scholarly journals FLEXIQuant: A Novel Tool for the Absolute Quantification of Proteins, and the Simultaneous Identification and Quantification of Potentially Modified Peptides

2009 ◽  
Vol 8 (5) ◽  
pp. 2201-2210 ◽  
Author(s):  
Sasha Singh ◽  
Michael Springer ◽  
Judith Steen ◽  
Marc W. Kirschner ◽  
Hanno Steen
Keyword(s):  
Absolute Quantification ◽  
Modified Peptides ◽  
The Absolute ◽  
Simultaneous Identification ◽  
Identification And Quantification

scholarly journals Quantification of ionic organo(fluoro)phosphates in decomposed lithium battery electrolytes

RSC Advances ◽  
2017 ◽  
Vol 7 (62) ◽  
pp. 39314-39324 ◽  
Author(s):  
Jennifer Menzel ◽  
Hannah Schultz ◽  
Vadim Kraft ◽  
Juan Pablo Badillo ◽  
Martin Winter ◽  
...  
Keyword(s):  
Lithium Battery ◽  
Simultaneous Identification ◽  
Identification And Quantification

Simultaneous identification and quantification of organofluorophosphates via the developed setup.

scholarly journals Development and Validation of a Reversed Phase High Performance Liquid Chromatography-Photodiode Array Detection Method for Simultaneous Identification and Quantification of Coumarin, Precocene-I, β-Caryophyllene Oxide, α-Humulene, and β-Caryophyllene in Ageratum Conyzoides Extracts and Essential Oils from Plants

2020 ◽  
Vol 103 (3) ◽  
pp. 857-864
Author(s):  
Sonal Shah ◽  
Tushar Dhanani ◽  
Sonu Sharma ◽  
Raghuraj Singh ◽  
Satyanshu Kumar ◽  
...  
Keyword(s):  
Essential Oils ◽  
Detection Method ◽  
Reversed Phase ◽  
Caryophyllene Oxide ◽  
Ageratum Conyzoides ◽  
Photodiode Array Detection ◽  
Photodiode Array ◽  
Development And Validation ◽  
Simultaneous Identification ◽  
Identification And Quantification

Abstract Background Ageratum conyzoides is an aromatic plant. It is considered as an invasive and cosmopolite weed, widely spread in tropical and subtropical regions. Phytochemicals such as benzopyrenes, flavonoids, and terpenoids are reported from A. conyzoides. Objective Development and validation of a reversed-phase HPLC-photodiode array (PDA) detection method for simultaneous identification and quantification of coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β-caryophyllene in extracts of A. conyzoides and essential oils was carried out. Methods Separation of analytes was achieved on a RP-18 (250 mm × 4.6 mm, 5 µm) column using a solvent system comprising of a mixture of acetonitrile and water with 0.05% trifluoroacetic acid in gradient elution mode at ambient temperature with flow rate of 1 mL/min. Results The retention time of coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β-caryophyllene was 4.38, 12.86, 20.10, 33.34, and 35.11 min, respectively. Limits of detection for coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β-caryophyllene were 2.5, 2.5, 2.5, 0.025, and 2.5 µg/mL, respectively. Similarly, LOQ were 10, 10, 10, 0.10, and 10 µg/mL for coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β- caryophyllene, respectively. Repeatabilities (RSD, %) values for intraday and interday precision for coumarin, precocene-I, β-caryophyllene oxide, α-humulene, and β-caryophyllene was 0.765–2.086 and 0.886–2.128; 0.879–1.672 and 0.979–1.825; 0.696–2.418 and 0.768–2.592; 1.728–2.362 and 1.965–2.378; 1.615–2.897 and 1.658–2.906, respectively. Conclusions The separation of five analytes was achieved within 50 min. The developed and validated HPLC-PDA method was successfully applied for identification and quantification of above five analytes in A. conyzoides extracts and essential oils. The method could be used for meeting the characterization criteria of phytoformulations.

A technique for the absolute quantification of 131I radiopharmaceuticals using SPECT

1993 ◽  
Vol 14 (6) ◽  
pp. 498-505???505 ◽  
Author(s):  
J. S. FLEMING ◽  
A. A. ALAAMER ◽  
S. PERRING
Keyword(s):  
Absolute Quantification ◽  
The Absolute
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