scholarly journals Approaching Solid Tumor Heterogeneity on a Cellular Basis by Tissue Proteomics Using Laser Capture Microdissection and Biological Mass Spectrometry†

2009 ◽  
Vol 8 (5) ◽  
pp. 2310-2318 ◽  
Author(s):  
Donald J. Johann ◽  
Jaime Rodriguez-Canales ◽  
Sumana Mukherjee ◽  
DaRue A. Prieto ◽  
Jeffrey C. Hanson ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Solid Tumor ◽  
Laser Capture Microdissection ◽  
Tumor Heterogeneity ◽  
Biological Mass Spectrometry ◽  
Cellular Basis ◽  
Tissue Proteomics ◽  
Laser Capture

Proteome-wide analysis of head and neck squamous cell carcinomas using laser-capture microdissection and tandem mass spectrometry

Oral Oncology ◽  
2005 ◽  
Vol 41 (2) ◽  
pp. 183-199 ◽  
Author(s):  
Haven Baker ◽  
Vyomesh Patel ◽  
Alfredo A. Molinolo ◽  
Edward J. Shillitoe ◽  
John F. Ensley ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Head And Neck ◽  
Squamous Cell ◽  
Laser Capture Microdissection ◽  
Squamous Cell Carcinomas ◽  
Tandem Mass ◽  
Laser Capture

scholarly journals Laser capture microdissection in combination with mass spectrometry: Approach to characterization of tissue-specific proteomes of Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)

2020 ◽  
Author(s):  
Pavel Roudnický ◽  
David Potěšil ◽  
Zbyněk Zdráhal ◽  
Milan Gelnar ◽  
Martin Kašný
Keyword(s):  
Mass Spectrometry ◽  
Laser Capture Microdissection ◽  
Bacterial Infections ◽  
Protein Localization ◽  
Model Organism ◽  
Surrounding Tissue ◽  
Laboratory Animals ◽  
Tissue Specific ◽  
Laser Capture ◽  
Eudiplozoon Nipponicum

AbstractEudiplozoon nipponicum (Goto, 1891) is a hematophagous monogenean ectoparasite which inhabits the gills of the common carp (Cyprinus carpio). Heavy infestation can lead to anemia and in conjunction with secondary bacterial infections cause poor health and eventual death of the host.This study is based on an innovative approach to protein localization which has never been used in parasitology before. Using laser capture microdissection, we dissected particular areas of the parasite body without contaminating the samples by surrounding tissue, and in combination with analysis by mass spectrometry obtained tissue-specific proteomes of tegument, intestine, and parenchyma of our model organism, E. nipponicum. We successfully verified the presence of certain functional proteins (e.g. cathepsin L) in tissues where their presence was expected (intestine) and confirmed that there were no traces of these proteins in other tissues (tegument and parenchyma). Additionally, we identified a total of 2,059 proteins, including 72 peptidases and 33 peptidase inhibitors. As expected, the greatest variety was found in the intestine and the lowest variety in the parenchyma.Our results are significant on two levels. Firstly, we demonstrated how one can localize all proteins in one analysis and without using laboratory animals (antibodies for immunolocalization of single proteins). Secondly, this study offers the first complex proteomic data on not only the E. nipponicum but within the whole class of Monogenea, which was from this point of view until recently neglected.

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