scholarly journals Head-to-Head Comparison of Serum Fractionation Techniques

2007 ◽  
Vol 6 (2) ◽  
pp. 828-836 ◽  
Author(s):  
Jeffrey R. Whiteaker ◽  
Heidi Zhang ◽  
Jimmy K. Eng ◽  
Ruihua Fang ◽  
Brian D. Piening ◽  
...  
Keyword(s):  
Serum Fractionation

INTERFERENCE BY SERUM PROTEINS WITH LH RADIOIMMUNOASSAY USING IMMUNOSORBENT

1973 ◽  
Vol 72 (2) ◽  
pp. 235-242 ◽  
Author(s):  
A. M. Reuter ◽  
J. C. Hendrick ◽  
J. Sulon ◽  
P. Franchimont
Keyword(s):  
Molecular Weight ◽  
Human Serum ◽  
High Molecular Weight ◽  
Serum Proteins ◽  
Void Volume ◽  
High Molecular Weight Proteins ◽  
Serum Fractionation ◽  
Covalently Bound

ABSTRACT The percentage of LH* bound to antibodies that have been covalently bound to cellulose is diminished in the presence of LH-free human serum and sera from various species of animals. Serum fractionation studies on Sephadex G 200 show that the greatest interference comes from the proteins eluted in the void volume i. e. the high molecular weight proteins. Specifically, the gamma M globulins and the α2-macroglobulins appear to play an important role, as demonstrated by tests in which these proteins were neutralized by gamma M and α2-macroglobulin antisera.

Effect of donor bovine serum fractionation on preimplantation mouse embryos in culture

1994 ◽  
Vol 41 (1) ◽  
pp. 257 ◽  
Author(s):  
C.N. Millham ◽  
M.B. Tornesi ◽  
A.T. Palasz ◽  
J. Archer
Keyword(s):  
Bovine Serum ◽  
Mouse Embryos ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
Serum Fractionation

NEPHROTIC SYNDROME IN EARLY INFANCY AS A MANIFESTATION OF CONGENITAL SYPHILIS

PEDIATRICS ◽  
1961 ◽  
Vol 27 (4) ◽  
pp. 636-641
Author(s):  
Angelos C. Papaioannou ◽  
Gertrude G. Asrow ◽  
Natalie H. Schuckmell
Keyword(s):  
Nephrotic Syndrome ◽  
Early Diagnosis ◽  
Renal Disease ◽  
Congenital Syphilis ◽  
Venereal Disease ◽  
Clinical Manifestations ◽  
Paper Electrophoresis ◽  
Early Infancy ◽  
Etiologic Factor ◽  
Serum Fractionation

The case of a 10-week-old infant with a nephrotic syndrome due to congenital syphilis is presented. Negative prenatal history for venereal disease may be misleading. Therefore, a renewed emphasis of congenital syphilis as an etiologic factor of the nephrotic syndrome in early infancy is proposed. Protein in serum fractionation by paper electrophoresis may be of assistance in the differentiation of a primary or secondary renal disease. Early diagnosis of congenital syphilis as a cause of the nephrotic syndrome and specific antisyphilitic therapy will result in prompt remission of the clinical manifestations of this disease.

Acquired von Willebrand’s Syndrome in the Course of Waldenström’s Disease

1980 ◽  
Vol 44 (03) ◽  
pp. 115-118 ◽  
Author(s):  
Claudine Mazurier ◽  
Armelle Parquet-Gernez ◽  
Jacques Descamps ◽  
Francis Bauters ◽  
Maurice Goudemand
Keyword(s):  
Regressive Evolution ◽  
Cofactor Activity ◽  
Auto Antibody ◽  
Ristocetin Cofactor ◽  
Serum Fractionation ◽  
Ristocetin Cofactor Activity

SummaryAcquired von Willebrand's syndrome with a regressive evolution is described in a 66 year old man with Waldenström's disease. An inhibitor electively directed against Ristocetin cofactor activity has been demonstrated, active in vitro after incubation at 37° C. Serum fractionation showed that the inhibitor was independant of the monoclonal IgM and subsequent purification that it was IgG in nature. The results permit its classification as an auto-antibody.

