A Strategy for Large-Scale Phosphoproteomics and SRM-Based Validation of Human Breast Cancer Tissue Samples

2012 ◽  
Vol 11 (11) ◽  
pp. 5311-5322 ◽  
Author(s):  
Ryohei Narumi ◽  
Tatsuo Murakami ◽  
Takahisa Kuga ◽  
Jun Adachi ◽  
Takashi Shiromizu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Large Scale ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Human Breast ◽  
Human Breast Cancer Tissue ◽  
Tissue Samples

Abstract 3309: Identifying glycoproteins in ER+ and ER- human breast cancer tissue samples

2010 ◽  
Author(s):  
Suzan Semaan ◽  
Xu Eddy Wang ◽  
R. Emmett ◽  
Alan G. Marshall ◽  
Qing-Xiang Amy Sang
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Human Breast ◽  
Human Breast Cancer Tissue ◽  
Tissue Samples

Detection of DNA fragmentation in human breast cancer tissue by an antibody specific to single-stranded DNA

Breast Cancer ◽  
1998 ◽  
Vol 5 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Shunzo Kobayashi ◽  
Hirotaka Iwase ◽  
Yoshihiko Kawarada ◽  
Naoyuki Miura ◽  
Toshihiro Sugiyama ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Fragmentation ◽  
Human Breast Cancer ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Human Breast ◽  
Human Breast Cancer Tissue ◽  
Single Stranded Dna

scholarly journals Development of an ELISA for Measurement of BCAR1 Protein in Human Breast Cancer Tissue

2004 ◽  
Vol 50 (8) ◽  
pp. 1356-1363 ◽  
Author(s):  
Nicolai Grebenchtchikov ◽  
Arend Brinkman ◽  
Simone P J van Broekhoven ◽  
Danielle de Jong ◽  
Anneke Geurts-Moespot ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Western Blot ◽  
Human Breast Cancer ◽  
High Sensitivity ◽  
Cross Reactivity ◽  
Breast Cancer Tissue ◽  
Primary Breast Tumor ◽  
Cancer Tissue ◽  
Human Breast ◽  
Human Breast Cancer Tissue

Abstract Background: High concentrations of breast cancer anti-estrogen resistance 1 (BCAR1) protein measured by Western blotting in primary breast tumor cytosols are associated with early disease progression and failure of tamoxifen therapy. The aim of the present study was to develop an ELISA to measure BCAR1 quantitatively in extracts of human breast cancer tissue. Methods: A recombinant fragment of BCAR1 (the human homolog of murine p130Cas) was produced in bacterial M15 cells, purified, and injected into chickens and rabbits. The generated antibodies were affinity-purified and used for the construction of an ELISA. After validation, the results obtained with the ELISA were compared with Western blot findings on primary breast tumors. Results: The detection limit the BCAR1 ELISA was 0.0031 μg/L, and the within-run imprecision (CV) was <20% at concentrations down to 0.004 μg/L. The within-run imprecision (CV) was 1.0–7.2%, and the between-run CV was 3.6–5.4%. There was no cross-reactivity with family member HEF1. The assay exhibited parallelism of results between serial dilutions and a mean recovery (range) of 96 (79–118)%. Conclusions: The ELISA measures BCAR1 in human breast cancer cytosols with high sensitivity and specificity. The assay can be used to confirm and to quantitatively extend previous semiquantitative Western blot data on the prognostic and predictive value of BCAR1 in human breast cancer; it can also be applied for other diseases.

Acid phosphatase in human breast cancer tissue

Cancer ◽  
1984 ◽  
Vol 53 (2) ◽  
pp. 301-305 ◽  
Author(s):  
Jorge E. Filmus ◽  
Osvaldo L. Podhajcer ◽  
Eduardo Mareso ◽  
Natalio Guman ◽  
José Mordoh
Keyword(s):  
Breast Cancer ◽  
Acid Phosphatase ◽  
Human Breast Cancer ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Human Breast ◽  
Human Breast Cancer Tissue
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