Dimethyl-Labeling-Based Protein Quantification and Pathway Search: A Novel Method of Spinal Cord Analysis Applicable for Neurological Studies

2013 ◽  
Vol 12 (5) ◽  
pp. 2245-2252 ◽  
Author(s):  
Ping Sui ◽  
Hiroyuki Watanabe ◽  
Michael H. Ossipov ◽  
Frank Porreca ◽  
Georgy Bakalkin ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Protein Quantification ◽  
Dimethyl Labeling ◽  
Novel Method ◽  
Pathway Search

scholarly journals A Novel Method for Simultaneous Anterograde and Retrograde Labeling of Spinal Cord Motor Tracts in the Same Animal

2001 ◽  
Vol 49 (9) ◽  
pp. 1111-1122 ◽  
Author(s):  
Eve C. Tsai ◽  
Rita L. van Bendegem ◽  
Steven W. Hwang ◽  
Charles H. Tator
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Regeneration ◽  
Adult Rats ◽  
Retrograde Labeling ◽  
Repair Strategy ◽  
Motor Tracts ◽  
Novel Method ◽  
Adult Spinal Cord ◽  
Anterograde And Retrograde Tracing ◽  
Spinal Cord Repair

Examination of repaired spinal cord tracts has usually required separate groups of animals for anterograde and retrograde tracing owing to the incompatibility of techniques such as tissue fixation. However, anterograde and retrograde labeling of different animals subjected to the same repair may not allow accurate examination of that repair strategy because widely variable results can occur in animals subjected to the same strategy. We have developed a reliable method of labeling spinal cord motor tracts bidirectionally in the same animal using DiI, a lipophilic dye, to anterogradely label the corticospinal tract and Fluoro-Gold (FG) to retrogradely label cortical and brainstem neurons of several spinal cord motor tracts in normal and injured adult rats. Other tracer combinations (lipophilic dyes or fluorescent dextrans) were also investigated but were less effective. We also developed methods to minimize autofluorescence with the DiI/FG technique, and found that the DiI/FG technique is compatible with decalcification and immunohistochemistry for several markers relevant for studies of spinal cord regeneration. Thus, the use of anterograde DiI and retrograde FG is a novel technique for bidirectional labeling of the motor tracts of the adult spinal cord with fluorescent tracers and should be useful for demonstrating neurite regeneration in studies of spinal cord repair. (J Histochem Cytochem 49:1111–1122, 2001)

Sa1560 - A Novel Method of Spinal Cord Stimulation for Treating Gastroparesis a Priliminary Study in Dogs

2018 ◽  
Vol 154 (6) ◽  
pp. S-311 ◽  
Author(s):  
Lei Tu ◽  
Bo Zhang ◽  
Feng Ji ◽  
YI Yang ◽  
Payam Gharibani ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Stimulation ◽  
Novel Method

A novel method of organotypic spinal cord slice culture in rats

Neuroreport ◽  
2017 ◽  
Vol 28 (16) ◽  
pp. 1097-1102 ◽  
Author(s):  
Jing-Jie Liu ◽  
Ya-Juan Huang ◽  
Li Xiang ◽  
Feng Zhao ◽  
Sheng-Li Huang
Keyword(s):  
Spinal Cord ◽  
Slice Culture ◽  
Spinal Cord Slice ◽  
Novel Method

scholarly journals Novel Concept of Motor Functional Analysis for Spinal Cord Injury in Adult Mice

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Munehisa Shinozaki ◽  
Yuichiro Takahashi ◽  
Masahiko Mukaino ◽  
Nobuhito Saito ◽  
Yoshiaki Toyama ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Open Field ◽  
Injury Severity ◽  
Basic Research ◽  
Maximum Speed ◽  
Adult Mice ◽  
Cord Injury ◽  
Novel Method ◽  
Novel Concept

In basic research on spinal cord injury (SCI), behavioral evaluation of the SCI animal model is critical. However, it is difficult to accurately evaluate function in the mouse SCI model due to the small size of mice. Although the open-field scoring scale is an outstanding appraisal method, supplementary objective tests are required. Using a compact SCANET system, in which a mouse carries out free movement for 5 min, we developed a novel method to detect locomotor ability. A SCANET system samples the horizontal coordinates of a mouse every 0.1 s, and both the speed and acceleration of its motion are calculated at each moment. It was found that the maximum speed and acceleration of motion over 5 min varied by injury severity. Moreover, these values were significantly correlated with open-field scores. The maximum speed and acceleration of SCI model mice using a SCANET system are objective, easy to obtain, and reproducible for evaluating locomotive function.

scholarly journals Novel Method for Analyzing Locomotor Ability After Spinal Cord Injury in Rats: Technical Note

2013 ◽  
Vol 53 (12) ◽  
pp. 907-913 ◽  
Author(s):  
Munehisa SHINOZAKI ◽  
Akimasa YASUDA ◽  
Satoshi NORI ◽  
Nobuhito SAITO ◽  
Yoshiaki TOYAMA ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Technical Note ◽  
Cord Injury ◽  
Novel Method ◽  
Locomotor Ability
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