Multiplexed MRM with Internal Standards for Cerebrospinal Fluid Candidate Protein Biomarker Quantitation

2014 ◽  
Vol 13 (8) ◽  
pp. 3733-3747 ◽  
Author(s):  
Andrew J. Percy ◽  
Juncong Yang ◽  
Andrew G. Chambers ◽  
Romain Simon ◽  
Darryl B. Hardie ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Internal Standards ◽  
Protein Biomarker ◽  
Candidate Protein ◽  
Biomarker Quantitation

scholarly journals Cerebrospinal fluid extracellular vesicle enrichment for protein biomarker discovery in neurological disease; multiple sclerosis

2017 ◽  
Vol 6 (1) ◽  
pp. 1369805 ◽  
Author(s):  
Joanne L. Welton ◽  
Samantha Loveless ◽  
Timothy Stone ◽  
Chris von Ruhland ◽  
Neil P. Robertson ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Neurological Disease ◽  
Biomarker Discovery ◽  
Extracellular Vesicle ◽  
Protein Biomarker

scholarly journals Protein variability in cerebrospinal fluid and its possible implications for neurological protein biomarker research

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0206478 ◽  
Author(s):  
Lukas M. Schilde ◽  
Steffen Kösters ◽  
Simone Steinbach ◽  
Karin Schork ◽  
Martin Eisenacher ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Protein Biomarker ◽  
Biomarker Research

Cerebrospinal fluid markers of idiopathic intracranial hypertension: Is the renin-angiotensinogen system involved?

Cephalalgia ◽  
2010 ◽  
Vol 31 (1) ◽  
pp. 116-121 ◽  
Author(s):  
Johannes Brettschneider ◽  
Nele Hartmann ◽  
Vera Lehmensiek ◽  
Helga Mogel ◽  
Albert C Ludolph ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Intracranial Hypertension ◽  
Idiopathic Intracranial Hypertension ◽  
Binding Protein ◽  
Regulatory Element ◽  
Renin Angiotensin System ◽  
Element Binding Protein ◽  
Sterol Regulatory Element ◽  
Candidate Protein ◽  
Alpha 1 Antitrypsin

Background: The causes underlying idiopathic intracranial hypertension (IIH) are poorly understood. Methods: To identify disease-related biomarkers that could offer a new insight into IIH pathology, we analyzed the cerebrospinal fluid (CSF) of 18 patients with IIH and 18 controls using two-dimensional fluorescence differential in-gel electrophoresis (2-D DIGE). Results: We found six proteins that were upregulated in IIH (sterol regulatory element-binding protein 1, zinc-alpha-2-glycoprotein, immunoglobulin heavy constant alpha 1 [IGHA1], alpha-1-antitrypsin [SERPINA1], serotransferrin, haptoglobin) and four proteins that were downregulated (hemopexin, angiotensinogen, vitamin-D-binding protein, transthyretin). The validity of our approach was confirmed for one candidate protein (angiotensinogen). To account for a dependency from blood-CSF barrier function, the ratio of angiotensinogen and albumin CSF-to-serum quotients (Qang/Qalb) was determined, which confirmed the downregulation of angiotensinogen in IIH ( p = .04). Conclusion: Previous studies showed the intrinsic renin-angiotensin system (RAS) to regulate choroid plexus blood flow and CSF production. Altered levels of angiotensinogen could indicate an imbalance of the RAS in IIH that may provide new targets for therapeutic intervention.

Time-dependent changes of protein biomarker levels in the cerebrospinal fluid after blast traumatic brain injury

2012 ◽  
Vol 33 (24) ◽  
pp. 3705-3711 ◽  
Author(s):  
Farid Ahmed ◽  
Andrea Gyorgy ◽  
Alaa Kamnaksh ◽  
Geoffrey Ling ◽  
Lawrence Tong ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Cerebrospinal Fluid ◽  
Brain Injury ◽  
Time Dependent ◽  
Protein Biomarker ◽  
Blast Traumatic Brain Injury

scholarly journals Blood and CSF Biomarker Dynamics in Multiple Sclerosis: Implications for Data Interpretation

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
M. J. Eikelenboom ◽  
B. M. J. Uitdehaag ◽  
A. Petzold
Keyword(s):  
Multiple Sclerosis ◽  
Central Nervous System ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Peripheral Nervous System ◽  
Serum Levels ◽  
Disability Progression ◽  
Protein Biomarker ◽  
The Central Nervous System ◽  
Neuroaxonal Degeneration

Background. Disability in multiple sclerosis (MS) is related to neuroaxonal degeneration. A reliable blood biomarker for neuroaxonal degeneration is needed.Objectives. To explore the relationship between cerebrospinal fluid (CSF) and serum concentrations of a protein biomarker for neuroaxonal degeneration, the neurofilaments heavy chain (NfH).Methods. An exploratory cross-sectional (n=51) and longitudinal (n=34) study on cerebrospinal fluid (CSF) and serum NfH phosphoform levels in patients with MS. The expanded disability status scale (EDSS), CSF, and serum levels of NfH-SMI34 and NfH-SMI35 were quantified at baseline. Disability progression was assessed at 3-year followup.Results. At baseline, patients with primary progressive MS (PPMS, EDSS 6) and secondary progressive MS (SPMS, EDSS 6) were more disabled compared to patients with relapsing remitting MS (RRMS, EDSS 2,P<.0001). Serum and CSF NfH phosphoform levels were not correlated. Baseline serum levels of the NfH-SMI34 were significantly (P<.05) higher in patients with PPMS (2.05 ng/mL) compared to SPMS (0.03 ng/mL) and RRMS (1.56 ng/mL). In SPMS higher serum than CSF NfH-SMI34 levels predicted disability progression from baseline (ΔEDSS2,P<.05). In RRMS higher CSF than serum NfH-SMI35 levels predicted disability progression (ΔEDSS2,P<.05).Conclusion. Serum and CSF NfH-SMI34 and NfH-SMI35 levels did not correlate with each other in MS. The quantitative relationship of CSF and serum NfH levels suggests that neuroaxonal degeneration of the central nervous system is the likely cause for disability progression in RRMS. In more severely disabled patients with PP/SPMS, subtle pathology of the peripheral nervous system cannot be excluded as an alternative source for blood NfH levels. Therefore, the interpretation of blood protein biomarker data in diseases of the central nervous system (CNS) should consider the possibility that pathology of the peripheral nervous system (PNS) may influence the results.

scholarly journals Cerebrospinal Fluid Protein Biomarker Panel for Assessment of Neurotoxicity Induced by Kainic Acid in Rats

2012 ◽  
Vol 130 (1) ◽  
pp. 158-167 ◽  
Author(s):  
Olena Y. Glushakova ◽  
Andreas Jeromin ◽  
Juan Martinez ◽  
Danny Johnson ◽  
Nancy Denslow ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Kainic Acid ◽  
Protein Biomarker ◽  
Biomarker Panel ◽  
Cerebrospinal Fluid Protein

Advances in multiplexed MRM-based protein biomarker quantitation toward clinical utility

2014 ◽  
Vol 1844 (5) ◽  
pp. 917-926 ◽  
Author(s):  
Andrew J. Percy ◽  
Andrew G. Chambers ◽  
Juncong Yang ◽  
Darryl B. Hardie ◽  
Christoph H. Borchers
Keyword(s):  
Clinical Utility ◽  
Protein Biomarker ◽  
Biomarker Quantitation
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