Improved Continuous Flow Processing: Benzimidazole Ring Formation via Catalytic Hydrogenation of an Aromatic Nitro Compound

2013 ◽  
Vol 18 (11) ◽  
pp. 1427-1433 ◽  
Author(s):  
Jian Chen ◽  
Katrin Przyuski ◽  
Renee Roemmele ◽  
Roger P. Bakale
Keyword(s):  
Nitro Compound ◽  
Catalytic Hydrogenation ◽  
Continuous Flow ◽  
Ring Formation ◽  
Aromatic Nitro Compound ◽  
Benzimidazole Ring ◽  
Aromatic Nitro

scholarly journals Hawk-Seq™ differentiates between various mutations in Salmonella typhimurium TA100 strain caused by exposure to Ames test-positive mutagens

Mutagenesis ◽  
2021 ◽  
Author(s):  
Yuki Otsubo ◽  
Shoji Matsumura ◽  
Naohiro Ikeda ◽  
Osamu Morita
Keyword(s):  
High Resolution ◽  
Salmonella Typhimurium ◽  
Nitro Compound ◽  
Aromatic Amines ◽  
Ames Test ◽  
Alkylating Agents ◽  
Water Soluble ◽  
Base Substitution ◽  
Aromatic Nitro Compound ◽  
Aromatic Nitro

Abstract A precise understanding of differences in genomic mutations according to the mutagenic mechanisms detected in mutagenicity data is required to evaluate the carcinogenicity of environmental mutagens. Recently, we developed a highly accurate genome sequencing method, ‘Hawk-Seq™’, that enables the detection of mutagen-induced genome-wide mutations. However, its applicability to detect various mutagens and identify differences in mutational profiles is not well understood. Thus, we evaluated DNA samples from Salmonella typhimurium TA100 exposed to 11 mutagens including alkylating agents, aldehydes, an aromatic nitro compound, epoxides, aromatic amines, and polycyclic aromatic hydrocarbons (PAHs). We extensively analysed mutagen-induced mutational profiles and their association with the mechanisms of mutagens. Hawk-Seq™ sensitively detected mutations induced by all 11 mutagens, including one that increased the number of revertants by approximately two-fold in the Ames test. Although the sensitivity for less water-soluble mutagens was relatively low, we increased the sensitivity to obtain high-resolution spectra by modifying the exposure protocol. Moreover, two epoxides indicated similar 6-dimensional or 96-dimensional mutational patterns; likewise, three SN1 type alkylating agents indicated similar mutational patterns, suggesting that the mutational patterns are compound category-specific. Meanwhile, an SN2 type alkylating agent exhibited unique mutational patterns compared to those of the SN1 type alkylating agents. Although the mutational patterns induced by aldehydes, the aromatic nitro compound, aromatic amines, and PAHs did not differ substantially from each other, the maximum total base substitution frequencies (MTSFs) were similar among mutagens in the same structural groups. Furthermore, the MTSF was found to be associated with the carcinogenic potency of some direct-acting mutagens. These results indicate that our method can generate high-resolution mutational profiles to identify characteristic features of each mutagen. The detailed mutational data obtained by Hawk-Seq™ can provide useful information regarding mutagenic mechanisms and help identify its association with the carcinogenicity of mutagens without requiring carcinogenicity data.

A Novel One-pot Benzimidazole Ring Formation via a Continuous Flow Selective Reductive Cyclization Method

2018 ◽  
Vol 22 (19) ◽  
pp. 1940-1944
Author(s):  
Balazs Szabo ◽  
Ferenc Faigl ◽  
Janos Eles ◽  
Istvan Greiner
Keyword(s):  
Continuous Flow ◽  
Ring Formation ◽  
Reductive Cyclization ◽  
Benzimidazole Ring ◽  
One Pot

Photopolymerization in the System Oligomer–Aromatic Nitro Compound–Aniline Derivative

2004 ◽  
Vol 38 (2) ◽  
pp. 115-119 ◽  
Author(s):  
S. V. Zelentsov ◽  
S. V. Gusev ◽  
N. V. Zelentsova ◽  
M. V. Kuznetsov
Keyword(s):  
Nitro Compound ◽  
Aromatic Nitro Compound ◽  
Aniline Derivative ◽  
Aromatic Nitro

Genotoxicity assessment of the antiepileptic drug AMP397, an Ames-positive aromatic nitro compound

2002 ◽  
Vol 518 (2) ◽  
pp. 181-194 ◽  
Author(s):  
Willi Suter ◽  
Andreas Hartmann ◽  
Franziska Poetter ◽  
Peter Sagelsdorff ◽  
Peter Hoffmann ◽  
...  
Keyword(s):  
Antiepileptic Drug ◽  
Nitro Compound ◽  
Aromatic Nitro Compound ◽  
Aromatic Nitro
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