scholarly journals High-Affinity Recombinant Antibody Fragments (Fabs) Can Be Applied in Peptide Enrichment Immuno-MRM Assays

2014 ◽  
Vol 13 (4) ◽  
pp. 2187-2196 ◽  
Author(s):  
Jeffrey R. Whiteaker ◽  
Lei Zhao ◽  
Christian Frisch ◽  
Francisco Ylera ◽  
Stefan Harth ◽  
...  
Keyword(s):  
Recombinant Antibody ◽  
Antibody Fragments ◽  
High Affinity ◽  
Recombinant Antibody Fragments ◽  
Peptide Enrichment

scholarly journals Generation of High-Affinity Chicken Single-Chain Fv Antibody Fragments for Measurement of the Pseudonitzschia pungens Toxin Domoic Acid

2006 ◽  
Vol 72 (5) ◽  
pp. 3343-3349 ◽  
Author(s):  
William J. J. Finlay ◽  
Iain Shaw ◽  
Joanna P. Reilly ◽  
Marian Kane
Keyword(s):  
Domoic Acid ◽  
Recombinant Antibody ◽  
Variable Region ◽  
Antibody Fragments ◽  
Enzyme Linked Immunosorbent Assay ◽  
Single Chain ◽  
Single Chain Antibody ◽  
High Affinity ◽  
Single Chain Fv ◽  
Recombinant Antibody Fragments

ABSTRACT Antibody-based assay systems are now accepted by regulatory authorities for detection of the toxins produced by phytoplankton that accumulate in shellfish tissues. However, the generation of suitable antibodies for sensitive assay development remains a major challenge. We have examined the potential of using the chicken immune system to generate high-affinity, high-specificity recombinant antibody fragments against phytotoxins. Following immunization of the chicken with domoic acid-bovine serum albumin, a single-chain antibody variable region (scFv) gene library was generated from single VH and VL genes isolated from the immune cells in the spleen and bone marrow. scFvs reacting with domoic acid were isolated by phage display and affinity matured by light chain shuffling, resulting in an approximate 10-fold increase in sensitivity. The isolated scFvs were effectively expressed in Escherichia coli and readily purified by affinity chromatography. They were then used to develop a convenient and sensitive indirect competitive enzyme-linked immunosorbent assay for domoic acid, with a 50% effective dose of 156 ng/ml, which could be used reliably with shellfish extracts. This study demonstrates that chickens provide a valuable model system for the simplified, rapid generation of high-affinity recombinant antibody fragments with specificity for small toxin molecules.

The venom of the scorpion Centruroides limpidus, which causes the highest number of stings in Mexico, is neutralized by two recombinant antibody fragments

2021 ◽  
Vol 137 ◽  
pp. 247-255
Author(s):  
Guillermo Fernández-Taboada ◽  
Lidia Riaño-Umbarila ◽  
Alejandro Olvera-Rodríguez ◽  
Ilse Viridiana Gómez-Ramírez ◽  
Luis Fernando Losoya-Uribe ◽  
...  
Keyword(s):  
Recombinant Antibody ◽  
Antibody Fragments ◽  
Recombinant Antibody Fragments

Isolation and characterisation of Ebolavirus-specific recombinant antibody fragments from murine and shark immune libraries

2011 ◽  
Vol 48 (15-16) ◽  
pp. 2027-2037 ◽  
Author(s):  
Sarah A. Goodchild ◽  
Helen Dooley ◽  
Randal J. Schoepp ◽  
Martin Flajnik ◽  
Stephen G. Lonsdale
Keyword(s):  
Recombinant Antibody ◽  
Antibody Fragments ◽  
Recombinant Antibody Fragments

Seeing Better through a MIST:  Evaluation of Monoclonal Recombinant Antibody Fragments on Microarrays

2004 ◽  
Vol 76 (10) ◽  
pp. 2916-2921 ◽  
Author(s):  
Philipp Angenendt ◽  
Jeannine Wilde ◽  
Gregor Kijanka ◽  
Sabine Baars ◽  
Dolores J. Cahill ◽  
...  
Keyword(s):  
Recombinant Antibody ◽  
Antibody Fragments ◽  
Recombinant Antibody Fragments
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