scholarly journals SERUM FRACTIONATION AND THE EFFECTS OF BOVINE SERUM FRACTIONS ON HUMAN CELLS GROWN IN A CHEMICALLY DEFINED MEDIUM

1961 ◽  
Vol 10 (3) ◽  
pp. 389-401 ◽  
Author(s):  
Richard Holmes ◽  
Sonya W. Wolfe
Keyword(s):  
Sodium Bicarbonate ◽  
Hela Cells ◽  
Bovine Serum ◽  
Defined Medium ◽  
Human Cells ◽  
Cell Multiplication ◽  
Chemically Defined Medium ◽  
Continuous Growth ◽  
Serum Fractions ◽  
Serum Fractionation

Serum has been fractionated by curtain electrophoresis using carboxymethyl cellulose dissolved in sodium bicarbonate electrolyte. Various fractions were produced from bovine serum and added to replicate cultures of Chang's endoepithelial cells and HeLa cells grown in a chemically defined medium. The effects of each of the various fractions on the appearance of the cultures and on cell multiplication were studied. Three different fractions were obtained and two were subjected to further purification. One fraction associated with albumin promoted survival, attachment, and flattening as well as cell multiplication. A second fraction associated with the alpha globulins promoted survival and multiplication of some cells. A third fraction caused cells to aggregate and form free floating clumps. An adequate chemically defined medium for continuous growth of human cells was used throughout the study. The response of cells to alterations in their environment which simulated some of the effects produced by serum fractions is described.

Serum fractionation on immobilized pH gradients with one- and two-dimensional techniques

1984 ◽  
Vol 5 (4) ◽  
pp. 209-216 ◽  
Author(s):  
Elisabetta Gianazza ◽  
Adele Frigerio ◽  
Anna Tagliabue ◽  
Pier Giorgio Righetti
Keyword(s):  
Two Dimensional ◽  
Ph Gradients ◽  
Serum Fractionation

scholarly journals Comparison of serum fractionation methods by data independent label-free proteomics

2015 ◽  
Vol 9 ◽  
pp. 14-22 ◽  
Author(s):  
D. Baiwir ◽  
G. Mazzucchelli ◽  
N. Smargiasso ◽  
F. Quesada-Calvo ◽  
E. De Pauw ◽  
...  
Keyword(s):  
Label Free ◽  
Serum Fractionation

scholarly journals Nodular Glomerulosclerosis with Deposition of Monoclonal Immunoglobulin Heavy Chains Lacking CH1

1999 ◽  
Vol 10 (3) ◽  
pp. 519-528
Author(s):  
BRUNO MOULIN ◽  
SOPHIE DERET ◽  
XAVIER MARIETTE ◽  
OLIVIER KOURILSKY ◽  
HIROKAZU IMAI ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Plasma Cells ◽  
Apparent Molecular Weight ◽  
Light Chains ◽  
Monoclonal Immunoglobulin ◽  
Heavy Chains ◽  
Nodular Glomerulosclerosis ◽  
Heavy Chain Deposition Disease ◽  
Serum Fractionation

Abstract. The objective of this study was to further characterize the clinical and immunopathologic features of heavy chain deposition disease (HCDD), a recently described entity. Four patients were diagnosed as having HCDD on a kidney biopsy. All presented with nodular glomerulosclerosis with deposition of γ1 heavy chains lacking CH1 epitopes, but without light chains. Two different patterns were observed in the serum. First, patients 1 and 2 had a circulating monoclonal IgGλ containing a short γ1 heavy chain lacking CH1 epitopes, with an apparent molecular weight of 40 kD consistent with a complete CH1 deletion. Biosynthetic experiments also showed that the deleted heavy chain was produced in excess compared with light chains, and was secreted in vitro together with half Ig molecules, although these abnormal components were not detected by Western blot analysis of whole serum. Second, patients 3 and 4 had a circulating monoclonal IgG1λ with an apparently normal, nondeleted heavy chain subunit, but serum fractionation followed by immunoblotting revealed an isolated monoclonal γ1 chain lacking CH1 epitopes. These data strongly suggest that renal deposition of a CH1-deleted heavy chain circulating in low amounts in the serum as a free unassembled subunit is a major feature of HCDD. The CH1 deletion is most likely responsible for the premature secretion in blood of the heavy chain by a clone of plasma cells.

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