Influenza A Infection of Primary Human Airway Epithelial Cells Up-Regulates Proteins Related to Purine Metabolism and Ubiquitin-Related Signaling

2013 ◽  
Vol 12 (7) ◽  
pp. 3139-3151 ◽  
Author(s):  
Andrea L. Kroeker ◽  
Peyman Ezzati ◽  
Kevin M. Coombs ◽  
Andrew J. Halayko
Keyword(s):  
Epithelial Cells ◽  
Influenza A ◽  
Airway Epithelial Cells ◽  
Purine Metabolism ◽  
Airway Epithelial ◽  
Human Airway ◽  
Human Airway Epithelial Cells

Infection of cultured human airway epithelial cells by influenza a virus

Life Sciences ◽  
1991 ◽  
Vol 49 (16) ◽  
pp. 1173-1181 ◽  
Author(s):  
Theodore F. Reiss ◽  
Dieter C. Gruenert ◽  
Jay A. Nadel ◽  
David B. Jacoby
Keyword(s):  
Epithelial Cells ◽  
Influenza A Virus ◽  
Influenza A ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Human Airway ◽  
Human Airway Epithelial Cells

scholarly journals Adaptation of influenza A (H7N9) virus in primary human airway epithelial cells

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Daniel Tsung-Ning Huang ◽  
Chun-Yi Lu ◽  
Ya-Hui Chi ◽  
Wan-Ling Li ◽  
Luan-Yin Chang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Influenza A ◽  
Airway Epithelial Cells ◽  
H7n9 Virus ◽  
Airway Epithelial ◽  
Human Airway ◽  
Human Airway Epithelial Cells

scholarly journals Cation currents in human airway epithelial cells induced by infection with influenza A virus

2009 ◽  
Vol 587 (13) ◽  
pp. 3159-3173 ◽  
Author(s):  
M. Gallacher ◽  
S. G. Brown ◽  
B. G. Hale ◽  
R. Fearns ◽  
R. E. Olver ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Influenza A Virus ◽  
Influenza A ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Human Airway ◽  
Human Airway Epithelial Cells

scholarly journals IFITM3 Clusters on Virus Containing Endosomes and Lysosomes Early in the Influenza A Infection of Human Airway Epithelial Cells

Viruses ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 548 ◽  
Author(s):  
Susann Kummer ◽  
Ori Avinoam ◽  
Hans-Georg Kräusslich
Keyword(s):  
Epithelial Cells ◽  
Influenza A ◽  
Airway Epithelial Cells ◽  
Epithelial Cell Line ◽  
Airway Epithelial ◽  
Human Lung Epithelial Cell ◽  
Human Airway ◽  
Lung Epithelial ◽  
Endosomal Vesicles ◽  
Human Airway Epithelial Cells

Interferon-induced transmembrane proteins (IFITMs) have been shown to strongly affect influenza A virus (IAV) infectivity in tissue culture. Moreover, polymorphisms in IFITM3 have been associated with the severity of the disease in humans. IFITM3 appears to act early in the infection, but its mechanism of action and potential interactions with incoming IAV structures are not yet defined. Here, we visualized endogenous IFITM3 interactions with IAV in the human lung epithelial cell line A549 and in primary human airway epithelial cells employing stimulated emission depletion super-resolution microscopy. By applying an iterative approach for the cluster definition and computational cluster analysis, we found that IFITM3 reorganizes into clusters as IAV infection progresses. IFITM3 cluster formation started at 2-3 h post infection and increased over time to finally coat IAV-containing endosomal vesicles. This IAV-induced phenotype was due to the endosomal recruitment of IFITM3 rather than to an overall increase in the IFITM3 abundance. While the IAV-induced IFITM3 clustering and localization to endosomal vesicles was comparable in primary human airway epithelial cells and the human lung epithelial cell line A549, the endogenous IFITM3 signal was higher in primary cells. Moreover, we observed IFITM3 signals adjacent to IAV-containing recycling endosomes.

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