Comparative Proteomics of Primary and Secondary Lutoids Reveals that Chitinase and Glucanase Play a Crucial Combined Role in Rubber Particle Aggregation in Hevea brasiliensis

2013 ◽  
Vol 12 (11) ◽  
pp. 5146-5159 ◽  
Author(s):  
Xuchu Wang ◽  
Minjing Shi ◽  
Dan Wang ◽  
Yueyi Chen ◽  
Fuge Cai ◽  
...  
Keyword(s):  
Hevea Brasiliensis ◽  
Rubber Particle ◽  
Comparative Proteomics ◽  
Particle Aggregation

A role for a Hevea latex lectin-like protein in mediating rubber particle aggregation and latex coagulation

2008 ◽  
Vol 69 (2) ◽  
pp. 339-347 ◽  
Author(s):  
Rapepun Wititsuwannakul ◽  
Piyaporn Pasitkul ◽  
Kamonwan Kanokwiroon ◽  
Dhirayos Wititsuwannakul
Keyword(s):  
Rubber Particle ◽  
Particle Aggregation ◽  
Latex Coagulation

scholarly journals Subcellular localization and interactions among rubber particle proteins from Hevea brasiliensis

2017 ◽  
Vol 68 (18) ◽  
pp. 5045-5055 ◽  
Author(s):  
Daniel Brown ◽  
Mistianne Feeney ◽  
Mathin Ahmadi ◽  
Chiara Lonoce ◽  
Roslinda Sajari ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Hevea Brasiliensis ◽  
Rubber Particle

scholarly journals PRODUKSI TANAMAN KARET (Hevea brasiliensis) PADA WAKTU PENGUMPULAN LATEKS YANG BERBEDA

2019 ◽  
Vol 3 (1) ◽  
pp. 27-34
Author(s):  
Mochlisin Andriyanto ◽  
Andi Wijaya ◽  
Junaidi . ◽  
Arief Rachmawan
Keyword(s):  
Flow Rate ◽  
Hevea Brasiliensis ◽  
Block Design ◽  
Rubber Particle ◽  
Rubber Tree ◽  
Total Solid ◽  
Solid Content ◽  
Total Solid Content ◽  
Randomized Block Design ◽  
Collection Period

Latex is a colloidal suspended of rubber particle in rubber tree (Hevea brasiliensis). Technically, the capacity of rubber productivity can be determined by latex collectionperiod after being tapped. The objective of research was to obtain the differences of latex collection period. The study was conducted in September-November 2017 at the Sungei Putih Research Center in Experimental Estate with PB 260 (7 years tapped) on BO-2 (S/2 d3.ET2.5% Ga1.0 6/y(m)).The experimental designused a Randomized Block Design (RBD) with four treatments of latex collection periodi.e 20, 40, 60, 80 minutes after being tapped with three replicates respectively. The parameters observed was gram/tree/tapping (g/p/s), kg/ha/years, latex flow rate (ml/minutes) and total solid content (%).The results showed that latex collection period was significantly different to g/p/s, kg/ha/years and latex flow rate. Total solid content (TSC) was not significantlydifferent in all treatments. The latex collection period of 20 minutes after being tapped had higher productivity than 40, 60, 80 minutes.

scholarly journals Structural and Functional Annotation of Transposable Elements Revealed a Potential Regulation of Genes Involved in Rubber Biosynthesis by TE-Derived siRNA Interference in Hevea brasiliensis

2020 ◽  
Vol 21 (12) ◽  
pp. 4220
Author(s):  
Shuangyang Wu ◽  
Romain Guyot ◽  
Stéphanie Bocs ◽  
Gaëtan Droc ◽  
Fetrina Oktavia ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transposable Elements ◽  
Hevea Brasiliensis ◽  
Genomic Sequence ◽  
Rubber Particle ◽  
Small Interfering Rnas ◽  
Rubber Biosynthesis ◽  
Major Function ◽  
Transcription Start Sites ◽  
Genomic Environment

The natural rubber biosynthetic pathway is well described in Hevea, although the final stages of rubber elongation are still poorly understood. Small Rubber Particle Proteins and Rubber Elongation Factors (SRPPs and REFs) are proteins with major function in rubber particle formation and stabilization. Their corresponding genes are clustered on a scaffold1222 of the reference genomic sequence of the Hevea brasiliensis genome. Apart from gene expression by transcriptomic analyses, to date, no deep analyses have been carried out for the genomic environment of SRPPs and REFs loci. By integrative analyses on transposable element annotation, small RNAs production and gene expression, we analysed their role in the control of the transcription of rubber biosynthetic genes. The first in-depth annotation of TEs (Transposable Elements) and their capacity to produce TE-derived siRNAs (small interfering RNAs) is presented, only possible in the Hevea brasiliensis clone PB 260 for which all data are available. We observed that 11% of genes are located near TEs and their presence may interfere in their transcription at both genetic and epigenetic level. We hypothesized that the genomic environment of rubber biosynthesis genes has been shaped by TE and TE-derived siRNAs with possible transcriptional interference on their gene expression. We discussed possible functionalization of TEs as enhancers and as donors of alternative transcription start sites in promoter sequences, possibly through the modelling of genetic and epigenetic landscapes.

