Comprehensive Analysis of Protein N-Glycosylation Sites by Combining Chemical Deglycosylation with LC–MS

2014 ◽  
Vol 13 (3) ◽  
pp. 1466-1473 ◽  
Author(s):  
Weixuan Chen ◽  
Johanna M. Smeekens ◽  
Ronghu Wu
Keyword(s):  
Comprehensive Analysis ◽  
Glycosylation Sites ◽  
Chemical Deglycosylation

scholarly journals Precise, Fast and Comprehensive Analysis of Intact Glycopeptides and Monosaccharide-Modifications with pGlyco3

2021 ◽  
Author(s):  
Wen-Feng Zeng ◽  
Wei-Qian Cao ◽  
Ming-Qi Liu ◽  
Si-Min He ◽  
Peng-Yuan Yang
Keyword(s):  
Search Engine ◽  
Software Tools ◽  
Comprehensive Analysis ◽  
The Other ◽  
Identification Accuracy ◽  
Superior Performance ◽  
Isotopic Labelling ◽  
Link Type ◽  
Glycosylation Sites ◽  
Search Speed

AbstractWe presented a glycan-first glycopeptide search engine, pGlyco3, to comprehensively analyze intact N- and O-glycopeptides, including glycopeptides with monosaccharide-modifications. We developed an algorithm, termed pGlycoSite, to localize the glycosylation sites and estimate the localization probabilities. We designed a number of experiments to validate the accuracy of pGlyco3 as well as other frequently used or recently developed software tools. These experiments showed that pGlyco3 outperformed the other tools on both N- and O-glycopeptide identification accuracy especially at the glycan level, without loss of the sensitivity. pGlyco3 also achieved a superior performance in terms of search speed. As pGlyco3 was shown to be accurate and flexible for glycopeptide search with monosaccharide-modifications, we then discovered a monosaccharide-modification of Hex (or an uncommon monosaccharide) “Hex+17.027 Da” on both O-mannose and N-glycopeptides in yeast samples, and confirmed this monosaccharide based on released N-glycans and isotopic labelling data. pGlyco3 is freely available on https://github.com/pFindStudio/pGlyco3/releases.

scholarly journals Targeted Mutants of Cochliobolus carbonum Lacking the Two Major Extracellular Polygalacturonases

1998 ◽  
Vol 64 (4) ◽  
pp. 1497-1503 ◽  
Author(s):  
John S. Scott-Craig ◽  
Yi-Qiang Cheng ◽  
Felice Cervone ◽  
Giulia De Lorenzo ◽  
John W. Pitkin ◽  
...  
Keyword(s):  
Molecular Mass ◽  
Residual Activity ◽  
Pectin Methylesterase ◽  
Pcr Primers ◽  
Gene Replacement ◽  
Amino Acid Identity ◽  
Cochliobolus Carbonum ◽  
Glycosylation Sites ◽  
Mutant Strains ◽  
Chemical Deglycosylation

ABSTRACT The filamentous fungus Cochliobolus carbonum produces endo-α1,4-polygalacturonase (endoPG), exo-α1,4-polygalacturonase (exoPG), and pectin methylesterase when grown in culture on pectin. Residual activity in a pgn1 mutant (lacking endoPG) was due to exoPG activity, and the responsible protein has now been purified. After chemical deglycosylation, the molecular mass of the purified protein decreased from greater than 60 to 45 kDa. The gene that encodes exoPG, PGX1, was isolated with PCR primers based on peptide sequences from the protein. The product of PGX1, Pgx1p, has a predicted molecular mass of 48 kDa, 12 potential N-glycosylation sites, and 61% amino acid identity to an exoPG from the saprophytic fungus Aspergillus tubingensis. Strains of C. carbonum mutated in PGX1 were constructed by targeted gene disruption and by gene replacement. Growth of pgx1mutant strains on pectin was reduced by ca. 20%, and they were still pathogenic on maize. A double pgn1/pgx1 mutant strain was constructed by crossing. The double mutant grew as well as thepgx1 single mutant on pectin and was still pathogenic despite having less than 1% of total wild-type PG activity. Double mutants retained a small amount of PG activity with the same cation-exchange retention time as Pgn1p and also pectin methylesterase and a PG activity associated with the mycelium. Continued growth of thepgn1/pgx1 mutant on pectin could be due to one or more of these residual activities.

