N-Linked Glycan Structures of the Human Fcγ Receptors Produced in NS0 Cells

2013 ◽  
Vol 12 (8) ◽  
pp. 3721-3737 ◽  
Author(s):  
Eoin F. J. Cosgrave ◽  
Weston B. Struwe ◽  
Jerrard M. Hayes ◽  
David J. Harvey ◽  
Mark R. Wormald ◽  
...  
Keyword(s):  
Fcγ Receptors ◽  
Ns0 Cells

scholarly journals Mouse IgG3 binding to macrophage-like cells is prevented by deglycosylation of the antibody or by Accutase treatment of the cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alicja Karabasz ◽  
Monika Bzowska ◽  
Joanna Bereta ◽  
Maria Czarnek ◽  
Maja Sochalska ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Complex Network ◽  
Immune Cells ◽  
Specific Receptor ◽  
Fcγ Receptors ◽  
Specific Receptors ◽  
First Time

AbstractThe binding of mouse IgG3 to Fcγ receptors (FcγR) and the existence of a mouse IgG3-specific receptor have been discussed for 40 years. Recently, integrin beta-1 (ITGB1) was proposed to be a part of an IgG3 receptor involved in the phagocytosis of IgG3-coated pathogens. We investigated the interaction of mouse IgG3 with macrophage-like J774A.1 and P388D1 cells. The existence of an IgG3-specific receptor was verified using flow cytometry and a rosetting assay, in which erythrocytes clustered around the macrophage-like cells coated with an erythrocyte-specific IgG3. Our findings confirmed that receptors binding antigen-free IgG3 are present on J774A.1 and P388D1 cells. We demonstrated for the first time that the removal of N-glycans from IgG3 completely abolished its binding to the cells. Moreover, we discovered that the cells treated with Accutase did not bind IgG3, indicating that IgG3-specific receptors are substrates of this enzyme. The results of antibody-mediated blocking of putative IgG3 receptors suggested that apart from previously proposed ITGB1, FcγRII, FcγRIII, also additional, still unknown, receptor is involved in IgG3 binding. These findings indicate that there is a complex network of glycan-dependent interactions between mouse IgG3 and the surface of effector immune cells.

Immunocytochemical detection of Fcγ receptors in human atherosclerotic lesions

2001 ◽  
Vol 77 (3) ◽  
pp. 169-174 ◽  
Author(s):  
Nora R Ratcliffe ◽  
Susan M Kennedy ◽  
Peter M ◽  
Morganelli
Keyword(s):  
Fcγ Receptors ◽  
Atherosclerotic Lesions ◽  
Immunocytochemical Detection

Solubilization of human peripheral nerve Fcγ receptors and purification of a functional 40 kDa receptor

1989 ◽  
Vol 22 (4) ◽  
pp. 281-285 ◽  
Author(s):  
C.A. Vedeler ◽  
R. Matre ◽  
E.K. Kristoffersen
Keyword(s):  
Peripheral Nerve ◽  
Fcγ Receptors

scholarly journals Targeting to Fcγ Receptors, But Not CR3 (CD11b/CD18), Increases Clearance ofBordetella pertussis

2001 ◽  
Vol 183 (6) ◽  
pp. 871-879 ◽  
Author(s):  
Sandra M. M. Hellwig ◽  
Hans F. L. M. van Oirschot ◽  
Wouter L. W. Hazenbos ◽  
Annemiek B. van Spriel ◽  
Frits R. Mooi ◽  
...  
Keyword(s):  
Fcγ Receptors ◽  
Ofbordetella Pertussis

scholarly journals The yin and yang of Fcγ receptors in ITP

Blood ◽  
2008 ◽  
Vol 111 (3) ◽  
pp. 971-972 ◽  
Author(s):  
Steven E. McKenzie
Keyword(s):  
Fcγ Receptors ◽  
Yin And Yang

Modulation of Fcγ receptors on the human macrophage cell line U-937

1989 ◽  
Vol 120 (1) ◽  
pp. 174-187 ◽  
Author(s):  
Milton D. Rossman ◽  
Edgar Chen ◽  
Paul Chien ◽  
Alan D. Schreiber
Keyword(s):  
Cell Line ◽  
Human Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Fcγ Receptors

Active and passive re-expression of Fcγ receptors on human lymphocytes

1980 ◽  
Vol 10 (5) ◽  
pp. 352-358 ◽  
Author(s):  
Christiane Samarut ◽  
Jean-Pierre Revillard
Keyword(s):  
Human Lymphocytes ◽  
Fcγ Receptors
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