In-depth proteome analysis of the rubber particle of Hevea brasiliensis (para rubber tree)

2013 ◽  
Vol 82 (1-2) ◽  
pp. 155-168 ◽  
Author(s):  
Longjun Dai ◽  
Guijuan Kang ◽  
Yu Li ◽  
Zhiyi Nie ◽  
Cuifang Duan ◽  
...  
Keyword(s):  
Hevea Brasiliensis ◽  
Rubber Particle ◽  
Rubber Tree ◽  
Proteome Analysis

Current Study on Structural Characterization and Unique Film Formation of Hevea brasiliensis Natural Rubber Latex

2013 ◽  
Vol 844 ◽  
pp. 498-501 ◽  
Author(s):  
Jitladda Sakdapipanich
Keyword(s):  
Natural Rubber ◽  
Hevea Brasiliensis ◽  
Film Formation ◽  
Rubber Particle ◽  
Particle Surface ◽  
Natural Rubber Latex ◽  
Force Microscopy ◽  
Latex Glove ◽  
Commercial Source

Hevea brasiliensis natural rubber (NR) latex is a very important commercial source of elastomers. It is not only a source for dry NR rubber but also the feedstock for the latex glove dipping industry. The particles of freshly tapped latex are known to be stabilized by proteins and lipids that come with the latex from the tree. These non-rubbers [, especially proteins and lipids, confer to the rubber and latex excellent properties unsurpassed by any synthetic latex [. Recent structural studies of NR revealed that the NR molecules comprise of 2 trans-isoprene units connected to a long-chain cis-isoprene units. Two terminal groups, referred to as ω and α, have been postulated to link with mono-and di-phosphate groups associated with phospholipids by H-bonding at the α-terminal, whereas the ω-terminal is a dimethylallyl group links to protein by H-bonding [. Although numerous studies have been published on the surface structure of the natural rubber particle [4-, none of these offered a direct in situ visualization of the intact particle surface. This is because of any attempt to remove this surface layer of proteins and lipids results in an immediate destabilization of the latex. The second part focused on the using of Atomic Force Microscopy (AFM) and Confocal Fluorescence microscopy to visualize and delineate the structure of the proteins and lipids layer on the latex particle surface in situ.

scholarly journals Rubber particle proteins REF1 and SRPP1 interact differently with native lipids extracted from Hevea brasiliensis latex

2017 ◽  
Vol 1859 (2) ◽  
pp. 201-210 ◽  
Author(s):  
Kanthida Wadeesirisak ◽  
Sabine Castano ◽  
Karine Berthelot ◽  
Laurent Vaysse ◽  
Frédéric Bonfils ◽  
...  
Keyword(s):  
Hevea Brasiliensis ◽  
Rubber Particle

scholarly journals Lipid Composition of Latex and Rubber Particles in Hevea brasiliensis and Taraxacum kok-saghyz

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5110
Author(s):  
Sung Woo Bae ◽  
Sunghee Jung ◽  
Sang Chul Choi ◽  
Mi Young Kim ◽  
Stephen Beungtae Ryu
Keyword(s):  
Natural Rubber ◽  
Lipid Composition ◽  
Hevea Brasiliensis ◽  
Particle Production ◽  
Rubber Particle ◽  
Rubber Tree ◽  
Rubber Particles ◽  
The Future

Natural rubber is usually synthesized in the rubber particles present in the latex of rubber-producing plants such as the Pará rubber tree (Hevea brasiliensis) and rubber dandelion (Taraxacum kok-saghyz). Since the detailed lipid compositions of fresh latex and rubber particles of the plants are poorly known, the present study reports detailed compound lipid composition, focusing on phospholipids and galactolipids in the latex and rubber particles of the plants. In the fresh latex and rubber particles of both plants, phospholipids were much more dominant (85–99%) compared to galactolipids. Among the nine classes of phospholipids, phosphatidylcholines (PCs) were most abundant, at ~80%, in both plants. Among PCs, PC (36:4) and PC (34:2) were most abundant in the rubber tree and rubber dandelion, respectively. Two classes of galactolipids, monogalactosyl diacylglycerol and digalactosyl diacylglycerol, were detected as 12% and 1%, respectively, of total compound lipids in rubber tree, whereas their percentages in the rubber dandelion were negligible (< 1%). Overall, the compound lipid composition differed only slightly between the fresh latex and the rubber particles of both rubber plants. These results provide fundamental data on the lipid composition of rubber particles in two rubber-producing plants, which can serve as a basis for artificial rubber particle production in the future.

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