Structural Aspects on the Glycosylation Sites in Human Fibrinogen

1979 ◽  
Vol 41 (04) ◽  
pp. 671-676 ◽  
Author(s):  
Edda Töpfer-Petersen ◽  
Friedrich Lottspeich ◽  
Agnes Henschen
Keyword(s):  
Human Fibrinogen ◽  
Glycosylation Sites ◽  
Structural Aspects

Sozial-räumliche Dynamiken der Agrartreibstoffe.

2016 ◽  
Vol 46 (184) ◽  
pp. 423-440 ◽  
Author(s):  
Kristina Dietz ◽  
Bettina Engels ◽  
Oliver Pye
Keyword(s):  
Political Economy ◽  
Capital Flows ◽  
Spatial Dynamics ◽  
Financial Sector ◽  
Comprehensive Analysis ◽  
Spatial Theory ◽  
Global Political Economy

This article explores the spatial dynamics of agrofuels. Building on categories from the field of critical spatial theory, it shows how these categories enable a comprehensive analysis of the spatial dynamics of agrofuels that links the macro-structures of the global political economy to concrete, place-based struggles. Four core socio-spatial dynamics of agrofuel politics are highlighted and applied to empirical findings: territorialization, the financial sector as a new scale of regulation, place-based struggles and transnational spaces of resources and capital flows.

Concerning the abuse of power by an official

2020 ◽  
pp. 103-110
Author(s):  
Ihor Oheruk
Keyword(s):  
Criminal Code ◽  
Comprehensive Analysis ◽  
Practical Significance ◽  
Abuse Of Power ◽  
Government Functions ◽  
Analysis And Synthesis ◽  
The Status ◽  
The Subject ◽  
Criminal Legislation ◽  
Logical Semantic

Purpose. The purpose of the work is to analyze the application of the second and third parts of Article 3692 of the Criminal Code of Ukraine to officials in the context, that defines them by the Criminal Code of Ukraine in the note to Article 364 of the Criminal Code of Ukraine. Methodology. The methodology includes a comprehensive analysis and synthesis of the available scientific and theoretical material and the formulation of relevant conclusions and recommendations. In the course of the study, the following methods of scientific knowledge were used: terminological, logical-semantic, system-structural, logical-normative. Results: in the course of research the cause of criminalization of such act as "abuse of power" is considered, the subject of the specified criminal act which has the features of "an official" in the context, that defines it by the note to Article 364 of the Criminal Code of Ukraine is analyzed and the main ways of committing criminal acts, that are provided for in this article of the Criminal Code of Ukraine are identified. Originality. The study found, that one of the key conditions for the opportunity to influence officials, that are authorized to perform government or local self-government functions, is the position held by the official and the related opportunities. Therefore, taking into account the opinion of the scientists, that the subject of crimes, that are provided for by the second and third parts of Article 3692 is special, the peculiarities of which is the cumulative feature, that denotes, that such person is not endowed with the status of an official, well-founded need to specify the criminal legislation of Ukraine in terms of the application the second and third parts of Article 3692 of the Criminal code of Ukraine concerning officials in the context, that defines them by the criminal legislation of Ukraine in the note to Article 364 of the Criminal Code of Ukraine. Practical significance. The research results can be used in lawmaking in the improvement of anti-corruption legislation.

BASIC FUNDAMENTALS OF CIVIL LAW AND THEIR IMPORTANCE

2019 ◽  
pp. 120-126
Author(s):  
Diana Vivcharuk
Keyword(s):  
Law Enforcement ◽  
Scientific Knowledge ◽  
Civil Law ◽  
Comprehensive Analysis ◽  
Practical Significance

Purpose. The purpose of the article is the regulation of relations on the principles of civil law. Methodology. The methodology includes a comprehensive analysis and a synthesis of available scientific and theoretical information. It is includes the formulation of relevant conclusions and recommendations. Such methods of scientific knowledge were used: terminological, functional, systemic-structural, logical-normative. Results: it was determined, that principles of civil law – an ideas of the civil law, that characterized by systematic,versatile, more stable, more regylated. Originality. An article is the special reseach that explores the problems of civil law in Ukraine. Practical significance. The results of the research can be used in legislation and law-enforcement activities.

