scholarly journals Proteomic Analysis of Detergent-Solubilized Membrane Proteins from Insect-Developmental Forms ofTrypanosoma cruzi†

2009 ◽  
Vol 8 (7) ◽  
pp. 3642-3652 ◽  
Author(s):  
Esteban M. Cordero ◽  
Ernesto S. Nakayasu ◽  
Luciana G. Gentil ◽  
Nobuko Yoshida ◽  
Igor C. Almeida ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Proteomic Analysis

Proteomic Analysis of Membrane Proteins fromStreptococcus pneumoniaewith Multiple Separation Methods Plus High Accuracy Mass Spectrometry

2011 ◽  
Vol 15 (10) ◽  
pp. 683-694 ◽  
Author(s):  
Xuesong Sun ◽  
Xiao-Yan Yang ◽  
Xing-Feng Yin ◽  
Guangchuang Yu ◽  
Chuan-Le Xiao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Membrane Proteins ◽  
Proteomic Analysis ◽  
High Accuracy

scholarly journals Quantitative proteomics identifies a plasma multi-protein model for detection of hepatocellular carcinoma

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Zhenhua Du ◽  
Xinyi Liu ◽  
Xiaojun Wei ◽  
Hongbo Luo ◽  
Peiyao Li ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Membrane Proteins ◽  
Early Detection ◽  
Proteomic Analysis ◽  
Quantitative Proteomics ◽  
Serum Biomarkers ◽  
Diagnostic Efficacy ◽  
Protein Model ◽  
Tumor Tissues ◽  
Normal Controls

Abstract More efficient biomarkers are needed to facilitate the early detection of hepatocellular carcinoma (HCC). We aimed to identify candidate biomarkers for HCC detection by proteomic analysis. First, we performed a global proteomic analysis of 10 paired HCC and non-tumor tissues. Then, we validated the top-ranked proteins by targeted proteomic analyses in another tissue cohort. At last, we used enzyme-linked immunosorbent assays to validate the candidate biomarkers in multiple serum cohorts including HCC cases (HCCs), cirrhosis cases (LCs), and normal controls (NCs). We identified and validated 33 up-regulated proteins in HCC tissues. Among them, eight secretory or membrane proteins were further evaluated in serum, revealing that aldo–keto reductase family 1 member B10 (AKR1B10) and cathepsin A (CTSA) can distinguish HCCs from LCs and NCs. The area under the curves (AUCs) were 0.891 and 0.894 for AKR1B10 and CTSA, respectively, greater than that of alpha-fetoprotein (AFP; 0.831). Notably, combining the three proteins reached an AUC of 0.969, which outperformed AFP alone (P < 0.05). Furthermore, the serum AKR1B10 levels dramatically decreased after surgery. AKR1B10 and CTSA are potential serum biomarkers for HCC detection. The combination of AKR1B10, CTSA, and AFP may improve the HCC diagnostic efficacy.

scholarly journals Proteomic Analysis of Membrane Proteins of Vero Cells: Exploration of Potential Proteins Responsible for Virus Entry

2014 ◽  
Vol 33 (1) ◽  
pp. 20-28 ◽  
Author(s):  
Donghua Guo ◽  
Qinghe Zhu ◽  
Hong Zhang ◽  
Dongbo Sun
Keyword(s):  
Membrane Proteins ◽  
Proteomic Analysis ◽  
Virus Entry ◽  
Vero Cells

Proteomic Analysis of Gliosomes from Mouse Brain: Identification and Investigation of Glial Membrane Proteins

2014 ◽  
Vol 13 (12) ◽  
pp. 5918-5927 ◽  
Author(s):  
Karen E. Carney ◽  
Marco Milanese ◽  
Pim van Nierop ◽  
Ka Wan Li ◽  
Stéphane H. R. Oliet ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Mouse Brain ◽  
Proteomic Analysis ◽  
Glial Membrane
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