Mass Spectrometrical Identification of Hippocampal NMDA Receptor Subunits NR1, NR2A–D and Five Novel Phosphorylation Sites on NR2A and NR2B

2012 ◽  
Vol 11 (3) ◽  
pp. 1891-1896 ◽  
Author(s):  
Maryam Ghafari ◽  
Harald Höger ◽  
Soheil Keihan Falsafi ◽  
Nina Russo-Schlaff ◽  
Arnold Pollak ◽  
...  
Keyword(s):  
Nmda Receptor ◽  
Phosphorylation Sites ◽  
Nmda Receptor Subunits

scholarly journals Developmental up-regulation of NMDA receptors in the prefrontal cortex and hippocampus of mGlu5 receptor knock-out mice

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Tiziana Imbriglio ◽  
Remy Verhaeghe ◽  
Nico Antenucci ◽  
Stefania Maccari ◽  
Giuseppe Battaglia ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Nmda Receptor ◽  
Metabotropic Glutamate Receptors ◽  
Adult Life ◽  
Developmental Time ◽  
Postnatal Life ◽  
Developmental Studies ◽  
Knock Out ◽  
Nmda Receptor Subunits ◽  
The Impact

AbstractmGlu5 metabotropic glutamate receptors are highly expressed and functional in the early postnatal life, and are known to positively modulate NMDA receptor function. Here, we examined the expression of NMDA receptor subunits and interneuron-related genes in the prefrontal cortex and hippocampus of mGlu5−/− mice and wild-type littermates at three developmental time points (PND9, − 21, and − 75). We were surprised to find that expression of all NMDA receptor subunits was greatly enhanced in mGlu5−/− mice at PND21. In contrast, at PND9, expression of the GluN2B subunit was enhanced, whereas expression of GluN2A and GluN2D subunits was reduced in both regions. These modifications were transient and disappeared in the adult life (PND75). Changes in the transcripts of interneuron-related genes (encoding parvalbumin, somatostatin, vasoactive intestinal peptide, reelin, and the two isoforms of glutamate decarboxylase) were also observed in mGlu5−/− mice across postnatal development. For example, the transcript encoding parvalbumin was up-regulated in the prefrontal cortex of mGlu5−/− mice at PND9 and PND21, whereas it was significantly reduced at PND75. These findings suggest that in mGlu5−/− mice a transient overexpression of NMDA receptor subunits may compensate for the lack of the NMDA receptor partner, mGlu5. Interestingly, in mGlu5−/− mice the behavioral response to the NMDA channel blocker, MK-801, was significantly increased at PND21, and largely reduced at PND75. The impact of adaptive changes in the expression of NMDA receptor subunits should be taken into account when mGlu5−/− mice are used for developmental studies.

scholarly journals Biochemical and electrophysiological characterization of N- glycans on NMDA receptor subunits

2016 ◽  
Vol 138 (4) ◽  
pp. 546-556 ◽  
Author(s):  
Martina Kaniakova ◽  
Katarina Lichnerova ◽  
Kristyna Skrenkova ◽  
Ladislav Vyklicky ◽  
Martin Horak
Keyword(s):  
Nmda Receptor ◽  
Nmda Receptor Subunits ◽  
Electrophysiological Characterization

103 Distinct laminar distribution of NMDA receptor subunits in the mouse hippocampus

1997 ◽  
Vol 28 ◽  
pp. S19
Author(s):  
Masahiko Watanabe ◽  
Kenji Sakimura ◽  
Masayoshi Mishina ◽  
Yoshiro Inoue
Keyword(s):  
Nmda Receptor ◽  
Nmda Receptor Subunits ◽  
Laminar Distribution ◽  
Mouse Hippocampus

Synaptic localization of NMDA receptor subunits in the rat retina

2000 ◽  
Vol 420 (1) ◽  
pp. 98-112 ◽  
Author(s):  
Erica L. Fletcher ◽  
Iris Hack ◽  
Johann Helmut Brandst�tter ◽  
Heinz W�ssle
Keyword(s):  
Nmda Receptor ◽  
Synaptic Localization ◽  
Rat Retina ◽  
Nmda Receptor Subunits

scholarly journals Acute and Chronic Sleep Deprivation-Related Changes in N-methyl-D-aspartate Receptor—Nitric Oxide Signalling in the Rat Cerebral Cortex with Reference to Aging and Brain Lateralization

2019 ◽  
Vol 20 (13) ◽  
pp. 3273 ◽  
Author(s):  
Zdenka Kristofikova ◽  
Jana Sirova ◽  
Jan Klaschka ◽  
Saak V. Ovsepian
Keyword(s):  
Nitric Oxide ◽  
Nmda Receptor ◽  
Sleep Deprivation ◽  
Nmda Receptor Subunits ◽  
Aspartate Receptor ◽  
Age Dependent ◽  
Chronic Sleep Deprivation ◽  
Nos Isoforms ◽  
The Impact ◽  
Chronic Sleep

Aging and chronic sleep deprivation (SD) are well-recognized risk factors for Alzheimer’s disease (AD), with N-methyl-D-aspartate receptor (NMDA) and downstream nitric oxide (NO) signalling implicated in the process. Herein, we investigate the impact of the age- and acute or chronic SD-dependent changes on the expression of NMDA receptor subunits (NR1, NR2A, and NR2B) and on the activities of NO synthase (NOS) isoforms in the cortex of Wistar rats, with reference to cerebral lateralization. In young adult controls, somewhat lateralized seasonal variations in neuronal and endothelial NOS have been observed. In aged rats, overall decreases in NR1, NR2A, and NR2B expression and reduction in neuronal and endothelial NOS activities were found. The age-dependent changes in NR1 and NR2B significantly correlated with neuronal NOS in both hemispheres. Changes evoked by chronic SD (dysfunction of endothelial NOS and the increasing role of NR2A) differed from those evoked by acute SD (increase in inducible NOS in the right side). Collectively, these results demonstrate age-dependent regulation of the level of NMDA receptor subunits and downstream NOS isoforms throughout the rat brain, which could be partly mimicked by SD. As described herein, age and SD alterations in the prevalence of NMDA receptors and NOS could contribute towards cognitive decline in the elderly, as well as in the pathobiology of AD and the neurodegenerative process.

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