Identification of Potential Universal Vaccine Candidates against Group A Streptococcus by Using High Throughput in Silico and Proteomics Approach

2012 ◽  
Vol 12 (1) ◽  
pp. 336-346 ◽  
Author(s):  
Abhinay Sharma ◽  
Deepak Kumar Arya ◽  
Vivek Sagar ◽  
René Bergmann ◽  
Gursharan Singh Chhatwal ◽  
...  
Keyword(s):  
High Throughput ◽  
In Silico ◽  
Group A Streptococcus ◽  
Universal Vaccine ◽  
Vaccine Candidates ◽  
Proteomics Approach ◽  
Group A

scholarly journals Multi High-Throughput Approach for Highly Selective Identification of Vaccine Candidates: the Group A Streptococcus Case

2012 ◽  
Vol 11 (6) ◽  
pp. M111.015693 ◽  
Author(s):  
Giuliano Bensi ◽  
Marirosa Mora ◽  
Giovanna Tuscano ◽  
Massimiliano Biagini ◽  
Emiliano Chiarot ◽  
...  
Keyword(s):  
High Throughput ◽  
Group A Streptococcus ◽  
Vaccine Candidates ◽  
Group A

Comparative in silico analysis of two vaccine candidates for group A streptococcus predicts that they both may have similar safety profiles

Vaccine ◽  
2007 ◽  
Vol 25 (18) ◽  
pp. 3567-3573 ◽  
Author(s):  
Melkote S. Shaila ◽  
Rabindranath Nayak ◽  
Savitha S. Prakash ◽  
Melina Georgousakis ◽  
Evelyn Brandt ◽  
...  
Keyword(s):  
In Silico ◽  
Group A Streptococcus ◽  
In Silico Analysis ◽  
Vaccine Candidates ◽  
Group A ◽  
Silico Analysis

scholarly journals Cholic Acid-based Delivery System for Vaccine Candidates against Group A Streptococcus

2019 ◽  
Vol 10 (9) ◽  
pp. 1253-1259 ◽  
Author(s):  
Armira Azuar ◽  
Lili Zhao ◽  
Tsui Ting Hei ◽  
Reshma J. Nevagi ◽  
Stacey Bartlett ◽  
...  
Keyword(s):  
Cholic Acid ◽  
Delivery System ◽  
Group A Streptococcus ◽  
Vaccine Candidates ◽  
Group A

Lipid Peptide Core Nanoparticles as Multivalent Vaccine Candidates against Streptococcus pyogenes

2012 ◽  
Vol 65 (1) ◽  
pp. 35 ◽  
Author(s):  
Mariusz Skwarczynski ◽  
Bibi Hamideh Parhiz ◽  
Fatemeh Soltani ◽  
Saranya Srinivasan ◽  
Khairul A. Kamaruzaman ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Subunit Vaccine ◽  
Click Reaction ◽  
Group A Streptococcus ◽  
Vaccine Candidates ◽  
Wide Range ◽  
Group A ◽  
Core Peptide ◽  
Aqueous Conditions ◽  
Peptide Core

Traditional vaccine approaches for Group A streptococcus (GAS) infection are inadequate owing to the host’s production of cross-reactive antibodies that recognize not only the bacteria but also human tissue. To overcome this problem a peptide subunit-based vaccine was proposed, which would incorporate only minimal non-cross reactive epitopes. However, special delivery systems/adjuvants were required because short peptides are not immunogenic. In this study we have incorporated two epitopes from two different GAS proteins into a lipid core peptide (LCP) self-adjuvanting delivery system to achieve better protection against a wide range of GAS serotypes. Multivalent and monovalent constructs were synthesized with the help of an azide alkyne cycloaddition (click) reaction and their ability to self-assemble under aqueous conditions was examined. The compounds significantly differed in their ability to form small size nanoparticles, which are believed to be most appropriate for peptide-based subunit vaccine delivery. The LCP conjugates possessing two different epitopes, in contrast to monoepitopic constructs, formed small nanoparticles (5–15 nm) presumably owing to a suitable hydrophilic-hydrophobic balance of the molecules.

