Proteomic Analysis of a PDEF Ets Transcription Factor-Interacting Protein Complex

2009 ◽  
Vol 8 (3) ◽  
pp. 1327-1337 ◽  
Author(s):  
Je-Yoel Cho ◽  
Minjung Lee ◽  
Jung-Mo Ahn ◽  
Eun-Sung Park ◽  
Ji-Hoon Cho ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Protein Complex ◽  
Proteomic Analysis ◽  
Interacting Protein ◽  
Ets Transcription Factor ◽  
Interacting Protein Complex

A simple coarse-grained model for interacting protein complex

2016 ◽  
Vol 115 (5) ◽  
pp. 587-597 ◽  
Author(s):  
Kazutomo Kawaguchi ◽  
Satoshi Nakagawa ◽  
Shogo Kinoshita ◽  
Makoto Wada ◽  
Hiroaki Saito ◽  
...  
Keyword(s):  
Protein Complex ◽  
Coarse Grained ◽  
Interacting Protein ◽  
Coarse Grained Model ◽  
Interacting Protein Complex

scholarly journals Syntaxin 1A Gene Is Negatively Regulated in a Cell/Tissue Specific Manner by YY1 Transcription Factor, Which Binds to the −183 to −137 Promoter Region Together with Gene Silencing Factors Including Histone Deacetylase

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 146
Author(s):  
Takahiro Nakayama ◽  
Toshiyuki Fukutomi ◽  
Yasuo Terao ◽  
Kimio Akagawa
Keyword(s):  
Transcription Factor ◽  
Gene Silencing ◽  
Protein Complex ◽  
Promoter Region ◽  
Neuronal Cell ◽  
Syntaxin 1A ◽  
Tissue Specific ◽  
Cell Tissue ◽  
Specific Manner ◽  
A Cell

The HPC-1/syntaxin 1A (Stx1a) gene, which is involved in synaptic transmission and neurodevelopmental disorders, is a TATA-less gene with several transcription start sites. It is activated by the binding of Sp1 and acetylated histone H3 to the −204 to +2 core promoter region (CPR) in neuronal cell/tissue. Furthermore, it is depressed by the association of class 1 histone deacetylases (HDACs) to Stx1a–CPR in non-neuronal cell/tissue. To further clarify the factors characterizing Stx1a gene silencing in non-neuronal cell/tissue not expressing Stx1a, we attempted to identify the promoter region forming DNA–protein complex only in non-neuronal cells. Electrophoresis mobility shift assays (EMSA) demonstrated that the −183 to −137 OL2 promoter region forms DNA–protein complex only in non-neuronal fetal rat skin keratinocyte (FRSK) cells which do not express Stx1a. Furthermore, the Yin-Yang 1 (YY1) transcription factor binds to the −183 to −137 promoter region of Stx1a in FRSK cells, as shown by competitive EMSA and supershift assay. Chromatin immunoprecipitation assay revealed that YY1 in vivo associates to Stx1a–CPR in cell/tissue not expressing Stx1a and that trichostatin A treatment in FRSK cells decreases the high-level association of YY1 to Stx1a-CPR in default. Reporter assay indicated that YY1 negatively regulates Stx1a transcription. Finally, mass spectrometry analysis showed that gene silencing factors, including HDAC1, associate onto the −183 to −137 promoter region together with YY1. The current study is the first to report that Stx1a transcription is negatively regulated in a cell/tissue-specific manner by YY1 transcription factor, which binds to the −183 to −137 promoter region together with gene silencing factors, including HDAC.

scholarly journals Dual function of the epithelial specific ets transcription factor, ELF3, in modulating differentiation

Oncogene ◽  
2000 ◽  
Vol 19 (15) ◽  
pp. 1941-1949 ◽  
Author(s):  
Felix H Brembeck ◽  
Oliver G Opitz ◽  
Towia A Libermann ◽  
Anil K Rustgi
Keyword(s):  
Transcription Factor ◽  
Dual Function ◽  
Ets Transcription Factor
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