Patterns of Gene and Metabolite Define the Effects of Extracellular Osmolality on Kidney Collecting Duct

2012 ◽  
Vol 11 (7) ◽  
pp. 3816-3828 ◽  
Author(s):  
Hyo-Jung Choi ◽  
Yu-Jeong Yoon ◽  
Yong-Kook Kwon ◽  
Yu-Jung Lee ◽  
Sehyun Chae ◽  
...  
Keyword(s):  
Collecting Duct ◽  
Kidney Collecting Duct

scholarly journals Expression of VAMP-2-like protein in kidney collecting duct intracellular vesicles. Colocalization with Aquaporin-2 water channels.

1995 ◽  
Vol 96 (4) ◽  
pp. 1834-1844 ◽  
Author(s):  
S Nielsen ◽  
D Marples ◽  
H Birn ◽  
M Mohtashami ◽  
N O Dalby ◽  
...  
Keyword(s):  
Collecting Duct ◽  
Water Channels ◽  
Kidney Collecting Duct ◽  
Aquaporin 2 ◽  
Intracellular Vesicles

Expression, functional analysis, and in situ hybridization of a cloned rat kidney collecting duct water channel

1994 ◽  
Vol 266 (1) ◽  
pp. C189-C197 ◽  
Author(s):  
T. Ma ◽  
H. Hasegawa ◽  
W. R. Skach ◽  
A. Frigeri ◽  
A. S. Verkman
Keyword(s):  
Base Pair ◽  
Collecting Duct ◽  
Rat Kidney ◽  
Water Channel ◽  
Cloning And Expression ◽  
Base Pairs ◽  
Kidney Medulla ◽  
Coding Sequence ◽  
Kidney Collecting Duct ◽  
Protein Size

The cloning and expression of an apical membrane water channel from rat kidney collecting duct (WCH-CD) homologous to a 28-kDa integral membrane protein (CHIP28) was reported recently (K. Fushimi, S. Uchida, Y. Hara, Y. Hirata, F. Marumo, and S. Sasaki. Nature Lond. 361: 549-552, 1993). We obtained an approximately 1.8-kilobase clone from a rat kidney lambda gt10 cDNA library by a polymerase chain reaction cloning method; whereas the coding sequence (814 base pairs, predicted protein size 29 kDa) was identical to that reported, we identified an in-frame ATG codon at base pair -123 predicting a protein size of 33 kDa. On Northern blots probed by cDNAs corresponding to the WCH-CD coding sequence (base pairs +1 to +814) or 5'-untranslated sequence (-403 to -16), a single band at 1.9 kilobases was observed in kidney medulla greater than in cortex but not in other tissues; mRNA expression was increased strongly by dehydration. Translation and oocyte expression studies were performed to identify the translation start site. The short (base pairs +1 to +814) and long (base pairs -123 to +814) cDNAs were subcloned in vector pSP64 containing the 5'-untranslated Xenopus globin sequence upstream to the ATGs; a 30-base pair c-myc sequence was engineered at the COOH- terminal for antibody recognition.(ABSTRACT TRUNCATED AT 250 WORDS)

Generation of AQP2-Cre transgenic mini-pigs specifically expressing Cre recombinase in kidney collecting duct cells

2013 ◽  
Vol 23 (2) ◽  
pp. 365-375 ◽  
Author(s):  
Weiwei Luo ◽  
Zhanjun Li ◽  
Yongye Huang ◽  
Yang Han ◽  
Chaogang Yao ◽  
...  
Keyword(s):  
Collecting Duct ◽  
Cre Recombinase ◽  
Kidney Collecting Duct ◽  
Duct Cells ◽  
Collecting Duct Cells ◽  
Mini Pigs

MAP kinases‐Regulated Proteins in Downstream Signaling Pathways of Vasopressin V2 Receptor in Kidney Collecting Duct