AKAD FINANCING OF MURABAHAH BETWEEN CUSTOMERS WITH MUAMALAT BANKS HARKAT VIEWED FROM SHARI'AH PRINCIPLES

2020 ◽  
Vol 10 (1) ◽  
pp. 13-26
Author(s):  
Candra Irawan ◽  
Adi Bastian ◽  
Febrozi Rohadi
Keyword(s):  
Data Collection ◽  
Financial Institutions ◽  
Research Method ◽  
Economic Condition ◽  
Field Research ◽  
Comprehensive Analysis ◽  
Islamic Banks ◽  
Legal Research ◽  
Trading System ◽  
Library Research

Currently in Indonesia Islamic Bank has gained a place and interested in the community, causing many emerging Syari'ah Bank and Financial Institutions of the syari'ah, and products in Islamic banks are widely used is murabahah financing. The formulation of the problem in this research are: (1). How is the implementation of the sale and purchase through murabahah financing between Bank Muamalat Harkat with customers. (2). Is trading system murabahah financing between Bank Muamalat Harkat and customers have been according to the principles of Syari’ah. (3). How murabahah financing efforts to resolve the breach between the customer and Muamalat Harkat. This research method is empirical legal research, this study was conducted in Bank Muamalat Harkat based data collection through field research such as interviews, observation and description as well as information from respondents through library research. The results of this research are: before an agreement Bank to assess carefully the prospective customer in the form of a comprehensive analysis and is divided into several stages, such as the assessment using the principle of 5C Character (Character of the debitor), Chapacity (Capability Candidate Debitor) , Capital (Capital candidate Debitor), Collateral (Collateral candidate Debitor) and Condition of economy (economic condition of the prospective Borrower). Trading system murabahah financing between Bank Muamalat Harkat with the customer has not fully based on the principles of the Syari'ah. Murabahah financing efforts to resolve the defaults can be solved by R3 is Restrukturing (Arrangement Back), Reconditioning (Terms Back) and Rescheduling (rescheduling), sales collateral and auction execution. 

Concerning the Concept of Light-Colour Arrangement of the Urban Environment in the Central Part of Tyumen

2020 ◽  
pp. 34-42
Author(s):  
Nikolay I. Shchepetkov ◽  
Svetlana B. Kapeleva ◽  
Denis V. Bugaev ◽  
Gregory S. Matovnikov ◽  
Anna S. Kostareva
Keyword(s):  
Urban Environment ◽  
Comprehensive Analysis ◽  
Assessment Criteria ◽  
Field Observations ◽  
General Plan ◽  
Design Solutions ◽  
Methodological Principles ◽  
The City

The article provides a comprehensive analysis of outdoor lighting in the central part of Tyumen (with consideration of conducted field observations) and prospects of its development on the basis of the general plan of illumination of the central part of the city being under design. Main provisions of this general plan as well as methodological principles and assessment criteria of design solutions illustrat-ed by photographs, schemes and visualisations of the illuminated objects are described.

Evaluation of epidemiological data on effect of therapy with Ipigrix® on motor and sensory functions in ambulatory patients with various diseases ofperipheral nervous system

2019 ◽  
Vol 1 (2) ◽  
pp. 11-14
Author(s):  
O. S. Levin ◽  
O. V. Matvievskaya
Keyword(s):  
Nervous System ◽  
Observational Study ◽  
Peripheral Nervous System ◽  
Epidemiological Data ◽  
Comprehensive Analysis ◽  
Ambulatory Patients ◽  
Effect Of Therapy ◽  
Sensory Functions

The article contains a comprehensive analysis of the summary epidemiological data obtained during the observational study to assess the effect of therapy with Ipigrix® on the dynamics of motor and sensory functions, as well as the severity of pain in outpatient patients with various diseases of the peripheral nervous system: mononeuropathy, polyneuropathy and polyradiculopathy of various origins.

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