Comparative Proteomics of Tuber Induction, Development and Maturation Reveal the Complexity of Tuberization Process in Potato (Solanum tuberosumL.)

2008 ◽  
Vol 7 (9) ◽  
pp. 3803-3817 ◽  
Author(s):  
Lalit Agrawal ◽  
Subhra Chakraborty ◽  
Dinesh Kumar Jaiswal ◽  
Sonika Gupta ◽  
Asis Datta ◽  
...  
Keyword(s):  
Comparative Proteomics ◽  
Tuber Induction

Comparative Proteomics and Bioinformatics Analysis of Tissue from Non-Small Cell Lung Cancer Patients

2017 ◽  
Vol 14 (1) ◽  
pp. 58-77
Author(s):  
Sevgi Gezici ◽  
Mehmet Ozaslan ◽  
Gurler Akpinar ◽  
Murat Kasap ◽  
Maruf Sanli ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cancer Patients ◽  
Cell Lung Cancer ◽  
Bioinformatics Analysis ◽  
Comparative Proteomics ◽  
Small Cell ◽  
Small Cell Lung ◽  
Lung Cancer Patients

scholarly journals Comparative Proteomics of Marinobacter sp. TT1 Reveals Corexit Impacts on Hydrocarbon Metabolism, Chemotactic Motility, and Biofilm Formation

2020 ◽  
Vol 9 (1) ◽  
pp. 3
Author(s):  
Saskia Rughöft ◽  
Nico Jehmlich ◽  
Tony Gutierrez ◽  
Sara Kleindienst
Keyword(s):  
Biofilm Formation ◽  
Oil Spills ◽  
Carbon Sources ◽  
Comparative Proteomics ◽  
Hydrocarbon Biodegradation ◽  
Solvent Tolerance ◽  
Marine Microorganisms ◽  
Degrading Bacteria ◽  
Oil Spill Response ◽  
First Time

The application of chemical dispersants during marine oil spills can affect the community composition and activity of marine microorganisms. Several studies have indicated that certain marine hydrocarbon-degrading bacteria, such as Marinobacter spp., can be inhibited by chemical dispersants, resulting in lower abundances and/or reduced biodegradation rates. However, a major knowledge gap exists regarding the mechanisms underlying these physiological effects. Here, we performed comparative proteomics of the Deepwater Horizon isolate Marinobacter sp. TT1 grown under different conditions. Strain TT1 received different carbon sources (pyruvate vs. n-hexadecane) with and without added dispersant (Corexit EC9500A). Additional treatments contained crude oil in the form of a water-accommodated fraction (WAF) or chemically-enhanced WAF (CEWAF; with Corexit). For the first time, we identified the proteins associated with alkane metabolism and alginate biosynthesis in strain TT1, report on its potential for aromatic hydrocarbon biodegradation and present a protein-based proposed metabolism of Corexit components as carbon substrates. Our findings revealed that Corexit exposure affects hydrocarbon metabolism, chemotactic motility, biofilm formation, and induces solvent tolerance mechanisms, like efflux pumps, in strain TT1. This study provides novel insights into dispersant impacts on microbial hydrocarbon degraders that should be taken into consideration for future oil spill response actions.

scholarly journals Unveiling Putative Functions of Mucus Proteins and Their Tryptic Peptides in Seven Gastropod Species Using Comparative Proteomics and Machine Learning-Based Bioinformatics Predictions

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3475
Author(s):  
Viroj Tachapuripunya ◽  
Sittiruk Roytrakul ◽  
Pramote Chumnanpuen ◽  
Teerasak E-kobon
Keyword(s):  
Bioactive Peptides ◽  
Nearest Neighbor ◽  
Peptide Identification ◽  
Comparative Proteomics ◽  
Mass Spectrometric ◽  
Functional Categories ◽  
K Nearest Neighbor ◽  
Gastropod Species ◽  
Bioactive Properties ◽  
Sds Page

Gastropods are among the most diverse animals. Gastropod mucus contains several glycoproteins and peptides that vary by species and habitat. Some bioactive peptides from gastropod mucus were identified only in a few species. Therefore, using biochemical, mass spectrometric, and bioinformatics approaches, this study aimed to comprehensively identify putative bioactive peptides from the mucus proteomes of seven commonly found or commercially valuable gastropods. The mucus was collected in triplicate samples, and the proteins were separated by 1D-SDS-PAGE before tryptic digestion and peptide identification by nano LC-MS/MS. The mucus peptides were subsequently compared with R scripts. A total of 2818 different peptides constituting 1634 proteins from the mucus samples were identified, and 1218 of these peptides (43%) were core peptides found in the mucus of all examined species. Clustering and correspondence analyses of 1600 variable peptides showed unique mucous peptide patterns for each species. The high-throughput k-nearest neighbor and random forest-based prediction programs were developed with more than 95% averaged accuracy and could identify 11 functional categories of putative bioactive peptides and 268 peptides (9.5%) with at least five to seven bioactive properties. Antihypertensive, drug-delivering, and antiparasitic peptides were predominant. These peptides provide an understanding of gastropod mucus, and the putative bioactive peptides are expected to be experimentally validated for further medical, pharmaceutical, and cosmetic applications.

scholarly journals Comparative Proteomics Reveals Novel Components at the Plasma Membrane of Differentiated HepaRG Cells and Different Distribution in Hepatocyte- and Biliary-Like Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e71859 ◽  
Author(s):  
Catalina Petrareanu ◽  
Alina Macovei ◽  
Izabela Sokolowska ◽  
Alisa G. Woods ◽  
Catalin Lazar ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Comparative Proteomics ◽  
Heparg Cells
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