Molecular Profiling of Experimental Parkinson's Disease:  Direct Analysis of Peptides and Proteins on Brain Tissue Sections by MALDI Mass Spectrometry

2004 ◽  
Vol 3 (2) ◽  
pp. 289-295 ◽  
Author(s):  
Johan Pierson ◽  
Jeremy L. Norris ◽  
Hans-Rudolf Aerni ◽  
Per Svenningsson ◽  
Richard M. Caprioli ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Brain Tissue ◽  
Direct Analysis ◽  
Maldi Mass Spectrometry ◽  
Molecular Profiling ◽  
Tissue Sections

Rapid Diagnosis of Parkinson’s Disease from Sebum using Paper Spray Ionisation Ion Mobility Mass Spectrometry

2020 ◽  
Author(s):  
Depanjan Sarkar ◽  
Drupad Trivedi ◽  
Eleanor Sinclair ◽  
Sze Hway Lim ◽  
Caitlin Walton-Doyle ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Ion Mobility ◽  
Neurodegenerative Disorder ◽  
Treatment Options ◽  
Ion Mobility Mass Spectrometry ◽  
Paper Spray ◽  
Molecular Features ◽  
Validation Set

Parkinson’s disease (PD) is the second most common neurodegenerative disorder for which identification of robust biomarkers to complement clinical PD diagnosis would accelerate treatment options and help to stratify disease progression. Here we demonstrate the use of paper spray ionisation coupled with ion mobility mass spectrometry (PSI IM-MS) to determine diagnostic molecular features of PD in sebum. PSI IM-MS was performed directly from skin swabs, collected from 34 people with PD and 30 matched control subjects as a training set and a further 91 samples from 5 different collection sites as a validation set. PSI IM-MS elucidates ~ 4200 features from each individual and we report two classes of lipids (namely phosphatidylcholine and cardiolipin) that differ significantly in the sebum of people with PD. Putative metabolite annotations are obtained using tandem mass spectrometry experiments combined with accurate mass measurements. Sample preparation and PSI IM-MS analysis and diagnosis can be performed ~5 minutes per sample offering a new route to for rapid and inexpensive confirmatory diagnosis of this disease.

scholarly journals Sepiapterin reductase expression is increased in Parkinson's disease brain tissue

2007 ◽  
Vol 1139 ◽  
pp. 42-47 ◽  
Author(s):  
Jennifer E. Tobin ◽  
Jing Cui ◽  
Jemma B. Wilk ◽  
Jeanne C. Latourelle ◽  
Jason M. Laramie ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Brain Tissue ◽  
Sepiapterin Reductase

New Developments in Profiling and Imaging of Proteins from Tissue Sections by MALDI Mass Spectrometry

2006 ◽  
Vol 5 (11) ◽  
pp. 2889-2900 ◽  
Author(s):  
Pierre Chaurand ◽  
Jeremy L. Norris ◽  
D. Shannon Cornett ◽  
James A. Mobley ◽  
Richard M. Caprioli
Keyword(s):  
Mass Spectrometry ◽  
Maldi Mass Spectrometry ◽  
New Developments ◽  
Tissue Sections

scholarly journals Phosphorylated Alpha-Synuclein Within Cutaneous Autonomic Nerves of Patients With Parkinson’s Disease: The Implications of Sample Thickness on Results

2020 ◽  
Vol 68 (10) ◽  
pp. 669-678
Author(s):  
Ningshan Wang ◽  
Jennifer Garcia ◽  
Roy Freeman ◽  
Christopher H. Gibbons
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Alpha Synuclein ◽  
Sweat Glands ◽  
Autonomic Nerves ◽  
Pgp 9.5 ◽  
Protein Gene Product ◽  
Tissue Sections ◽  
Increased Sensitivity ◽  
Skin Biopsies

The detection of cutaneous phosphorylated alpha-synuclein (P-syn) in patients with Parkinson’s disease (PD) has ranged from 30% to 100% across different studies. We hypothesize that part of the variability in P-syn detection is due to methodological differences using sections of different tissue thickness. Three skin biopsies were obtained from 29 individuals with PD and 21 controls. Tissues were cut into 10-, 20-, and 50-µm-thick sections and double-stained with protein gene product (PGP) 9.5 and P-syn. We quantified the deposition of P-syn with and without PGP 9.5 in sweat glands, pilomotor muscle, and blood vessels using confocal digital images of autonomic structures. Overall, the P-syn-positive rates with PGP 9.5 colocalization in subjects with PD were 100% using 50 µm sections, 90% using 20 µm sections, and 73% using 10 µm sections with 100% specificity. (No P-syn was detected within control subjects.) Without PGP 9.5, colocalization of the P-syn-positive rates was 100% for all samples, but specificity dropped below 70%. In this study, double-immunostained 50 µm skin biopsy tissue sections are superior to 20 and 10 µm tissue sections at detecting P-syn in subjects with PD. The increased sensitivity is likely secondary to a combination of greater volume of tissue analyzed and improved visualization of nerve fiber architecture.

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