Combining Metabolic Pathway Design and Retrosynthetic Planning for the Design of a Novel Semisynthetic Manufacturing Scheme for Paclitaxel

2014 ◽  
Vol 18 (6) ◽  
pp. 816-826 ◽  
Author(s):  
Vikramaditya G. Yadav
Keyword(s):  
Metabolic Pathway ◽  
Metabolic Pathway Design

scholarly journals A retrosynthetic biology approach to metabolic pathway design for therapeutic production

2011 ◽  
Vol 5 (1) ◽  
pp. 122 ◽  
Author(s):  
Pablo Carbonell ◽  
Anne-Gaëlle Planson ◽  
Davide Fichera ◽  
Jean-Loup Faulon
Keyword(s):  
Metabolic Pathway ◽  
Metabolic Pathway Design

Innovative metabolic pathway design for efficient l-glutamate production by suppressing CO2 emission

2007 ◽  
Vol 103 (3) ◽  
pp. 262-269 ◽  
Author(s):  
Akito Chinen ◽  
Yuri I. Kozlov ◽  
Yoshihiko Hara ◽  
Hiroshi Izui ◽  
Hisashi Yasueda
Keyword(s):  
Metabolic Pathway ◽  
Co2 Emission ◽  
Glutamate Production ◽  
Metabolic Pathway Design

PERLAKUAN ETHYL METHANE SULFONATE (EMS) PADA ENTEROBACTER AEROGENES AY-2 DARI LIMBAH METAN FERMENTASI UNTUK PENINGKATAN PRODUKSI GAS HIDROGEN

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Mahyudin Abdul Rachman
Keyword(s):  
Metabolic Pathway ◽  
Acid Production ◽  
Double Mutant ◽  
Enterobacter Aerogenes ◽  
H2 Production ◽  
Hydrogen Gas ◽  
Survival Ratio ◽  
Ethyl Methane Sulfonate ◽  
Methane Sulfonate ◽  
Ethyl Methane

Enterobacter aerogenes AY-2 mutant is known for hydrogen gas producer which ws obtained from the sludge of methane fermentation and the yield is 1.5 fold higher than wildtype. Hydrogen gas production can be gain via NADH oxidation in anaerobic metabolic pathway by blocking organic acid production. Metabolic pathway can be changed by mutagenesis. Enterobacter aerogenes AY-2 mutated with ethyl methane sulfonate in logarithmic phase with consentration 10, 11, 12, 13, 14 and 15 μl/ml cell suspention during 120 minute. Mutation that result lowest survival ratio (0,01%) was 14 μl EMS/ml cell suspention is repeated with variation incubation time, 30, 60, 90 and 120 minute. 166 double mutant colony has been collected and choosen randomly. The choosen 43 colony was fermented in glycerol complex medium for determining ten double mutant with the highest H2 production. Double mutant AD-H43 is a highest H2 producer that increase 20% H2 production from AY-2 and has a decrease lactid acid production, 31% less from AY-2. Increasing H2 production in double mutant AD-H43 is caused by lactate dehydrogenase deffi cient.Keywords: Enterobacter aerogenes AY-2, ethyl methane sulfonate (EMS), H2 and methane sludge

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