Characterization of theSalmonellaTyphimurium Proteome by Semi-automated Two Dimensional HPLC-Mass Spectrometry: Detection of Proteins Implicated in Multiple Antibiotic Resistance

2004 ◽  
Vol 3 (3) ◽  
pp. 595-603 ◽  
Author(s):  
Nick G. Coldham ◽  
Martin J. Woodward
Keyword(s):  
Mass Spectrometry ◽  
Antibiotic Resistance ◽  
Two Dimensional ◽  
Multiple Antibiotic Resistance ◽  
Mass Spectrometry Detection

Characterization of the polyphenolic fraction of pomegranate samples by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry detection

2019 ◽  
Vol 34 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Marina Russo ◽  
Francesco Cacciola ◽  
Katia Arena ◽  
Domenica Mangraviti ◽  
Laura de Gara ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Two Dimensional ◽  
Mass Spectrometry Detection

Pyrolysis of Residual Tobacco Seeds: Characterization of Nitrogen Compounds in Bio-oil Using Comprehensive Two-Dimensional Gas Chromatography with Mass Spectrometry Detection

2017 ◽  
Vol 31 (9) ◽  
pp. 9402-9407 ◽  
Author(s):  
Bruna Onorevoli ◽  
Maria E. Machado ◽  
Allan dos S. Polidoro ◽  
Valeriano A. Corbelini ◽  
Elina B. Caramão ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Nitrogen Compounds ◽  
Two Dimensional ◽  
Mass Spectrometry Detection ◽  
Tobacco Seeds ◽  
Bio Oil

scholarly journals Incidence and Characterization of Integrons, Genetic Elements Mediating Multiple-Drug Resistance, in AvianEscherichia coli

1999 ◽  
Vol 43 (12) ◽  
pp. 2925-2929 ◽  
Author(s):  
Lydia Bass ◽  
Cynthia A. Liebert ◽  
Margie D. Lee ◽  
Anne O. Summers ◽  
David G. White ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Multiple Drug Resistance ◽  
Marker Gene ◽  
Clinical Isolates ◽  
Multiple Antibiotic Resistance ◽  
Class 1 Integron ◽  
E Coli ◽  
Class 1

ABSTRACT Antibiotic resistance among avian bacterial isolates is common and is of great concern to the poultry industry. Approximately 36% (n = 100) of avian, pathogenic Escherichia coli isolates obtained from diseased poultry exhibited multiple-antibiotic resistance to tetracycline, oxytetracycline, streptomycin, sulfonamides, and gentamicin. Clinical avian E. coli isolates were further screened for the presence of markers for class 1 integrons, the integron recombinase intI1 and the quaternary ammonium resistance gene qacEΔ1, in order to determine the contribution of integrons to the observed multiple-antibiotic resistance phenotypes. Sixty-three percent of the clinical isolates were positive for the class 1 integron markersintI1 and qacEΔ1. PCR analysis with the conserved class 1 integron primers yielded amplicons of approximately 1 kb from E. coli isolates positive for intI1 andqacEΔ1. These PCR amplicons contained the spectinomycin-streptomycin resistance gene aadA1. Further characterization of the identified integrons revealed that many were part of the transposon Tn21, a genetic element that encodes both antibiotic resistance and heavy-metal resistance to mercuric compounds. Fifty percent of the clinical isolates positive for the integron marker gene intI1 as well as for theqacEΔ1 and aadA1 cassettes also contained the mercury reductase gene merA. The correlation between the presence of the merA gene with that of the integrase and antibiotic resistance genes suggests that these integrons are located in Tn21. The presence of these elements among avianE. coli isolates of diverse genetic makeup as well as inSalmonella suggests the mobility of Tn21 among pathogens in humans as well as poultry.

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