scholarly journals Optimization of MALDI-TOF MS Detection for Enhanced Sensitivity of Affinity-Captured Proteins Spanning a 100 kDa Mass Range

2007 ◽  
Vol 6 (11) ◽  
pp. 4517-4524 ◽  
Author(s):  
Christine L. Gatlin-Bunai ◽  
Lisa H. Cazares ◽  
William E. Cooke ◽  
Oliver J. Semmes ◽  
Dariya I. Malyarenko
Keyword(s):  
Mass Range ◽  
Maldi Tof Ms ◽  
Ms Detection ◽  
Maldi Tof ◽  
Enhanced Sensitivity ◽  
Tof Ms

Nanoporous silica coupled MALDI-TOF MS detection of Bence-Jones proteins in human urine for diagnosis of multiple myeloma

Talanta ◽  
2019 ◽  
Vol 200 ◽  
pp. 288-292 ◽  
Author(s):  
Shuping Long ◽  
Qin Qin ◽  
Yuning Wang ◽  
Yi Yang ◽  
Yan Wang ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Human Urine ◽  
Nanoporous Silica ◽  
Maldi Tof Ms ◽  
Ms Detection ◽  
Maldi Tof ◽  
Tof Ms ◽  
Bence Jones Proteins

Core–Shell Gold Nanoparticles as Non-Conventional Matrix for the MALDI-ToF-MS Detection of Amino Acids: A Preliminary Study

2008 ◽  
Vol 6 (4) ◽  
pp. 654-661 ◽  
Author(s):  
N. Cioffi ◽  
F. De Palo ◽  
C. D. Calvano ◽  
I. D. van der Werf ◽  
F. Palmisano ◽  
...  
Keyword(s):  
Amino Acids ◽  
Gold Nanoparticles ◽  
Core Shell ◽  
Maldi Tof Ms ◽  
Ms Detection ◽  
Maldi Tof ◽  
Preliminary Study ◽  
Tof Ms

scholarly journals Optimizing Thiophosphorylation in the Presence of Competing Phosphorylation with MALDI-TOF−MS Detection

2005 ◽  
Vol 4 (5) ◽  
pp. 1863-1866 ◽  
Author(s):  
Laurie L. Parker ◽  
Alexander B. Schilling ◽  
Stephen J. Kron ◽  
Stephen B. H. Kent
Keyword(s):  
Maldi Tof Ms ◽  
Ms Detection ◽  
Maldi Tof ◽  
Tof Ms

Comparison of imipenem and meropenem antibiotics for the MALDI-TOF MS detection of carbapenemase activity

2017 ◽  
Vol 137 ◽  
pp. 30-33 ◽  
Author(s):  
Veronika Rotova ◽  
Costas C. Papagiannitsis ◽  
Anna Skalova ◽  
Katerina Chudejova ◽  
Jaroslav Hrabak
Keyword(s):  
Maldi Tof Ms ◽  
Ms Detection ◽  
Maldi Tof ◽  
Tof Ms

MALDI-TOF MS detection of dilute, volume-limited peptide samples with physiological salt levels

The Analyst ◽  
2004 ◽  
Vol 129 (9) ◽  
pp. 817 ◽  
Author(s):  
Xiaoyan Zhao ◽  
Jennifer Barber-Singh ◽  
Scott A. Shippy
Keyword(s):  
Maldi Tof Ms ◽  
Ms Detection ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Rapid MALDI-TOF-MS Detection of Anthocyanins in Wine and Grape Using Different Matrices

2010 ◽  
Vol 4 (1) ◽  
pp. 108-115 ◽  
Author(s):  
Violeta Ivanova ◽  
Ágnes Dörnyei ◽  
Marina Stefova ◽  
Trajće Stafilov ◽  
Borimir Vojnoski ◽  
...  
Keyword(s):  
Maldi Tof Ms ◽  
Ms Detection ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Standardized Approach to Proteome Profiling of Human Serum Based on Magnetic Bead Separation and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

2005 ◽  
Vol 51 (6) ◽  
pp. 973-980 ◽  
Author(s):  
Sven Baumann ◽  
Uta Ceglarek ◽  
Georg Martin Fiedler ◽  
Jan Lembcke ◽  
Alexander Leichtle ◽  
...  
Keyword(s):  
Human Serum ◽  
Mass Range ◽  
Magnetic Bead ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
And Storage ◽  
Magnetic Bead Separation ◽  
Tof Ms

Abstract Background: Magnetic bead purification for the analysis of low-abundance proteins in body fluids facilitates the identification of potential new biomarkers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The aims of our study were to establish a proteome fractionation technique and to validate a standardized blood sampling, processing, and storage procedure for proteomic pattern analysis. Methods: We used magnetic bead separation for proteome profiling of human blood by MALDI-TOF MS (mass range, 1000–10 000 Da) and studied the effects on the quality and reproducibility of the proteome analysis of anticoagulants, blood clotting, time and temperature of sample storage, and the number of freeze–thaw cycles of samples. Results: The proteome pattern of human serum was characterized by ∼350 signals in the mass range of 1000–10 000 Da. The proteome profile showed time-dependent dynamic changes before and after centrifugation of the blood samples. Serum mass patterns differed between native samples and samples frozen once. The best reproducibility of proteomic patterns was with a single thawing of frozen serum samples. Conclusion: Application of the standardized preanalytical blood sampling and storage procedure in combination with magnetic bead-based fractionation decreases variability of proteome patterns in human serum assessed by MALDI-TOF MS.

In situ derivatization of Au nanoclusters via aurophilic interactions of a triphenylphosphine gold(i) salt with neurotransmitters and their rapid MALDI-TOF-MS detection in mice brain tissue extracts

2020 ◽  
Vol 8 (1) ◽  
pp. 38-44 ◽  
Author(s):  
Sivakumar Palanisamy ◽  
Shuai Huang ◽  
Huiyuan Zhao ◽  
Di Zhu ◽  
Xiaozhe Zhang
Keyword(s):  
Brain Tissue ◽  
Au Nanoclusters ◽  
Maldi Tof Ms ◽  
Ms Detection ◽  
Maldi Tof ◽  
Tissue Extracts ◽  
Mice Brain ◽  
Aurophilic Interactions ◽  
Tof Ms

Rapid MALDI-TOF-MS detection of neurotransmitters in mice brain tissue extracts using triphenylphosphine gold(i) salt as an efficient matrix.

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