Comparative Proteomic Analysis of Proteins in Response to Simulated Acid Rain inArabidopsis

2011 ◽  
Vol 10 (5) ◽  
pp. 2579-2589 ◽  
Author(s):  
Ting-Wu Liu ◽  
Bin Fu ◽  
Li Niu ◽  
Juan Chen ◽  
Wen-Hua Wang ◽  
...  
Keyword(s):  
Acid Rain ◽  
Proteomic Analysis ◽  
Simulated Acid Rain ◽  
Comparative Proteomic Analysis

Effect of Simulated Acid Rain on Gas Exchanges of Three Compositae Invasive Plants

2013 ◽  
Vol 48 (2) ◽  
pp. 160-167
Author(s):  
Song Liying ◽  
Ke Zhanhong ◽  
Sun Lanlan ◽  
Peng Changlian
Keyword(s):  
Acid Rain ◽  
Invasive Plants ◽  
Simulated Acid Rain ◽  
Gas Exchanges

scholarly journals Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

2021 ◽  
Vol 22 (12) ◽  
pp. 6323
Author(s):  
Alexander L. Rusanov ◽  
Peter M. Kozhin ◽  
Olga V. Tikhonova ◽  
Victor G. Zgoda ◽  
Dmitry S. Loginov ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Living Organism ◽  
Peritoneal Macrophages ◽  
In Vitro Models ◽  
Individual Characteristics ◽  
Essential Proteins ◽  
Comparative Proteomic Analysis ◽  
Immortalized Cell ◽  
The Individual

In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.

Sign in / Sign up

Export Citation Format

Share Document