Fm7dG in vitro, but its structural basis is unknown. Herein, we report a crystal structure of polη in complex with templating Fm7dG and an incoming nonhydrolyzable dCTP analog, where a 2′-fluorine-mediated transition destabilization approach was used to prevent the spontaneous depurination of Fm7dG. The structure showed that polη readily accommodated the Fm7dG:dCTP base pair with little conformational change of protein and DNA. In the catalytic site, Fm7dG and dCTP formed three hydrogen bonds with a Watson–Crick geometry, indicating that the major keto tautomer of Fm7dG is involved in base pairing. The polη-Fm7dG:dCTP structure was essentially identical to the corresponding undamaged structure, which explained the efficient bypass of the major methylated lesion. Overall, the first structure of translesion synthesis DNA polymerase bypassing Fm7dG suggests that in the catalytic site of Y-family DNA polymerases, small N7-alkylguanine adducts may be well tolerated and form the canonical Watson–Crick base pair with dCTP through their keto tautomers.

Miscoding Properties of 2′-Deoxyinosine, a Nitric Oxide-Derived DNA Adduct, during Translesion Synthesis Catalyzed by Human DNA Polymerases

2008 ◽  
Vol 377 (4) ◽  
pp. 1015-1023 ◽  
Author(s):  
Manabu Yasui ◽  
Emi Suenaga ◽  
Naoki Koyama ◽  
Chikahide Masutani ◽  
Fumio Hanaoka ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Dna Adduct ◽  
Human Dna

scholarly journals Translesion Synthesis Past Equine Estrogen-Derived 2‘-Deoxyadenosine DNA Adducts by Human DNA Polymerases η and κ†

Biochemistry ◽  
2006 ◽  
Vol 45 (19) ◽  
pp. 6187-6194 ◽  
Author(s):  
Manabu Yasui ◽  
Y. R. Santosh Laxmi ◽  
Sreenivasa R. Ananthoju ◽  
Naomi Suzuki ◽  
Sung Yeon Kim ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Dna Polymerases ◽  
Translesion Synthesis ◽  
Human Dna
Sign in / Sign up

Export Citation Format

Share Document