scholarly journals Identification and Characterization of Disulfide Bonds in Proteins and Peptides from Tandem MS Data by Use of the MassMatrix MS/MS Search Engine

2008 ◽  
Vol 7 (1) ◽  
pp. 138-144 ◽  
Author(s):  
Hua Xu ◽  
Liwen Zhang ◽  
Michael A. Freitas
Keyword(s):  
Search Engine ◽  
Disulfide Bonds ◽  
Tandem Ms ◽  
Identification And Characterization ◽  
Ms Search ◽  
Proteins And Peptides

scholarly journals Isolation and characterization of protoporphyrin glycoconjugates from rat Harderian gland by HPLC, capillary electrophoresis and HPLC/electrospray ionization MS

2000 ◽  
Vol 347 (3) ◽  
pp. 757-761 ◽  
Author(s):  
Chang Kee LIM ◽  
M. Aaqib RAZZAQUE ◽  
Jinli LUO ◽  
Peter B. FARMER
Keyword(s):  
Capillary Electrophoresis ◽  
Electrospray Ionization ◽  
Harderian Gland ◽  
Tandem Ms ◽  
Isolation And Characterization ◽  
Harderian Glands ◽  
On Line ◽  
Identification And Characterization

It has been widely reported that the Harderian gland, present in most vertebrates, accumulates high levels of porphyrins, particularly protoporphyrin. The present study describes the extraction, identification and characterization of a group of hitherto unreported protoporphyrin glycoconjugates in the rat Harderian gland using HPLC, capillary electrophoresis, on-line HPLC/electrospray ionization MS and tandem MS. The major glycoconjugate was identified as protoporphyrin-1-O-acyl β-xyloside with a smaller amount of protoporphyrin-1-O-acyl β-glucoside also detected. In the Harderian glands studied, 50-70% of the porphyrins present were in the form of protoporphyrin glycoconjugates. This is the first reported occurrence of glycoconjugates of porphyrins in Nature and suggests that previous studies have wrongly identified the major porphyrin in the Harderian gland as the unconjugated protoporphyrin.

scholarly journals Tandem MS and C60 SIMS for the identification and characterization of lipids

2014 ◽  
Vol 46 (S1) ◽  
pp. 118-122 ◽  
Author(s):  
Anita Durairaj ◽  
Nicholas Winograd
Keyword(s):  
Tandem Ms ◽  
Identification And Characterization

scholarly journals Identification and Characterization of an OSH1 Thiol Reductase from Populus trichocarpa

Cells ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 76
Author(s):  
Wei ◽  
Zhou ◽  
Xu ◽  
Movahedi ◽  
Sun ◽  
...  
Keyword(s):  
Disulfide Bonds ◽  
Populus Trichocarpa ◽  
Expression System ◽  
Homologous Gene ◽  
Cd Stress ◽  
Ros Scavenging ◽  
Prokaryotic Expression System ◽  
Thiol Reductase ◽  
Identification And Characterization

Interferon gamma-induced lysosomal thiol reductase (GILT) is abundantly expressed in antigen-presenting cells and participates in the treatment and presentation of antigens by major histocompatibility complex II. Also, GILT catalyzes the reduction of disulfide bonds, which plays an important role in cellular immunity. (1) Background: At present, the studies of GILT have mainly focused on animals. In plants, GILT homologous gene (Arabidopsis thaliana OSH1: AtOSH1) was discovered in the forward screen of mutants with compromised responses to sulphur nutrition. However, the complete properties and functions of poplar OSH1 are unclear. In addition, CdCl2 stress is swiftly engulfing the limited land resources on which humans depend, restricting agricultural production. (2) Methods: A prokaryotic expression system was used to produce recombinant PtOSH1 protein, and Western blotting was performed to identify its activity. In addition, a simplified version of the floral-dip method was used to transform A. thaliana. (3) Results: Here, we describe the identification and characterization of OSH1 from Populus trichocarpa. The deduced PtOSH1 sequence contained CQHGX2ECX2NX4C and CXXC motifs. The transcript level of PtOSH1 was increased by cadmium (Cd) treatment. In addition, recombinant PtOSH1 reduced disulfide bonds. A stress assay showed that PtOSH1-overexpressing (OE) A. thaliana lines had greater resistance to Cd than wild-type (WT) plants. Also, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in PtOSH1-OE plants were significantly higher than those in WT A. thaliana. These results indicate that PtOSH1 likely plays an important role in the response to Cd by regulating the reactive oxygen species (ROS)-scavenging system. (4) Conclusions: PtOSH1 catalyzes the reduction of disulfide bonds and behaves as a sulfhydryl reductase under acidic conditions. The overexpression of PtOSH1 in A. thaliana promoted root development, fresh weight, and dry weight; upregulated the expression levels of ROS scavenging-related genes; and improved the activity of antioxidant enzymes, enhancing plant tolerance to cadmium (Cd) stress. This study aimed to provide guidance that will facilitate future studies of the function of PtOSH1 in the response of plants to Cd stress.

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