Reproducibility of Protein Identification of Selected Cell Types in Barrett’s Esophagus Analyzed by Combining Laser-Capture Microdissection and Mass Spectrometry

2011 ◽  
Vol 10 (1) ◽  
pp. 288-298 ◽  
Author(s):  
Christoph Stingl ◽  
Frederike G. I. van Vilsteren ◽  
Coskun Guzel ◽  
Fiebo J. W. ten Kate ◽  
Mike Visser ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Barrett’S Esophagus ◽  
Laser Capture Microdissection ◽  
Barrett's Esophagus ◽  
Protein Identification ◽  
Cell Types ◽  
Laser Capture

scholarly journals Immunoreactivity of cytokeratins 7 and 20 in goblet cells and columnar blue cells in patients with endoscopic evidence of Barrett's esophagus

2009 ◽  
Vol 46 (2) ◽  
pp. 127-131 ◽  
Author(s):  
João Carlos Cantarelli Jr. ◽  
Renato Borges Fagundes ◽  
Luise Meurer ◽  
Marta Pires da Rocha ◽  
André Nicola ◽  
...  
Keyword(s):  
Intestinal Metaplasia ◽  
Barrett’S Esophagus ◽  
Alcian Blue ◽  
Barrett's Esophagus ◽  
Immunohistochemical Staining ◽  
Esophagogastric Junction ◽  
Cell Types ◽  
Goblet Cells ◽  
Cytokeratin 20 ◽  
Cytokeratin 7

CONTEXT: Barrett's esophagus is characterized by the presence of goblet cells. However, when alcian-blue is utilized, another type of cells, called columnar blue cells, is frequently present in the distal esophagus of patients with endoscopic evidence of Barrett's esophagus. Cytokeratin 7 and 20 immunoreactivity has been previously studied in areas of intestinal metaplasia at the esophagogastric junction. However, the expression of these cytokeratins in columnar blue cells has not been characterized. OBJECTIVE: To compare the expression of cytokeratin 7 and 20 in goblet cells and columnar blue cells in patients with endoscopic evidence of Barrett's esophagus. METHODS: Biopsies from 86 patients with endoscopic evidence of Barrett's esophagus were evaluated. The biopsies were stained for cytokeratin 7 and 20. RESULTS: Goblet cells were present in 75 cases and columnar blue cells in 50 cases. Overall, cytokeratin 7 expression was similar in goblet cells and columnar blue cells (P = 0.25), while cytokeratin 20 was more common in goblet cells (P <0.001). In individuals with both cell types, however, cytokeratin 7 staining was the same in goblet and columnar blue cells in 95% of the cases, and cytokeratin 20 staining was the same in 77%. CONCLUSION: Goblet cells and columnar blue cells have similar immunohistochemical staining patterns for cytokeratins 7 and 20 in patients with endoscopic evidence of Barrett's esophagus.

Proteome-wide analysis of head and neck squamous cell carcinomas using laser-capture microdissection and tandem mass spectrometry

Oral Oncology ◽  
2005 ◽  
Vol 41 (2) ◽  
pp. 183-199 ◽  
Author(s):  
Haven Baker ◽  
Vyomesh Patel ◽  
Alfredo A. Molinolo ◽  
Edward J. Shillitoe ◽  
John F. Ensley ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Head And Neck ◽  
Squamous Cell ◽  
Laser Capture Microdissection ◽  
Squamous Cell Carcinomas ◽  
Tandem Mass ◽  
Laser Capture
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