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Hyo-Ju Jang ◽  
Hyun jun Jung ◽  
Hyo-Jung Choi ◽  
Eui-Jung Park ◽  
Hye-Jeong Park ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Map Kinases ◽  
Collecting Duct ◽  
Downstream Signaling ◽  
Kidney Collecting Duct ◽  
Vasopressin V2 Receptor ◽  
V2 Receptor

Cell volume regulation of rat kidney collecting duct epithelial cells in hypotonic medium

2011 ◽  
Vol 436 (1) ◽  
pp. 13-15 ◽  
Author(s):  
E. I. Solenov ◽  
G. S. Baturina ◽  
A. V. Ilyaskin ◽  
L. Ye. Katkova ◽  
L. N. Ivanova
Keyword(s):  
Epithelial Cells ◽  
Cell Volume ◽  
Volume Regulation ◽  
Collecting Duct ◽  
Rat Kidney ◽  
Cell Volume Regulation ◽  
Hypotonic Medium ◽  
Kidney Collecting Duct

scholarly journals Apical endosomes isolated from kidney collecting duct principal cells lack subunits of the proton pumping ATPase.

1992 ◽  
Vol 119 (1) ◽  
pp. 111-122 ◽  
Author(s):  
I Sabolic ◽  
F Wuarin ◽  
L B Shi ◽  
A S Verkman ◽  
D A Ausiello ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Proton Pump ◽  
Transmembrane Domain ◽  
Collecting Duct ◽  
Water Channels ◽  
Proton Pumping ◽  
Apical Plasma Membrane ◽  
Principal Cells ◽  
Kidney Collecting Duct ◽  
Early Endosomes

Endocytic vesicles that are involved in the vasopressin-stimulated recycling of water channels to and from the apical membrane of kidney collecting duct principal cells were isolated from rat renal papilla by differential and Percoll density gradient centrifugation. Fluorescence quenching measurements showed that the isolated vesicles maintained a high, HgCl2-sensitive water permeability, consistent with the presence of vasopressin-sensitive water channels. They did not, however, exhibit ATP-dependent luminal acidification, nor any N-ethylmaleimide-sensitive ATPase activity, properties that are characteristic of most acidic endosomal compartments. Western blotting with specific antibodies showed that the 31- and 70-kD cytoplasmically oriented subunits of the vacuolar proton pump were not detectable in these apical endosomes from the papilla, whereas they were present in endosomes prepared in parallel from the cortex. In contrast, the 56-kD subunit of the proton pump was abundant in papillary endosomes, and was localized at the apical pole of principal cells by immunocytochemistry. Finally, an antibody that recognizes the 16-kD transmembrane subunit of oat tonoplast ATPase cross-reacted with a distinct 16-kD band in cortical endosomes, but no 16-kD band was detectable in endosomes from the papilla. This antibody also recognized a 16-kD band in affinity-purified H+ ATPase preparations from bovine kidney medulla. Therefore, early endosomes derived from the apical plasma membrane of collecting duct principal cells fail to acidify because they lack functionally important subunits of a vacuolar-type proton pumping ATPase, including the 16-kD transmembrane domain that serves as the proton-conducting channel, and the 70-kD cytoplasmic subunit that contains the ATPase catalytic site. This specialized, non-acidic early endosomal compartment appears to be involved primarily in the hormonally induced recycling of water channels to and from the apical plasma membrane of vasopressin-sensitive cells in the kidney collecting duct.

Study of the reaction of kidney collecting duct principal cells to hypotonic shock. Experiment and mathematical model

BIOPHYSICS ◽  
2011 ◽  
Vol 56 (3) ◽  
pp. 516-524 ◽  
Author(s):  
A. V. Ilyaskin ◽  
G. S. Baturina ◽  
D. A. Medvedev ◽  
A. P. Ershov ◽  
E. I. Solenov
Keyword(s):  
Mathematical Model ◽  
Collecting Duct ◽  
Hypotonic Shock ◽  
Principal Cells ◽  
Kidney Collecting Duct ◽  
Shock Experiment
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