Development of Coconut Shell Activated Carbon-Tethered Urease for Degradation of Urea in a Packed Bed

2013 ◽  
Vol 2 (3) ◽  
pp. 433-439 ◽  
Author(s):  
Lei Wang ◽  
Sha Wang ◽  
Xiangyun Deng ◽  
Yucang Zhang ◽  
Chunrong Xiong
2015 ◽  
Vol 3 (5) ◽  
pp. 509-517 ◽  
Author(s):  
Lei Wang ◽  
Junning Zhang ◽  
Ziting Tan ◽  
Hong Jiang ◽  
Chunrong Xiong

ScienceAsia ◽  
2012 ◽  
Vol 38 (3) ◽  
pp. 283 ◽  
Author(s):  
Achanai Buasri ◽  
Nattawut Chaiyut ◽  
Vorrada Loryuenyong ◽  
Chao Rodklum ◽  
Techit Chaikwan ◽  
...  

2013 ◽  
Vol 295-298 ◽  
pp. 1359-1363
Author(s):  
Lei Wang ◽  
Sha Wang ◽  
Di Xiong ◽  
Chun Rong Xiong

Urease was successfully tethered onto coconut shell activated carbon (AC) granules. Degradation of urea was carried out in packed bed over the tethered urease. For 75% of retained activity, the tethered urease shows a broader temperature range of 42~80°C, compared to 45~75°C for the free enzyme. Similarly, the tethered urease has an increased resistance against the changes of pH. For a relative activity of 80%, the free urease had a pH range of 6.2-7.4, while it was 6.5–8.0 for the tethered urease. The Km values of the free and tethered ureases were 22.60 mg/mL and 7.43 mg/mL, respectively. And the Vm values were actually very close (~23.5 mg/min ) for both the tethered and free ureases. The catalytic performance of the tethered urease was tested in a packed bed reactor. The relative activity was maintained over 80% after 50 h of running in degradation of urea.


2021 ◽  
Vol 6 (34) ◽  
pp. 9149-9156
Author(s):  
Navvamani Rajasekaran ◽  
Mari Vinoba ◽  
Hanadi Al‐Sheeha ◽  
Mohan S. Rana

2019 ◽  
Vol 230 (12) ◽  
Author(s):  
Ksenija Kumrić ◽  
Radojka Vujasin ◽  
Marija Egerić ◽  
Đorđe Petrović ◽  
Aleksandar Devečerski ◽  
...  

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