scholarly journals Research Profile: The cervical–vaginal fluid proteome and possible biomarkers of preterm birth

2007 ◽  
Vol 6 (4) ◽  
pp. 1241-1242 ◽  
Author(s):  
Katie Cottingham
Keyword(s):  
Preterm Birth ◽  
Vaginal Fluid ◽  
Research Profile

scholarly journals Vaginal Microbiota, Genital Inflammation and Extracellular Matrix Remodelling Collagenase: MMP-9 in Pregnant Women With HIV, a Potential Preterm Birth Mechanism Warranting Further Exploration

2021 ◽  
Vol 11 ◽  
Author(s):  
Charlotte-Eve S. Short ◽  
Rachael A. Quinlan ◽  
Xuan Wang ◽  
Veronica Georgiana Preda ◽  
Ann Smith ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Pregnant Women ◽  
Genital Tract ◽  
Amplicon Sequencing ◽  
Vaginal Microbiota ◽  
Vaginal Fluid ◽  
Polymorphonuclear Leucocytes ◽  
Genital Tract Infection ◽  
Maternal Characteristics ◽  
Tnf Α

BackgroundPregnant women living with HIV infection (PWLWH) have elevated rates of preterm birth (PTB) in which HIV and cART are implicated. PWLWH also have a high prevalence of adverse vaginal microbiota, which associate with genital tract inflammation. The mechanism underlying PTB in PWLWH is unknown. We present the first data in PWLWH on genital-tract matrix-metalloproteinase-9(MMP-9), an important collagenase implicated in labour onset, and tissue inhibitor of metalloproteinases-1(TIMP-1) and explore correlations with local inflammation and vaginal bacteria.Material and MethodsCervical vaginal fluid (CVF) collected by a soft cup and high vaginal swabs (HVS) were obtained from PWLWH and HIV uninfected pregnant women (HUPW) at three antenatal time points. Maternal characteristics, combination antiretroviral therapy (cART) exposure, and pregnancy outcome were recorded. Concentrations of MMP-9, TIMP-1 and ten cytokines were measured by immunoassays. Vaginal microbiota composition was determined through 16S rRNA amplicon sequencing. MMP-9, TIMP-1 and cytokine concentrations were compared by HIV status, cART, and prematurity and in PWLWH correlations with polymorphonuclear leucocytes, cytokines and bacterial genera were explored.ResultsCVF was available for 50 PWLWH (108 samples) and 12 HUPW (20 samples) between gestation weeks 14-38. Thirty-six PWLWH conceived on cART and 14 initiated post-conception. There were five and one PTB outcomes in PWLWH and HUPW respectively. PWLWH had higher mean CVF concentrations of MMP-9 (p<0.001) and TIMP-1 (p=0.035) in the second trimester compared with HUPW with a similar trend in the third trimester. PWLWH also had higher CVF values of cytokines: IL-1β, IL-8, IL-12 and TNF-α in both trimesters compared to HUPW (p ≤ 0.003). In PWLWH, MMP-9 positively correlated with TIMP-1 (r=0.31, p=0.002) and CVF polymorphonuclear leucocytes (r=0.57, p=0.02). Correlations were observed between MMP-9 and three cytokines: IL-1β (r=0.61), IL-8 (r=0.57) and TNF-α (r=0.64), p<0.001, similarly for TIMP-1. Abundance of anaerobic pathobionts correlated with MMP-9: Gardnerella (r=0.44, p<0.001), Atopobium (r=0.33, p=0.005), and Prevotella genera (r=0.39, p<0.001). Conversely proportion of Lactobacillus genera negatively correlated with MMP-9 (rho=-0.46, p<0.001). MMP-9/TIMP-1 ratio increased with gestational age at sampling in PWLWH, but this was no longer significant after adjusting for confounders and no difference by prematurity was observed in this sub-study.ConclusionsHere we show strong correlations of MMP-9 to genital tract inflammation and sub-optimal bacterial genera in PWLWH indicating the ascending genital tract infection pathway may be a contributory mechanism to the high risk of PTB.

Identification of Novel Protein Biomarkers of Preterm Birth in Human Cervical−Vaginal Fluid

2007 ◽  
Vol 6 (4) ◽  
pp. 1269-1276 ◽  
Author(s):  
Leonardo Pereira ◽  
Ashok P. Reddy ◽  
Thomas Jacob ◽  
Archana Thomas ◽  
Kimberly A. Schneider ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Vaginal Fluid ◽  
Protein Biomarkers ◽  
Novel Protein

Bacterial Vaginosis, Vaginal Fluid Neutrophil Defensins, and Preterm Birth

2003 ◽  
Vol 101 (5, Part 1) ◽  
pp. 862-868
Author(s):  
Rukmini B. Balu ◽  
David A. Savitz ◽  
Cande V. Ananth ◽  
Katherine E. Hartmann ◽  
William C. Miller ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Bacterial Vaginosis ◽  
Vaginal Fluid

scholarly journals Determination of pH as a method of prevention of preterm birth of infectious genesis in women with a history of miscarrage

2015 ◽  
Vol 64 (1) ◽  
pp. 29-33
Author(s):  
Tamara Aleksandrovna Pluzhnikova
Keyword(s):  
Preterm Birth ◽  
Ph Control ◽  
Control Group ◽  
Vaginal Fluid ◽  
Urogenital Infection ◽  
Vaginal Ph ◽  
Fold Reduction ◽  
History Of ◽  
Group 2

There is substantial evidence that urogenital infection is one of the major causes of preterm birth, with bacterial vaginosis being one of these infections. Timely diagnosis and treatment of this condition significantly reduce the rate of preterm birth. The aim of this study was to evaluate the method of pH determination of vaginal fluid in women at a risk of preterm birth. A total of 66 women were included in the study. The women were divided into two groups. Group 1 consisted of 30 women, who measured vaginal pH from week 12 and 36 of gestation twice a week using the test рН-Balance (PremiumDiagnostics, Germany). Group 2 comprised 36 women, who did not measure vaginal pH (control group). Abnormal рН (>4,4) were observed in 9 women (30.0 %). Of them, in 8 women a urogenital infection was observed. All patients received etiotropic treatment. pH measuring in women with a history of miscarriage resulted in 4.5-fold reduction of preterm birth (from 30.0 to 6.7 %). In the control group the was no significant reduction in the rate of preterm birth (from 22.2 to 19.4 %).

200 Bacterial vaginosis, vaginal fluid defensins and preterm birth in a cohort of North Carolina women

2001 ◽  
Vol 185 (6) ◽  
pp. S136
Author(s):  
Rukmini Balu ◽  
David Savitz ◽  
Cande Ananth ◽  
John Thorp ◽  
Philip Heine ◽  
...  
Keyword(s):  
North Carolina ◽  
Preterm Birth ◽  
Bacterial Vaginosis ◽  
Vaginal Fluid

Non-invasive diagnosis of intra-amniotic infection and preterm birth from proteomic analysis of vaginal fluid

2006 ◽  
Vol 195 (6) ◽  
pp. S5 ◽  
Author(s):  
Jane Hitti ◽  
Jodi Lapidus ◽  
Xinfang Lu ◽  
Leonardo Pereira ◽  
David Eschenbach ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Proteomic Analysis ◽  
Vaginal Fluid ◽  
Non Invasive
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