scholarly journals A Proteomics Signature of Mild Hypospadias: A Pilot Study

2020 ◽  
Vol 8 ◽  
Author(s):  
Coriness Piñeyro-Ruiz ◽  
Horacio Serrano ◽  
Inmaculada Jorge ◽  
Eric Miranda-Valentin ◽  
Marcos R. Pérez-Brayfield ◽  
...  
Keyword(s):  
High Throughput ◽  
Energy Production ◽  
Isotope Labeling ◽  
Cellular Processes ◽  
Proteomics Approach ◽  
Liquid Chromatography Tandem Mass ◽  
Congenital Condition ◽  
Group A ◽  
Control Samples

Background and Objective: Mild hypospadias is a birth congenital condition characterized by the relocation of the male urethral meatus from its typical anatomical position near the tip of the glans penis, to a lower ventral position up to the brim of the glans corona, which can also be accompanied by foreskin ventral deficiency. For the most part, a limited number of cases have known etiology. We have followed a high-throughput proteomics approach to study the proteome in mild hypospadias patients.Methods: Foreskin samples from patients with mild hypospadias were collected during urethroplasty, while control samples were collected during elective circumcision (n = 5/group). A high-throughput, quantitative proteomics approach based on multiplexed peptide stable isotope labeling (SIL) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was used to ascertain protein abundance changes in hypospadias patients when compared to control samples.Results: A total of 4,815 proteins were quantitated (2,522 with at least two unique peptides). One hundred and thirty-three proteins from patients with mild hypospadias showed significant abundance changes with respect to control samples, where 38 proteins were increased, and 95 proteins were decreased. Unbiased functional biological analysis revealed that both mitochondrial energy production and apoptotic signaling pathways were enriched in mild hypospadias.Conclusions: This first comprehensive proteomics characterization of mild hypospadias shows molecular changes associated with essential cellular processes related to energy production and apoptosis. Further evaluation of the proteome may expand the search of novel candidates in the etiology of mild hypospadias and could also lead to the identification of biomarkers for this congenital urogenital condition.

Group A streptococcus inhibitors by high-throughput virtual screening

2014 ◽  
Vol 82 ◽  
pp. 120-126
Author(s):  
Haipeng Hu ◽  
Shuli Mao ◽  
Julia V. Bugrysheva ◽  
Sarah Pruett ◽  
Dennis C. Liotta ◽  
...  
Keyword(s):  
Virtual Screening ◽  
High Throughput ◽  
Group A Streptococcus ◽  
Group A ◽  
High Throughput Virtual Screening

Structure–activity relationship of lipid core peptide-based Group A Streptococcus vaccine candidates

2016 ◽  
Vol 24 (14) ◽  
pp. 3095-3101 ◽  
Author(s):  
Amy Chan ◽  
Waleed M. Hussein ◽  
Khairunnisa Abdul Ghaffar ◽  
Nirmal Marasini ◽  
Ahmed Mostafa ◽  
...  
Keyword(s):  
Group A Streptococcus ◽  
Structure Activity Relationship ◽  
Activity Relationship ◽  
Vaccine Candidates ◽  
Lipid Core ◽  
Structure Activity ◽  
Group A ◽  
Core Peptide ◽  
Relationship Of

Structure–Activity Analysis of Cyclic Multicomponent Lipopeptide Self-Adjuvanting Vaccine Candidates Presenting Group A Streptococcus Antigens

2020 ◽  
Vol 63 (10) ◽  
pp. 5387-5397
Author(s):  
Harrison Y. R. Madge ◽  
Hansa Sharma ◽  
Waleed M. Hussein ◽  
Zeinab G. Khalil ◽  
Robert J. Capon ◽  
...  
Keyword(s):  
Group A Streptococcus ◽  
Activity Analysis ◽  
Vaccine Candidates ◽  
Structure Activity ◽  
Group A

Structure–activity relationship of lipopeptide Group A streptococcus (GAS) vaccine candidates on toll-like receptor 2

Vaccine ◽  
2010 ◽  
Vol 28 (10) ◽  
pp. 2243-2248 ◽  
Author(s):  
Mehfuz Zaman ◽  
Abu-Baker M. Abdel-Aal ◽  
Karen S.M. Phillipps ◽  
Yoshio Fujita ◽  
M.F. Good ◽  
...  
Keyword(s):  
Group A Streptococcus ◽  
Structure Activity Relationship ◽  
Activity Relationship ◽  
Toll Like Receptor ◽  
Vaccine Candidates ◽  
Structure Activity ◽  
Group A ◽  
Toll Like Receptor 2 ◽  
Relationship Of
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