scholarly journals Analysis of Vaccinia Virus−Host Protein−Protein Interactions: Validations of Yeast Two-Hybrid Screenings

2009 ◽  
Vol 8 (9) ◽  
pp. 4311-4318 ◽  
Author(s):  
Leiliang Zhang ◽  
Nancy Y. Villa ◽  
Masmudur M. Rahman ◽  
Sherin Smallwood ◽  
Donna Shattuck ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Protein Interactions ◽  
Host Protein ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Two Hybrid

scholarly journals A Field-Proven Yeast Two-Hybrid Protocol Used to Identify Coronavirus–Host Protein–Protein Interactions

2015 ◽  
pp. 213-229 ◽  
Author(s):  
Pierre-Olivier Vidalain ◽  
Yves Jacob ◽  
Marne C. Hagemeijer ◽  
Louis M. Jones ◽  
Grégory Neveu ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Host Protein ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Two Hybrid

Analysing protein–protein interactions of the Myxococcus xanthus Dif signalling pathway using the yeast two-hybrid system

Microbiology ◽  
2005 ◽  
Vol 151 (5) ◽  
pp. 1535-1541 ◽  
Author(s):  
Hope L. Lancero ◽  
Schryl Castaneda ◽  
Nora B. Caberoy ◽  
Xiaoyuan Ma ◽  
Anthony G. Garza ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Genomic Library ◽  
Myxococcus Xanthus ◽  
Signalling Pathway ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Fruiting Body Formation ◽  
New Information ◽  
Chemotaxis Proteins ◽  
Two Hybrid

The dif operon is essential for fruiting body formation, fibril (exopolysaccharide) production and social motility of Myxococcus xanthus. The dif locus contains a gene cluster homologous to chemotaxis genes such as mcp (difA), cheW (difC), cheY (difD), cheA (difE) and cheC (difF), as well as an unknown ORF called difB. This study used yeast two-hybrid analysis to investigate possible interactions between Dif proteins, and determined that DifA, C, D and E interact in a similar fashion to chemotaxis proteins of Escherichia coli and Bacillus subtilis. It also showed that DifF interacted with DifD, and that the novel protein DifB did not interact with Dif proteins. Furthermore, DifA–F proteins were used to determine other possible protein–protein interactions in the M. xanthus genomic library. The authors not only confirmed the specific interactions among known Dif proteins, but also discovered two novel interactions between DifE and Nla19, and DifB and YidC, providing some new information about the Dif signalling pathway. Based on these findings, a model for the Dif signalling pathway is proposed.

On the structure of protein–protein interaction networks

2003 ◽  
Vol 31 (6) ◽  
pp. 1491-1496 ◽  
Author(s):  
A. Thomas ◽  
R. Cannings ◽  
N.A.M. Monk ◽  
C. Cannings
Keyword(s):  
Power Law ◽  
Protein Interactions ◽  
Large Scale ◽  
Underlying Structure ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Protein Protein Interaction ◽  
Human Proteins ◽  
Approximate Power ◽  
Two Hybrid

We present a simple model for the underlying structure of protein–protein pairwise interaction graphs that is based on the way in which proteins attach to each other in experiments such as yeast two-hybrid assays. We show that data on the interactions of human proteins lend support to this model. The frequency of the number of connections per protein under this model does not follow a power law, in contrast to the reported behaviour of data from large-scale yeast two-hybrid screens of yeast protein–protein interactions. Sampling sub-graphs from the underlying graphs generated with our model, in a way analogous to the sampling performed in large-scale yeast two-hybrid searches, gives degree distributions that differ subtly from the power law and that fit the observed data better than the power law itself. Our results show that the observation of approximate power law behaviour in a sampled sub-graph does not imply that the underlying graph follows a power law.

Determination of Protein-Protein Interactions of ICln by the Yeast Two-Hybrid System

2001 ◽  
Vol 11 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Andreas Schmarda ◽  
Friedrich Fresser ◽  
Martin Gschwentner ◽  
Johannes Fürst ◽  
Markus Ritter ◽  
...  
Keyword(s):  
Hybrid System ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Yeast Two Hybrid System ◽  
Two Hybrid
Sign in / Sign up

Export Citation Format

Share Document