Proteomic Characteristics ofex vivo-Enriched Adult Human Bone Marrow Mononuclear Cells in Continuous Perfusion Cultures

2009 ◽  
Vol 8 (4) ◽  
pp. 2079-2089 ◽  
Author(s):  
Agasanur K. Prahalad ◽  
Janet M. Hock
Keyword(s):  
Bone Marrow ◽  
Mononuclear Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Mononuclear Cells ◽  
Adult Human ◽  
Perfusion Cultures ◽  
Continuous Perfusion

scholarly journals Arachidonic Acid and Freshly Isolated Human Bone Marrow Mononuclear Cells

1999 ◽  
Vol 8 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Y. Denizot ◽  
V. Desplat ◽  
C. Dulery ◽  
F. Trimoreau ◽  
V. Praloran
Keyword(s):  
Bone Marrow ◽  
Arachidonic Acid ◽  
Fatty Acid ◽  
Mononuclear Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Mononuclear Cells ◽  
Gm Csf ◽  
Chromatography Analysis ◽  
Bone Marrow Plasma

Arachidonic acid (AA), a fatty acid found in the human bone marrow plasma, is the precursor of eicosanoids that modulate bone marrow haematopoiesis. To further our understanding of the role of AA in the bone marrow physiology, we have assessed its incorporation in human bone marrow mononuclear cells. Gas chromatography analysis indicates the presence of AA in their fatty acid composition. In bone marrow mononuclear cells, [3H]-AA is incorporated into triglycerides and is later delivered into phospholipids, a result not observed with blood mononuclear cells. Prelabelling-chase experiments indicate a trafficking of labelled AA from phosphatidylcholine to phosphatidylethanolamine. Stimulation of prelabelled bone marrow mononuclear cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) results in the release of a part of the incorporated labelled AA. Finally, exogenous AA (up to 1 μM) has no significant effect on cell growth. In conclusion, human bone marrow mononuclear cells participate to the control of marrow AA concentrations by incorporating AA into phospholipids and triglycerides. In turn, bone marrow mononuclear cells can release AA in response to the potent haematopoietic growth factor GM-CSF.

Cryopreserved human bone marrow mononuclear cells as a source of mesenchymal stromal cells: application in osteoporosis research

Cytotherapy ◽  
2008 ◽  
Vol 10 (5) ◽  
pp. 460-468 ◽  
Author(s):  
A. Casado-Díaz ◽  
R. Santiago-Mora ◽  
R. Jiménez ◽  
J. Caballero-Villarraso ◽  
C. Herrera ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Mononuclear Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Mononuclear Cells ◽  
Osteoporosis Research

scholarly journals Intracarotid administration of human bone marrow mononuclear cells in rat photothrombotic ischemia

2010 ◽  
Vol 2 (1) ◽  
Author(s):  
Jens Minnerup ◽  
Florian H Seeger ◽  
Katharina Kuhnert ◽  
Kai Diederich ◽  
Matthias Schilling ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mononuclear Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Mononuclear Cells ◽  
Intracarotid Administration

Influence of concentration and preparation of platelet rich fibrin on human bone marrow mononuclear cells (in vitro)

Platelets ◽  
2018 ◽  
Vol 30 (7) ◽  
pp. 861-870 ◽  
Author(s):  
René Verboket ◽  
Carlos Herrera-Vizcaíno ◽  
Kirsten Thorwart ◽  
Patrick Booms ◽  
Marlene Bellen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mononuclear Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Mononuclear Cells ◽  
Platelet Rich Fibrin
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