Parameters Controlling the One-Step Derivatization of Controlled Pore Glass with a Diol for Solid-Phase Synthesis of 3‘-Modified Oligonucleotides

2006 ◽  
Vol 10 (3) ◽  
pp. 403-408 ◽  
Author(s):  
Alain Laurent ◽  
Carole Chaix
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Modified Oligonucleotides ◽  
Phase Synthesis ◽  
Controlled Pore Glass ◽  
Pore Glass ◽  
One Step ◽  
The One

A one step derivatization of controlled pore glass for oligonucleotide solid-phase synthesis

2004 ◽  
Vol 45 (48) ◽  
pp. 8883-8887 ◽  
Author(s):  
Alain Laurent ◽  
Bertrand de Lambert ◽  
Marie-Thérèse Charreyre ◽  
Bernard Mandrand ◽  
Carole Chaix
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Phase Synthesis ◽  
Controlled Pore Glass ◽  
Pore Glass ◽  
One Step

Improved Conditions for Solid Phase Synthesis of Oligonucleotides on PS-PEG Copolymers

1995 ◽  
Vol 50 (7) ◽  
pp. 1096-1100 ◽  
Author(s):  
Ernst Bayer ◽  
Konrad Bleicher ◽  
Martin Maier
Keyword(s):  
Electrospray Mass Spectrometry ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Chemical Properties ◽  
Oligonucleotide Synthesis ◽  
Phase Synthesis ◽  
Controlled Pore Glass ◽  
Product Identification ◽  
Pore Glass ◽  
And Chemical Properties

Polystyrene-polyethylene glycol (PS-PEG) tentacle polymers with loadings of up to 60/<μmol/g were used for standard oligonucleotide synthesis. As these resins are easy to handle and stable under reaction and cleavage conditions they may be used alternatively to controlled pore glass (CPG) as the most commonly used solid support for oligonucleotide synthesis. However, structural and chemical properties of the PS-PEG resins require modified conditions to guarantee syntheses with high coupling efficiencies. Oligonucleotides (ODN ) of various sequences and lengths have successfully been synthesized using HPLC and capillary electrophoresis (CE) for purity control. Additionally, electrospray mass spectrometry (ES-MS) was used for product identification.

Oligonucleotide solid-phase synthesis on fluorescent nanoparticles grafted on controlled pore glass

RSC Advances ◽  
2012 ◽  
Vol 2 (31) ◽  
pp. 11858 ◽  
Author(s):  
Gabriel De Crozals ◽  
Carole Farre ◽  
Grégoire Hantier ◽  
Didier Léonard ◽  
Christophe A. Marquette ◽  
...  
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Fluorescent Nanoparticles ◽  
Phase Synthesis ◽  
Controlled Pore Glass ◽  
Pore Glass

Solid Phase Synthesis of Novel Fullerene Nucleotides Conjugates

2011 ◽  
Vol 266 ◽  
pp. 200-203
Author(s):  
Jing Zhang ◽  
Ya Dong Zhang
Keyword(s):  
Aspartic Acid ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Biological Properties ◽  
Azomethine Ylide ◽  
Hydroxyl Group ◽  
Spectrometric Analysis ◽  
Phase Synthesis ◽  
Chemical Structures ◽  
Controlled Pore Glass

N-substituted 3, 4-fullero pyrrolidine was synthesized according to 1, 3-dipolar cycloaddition of the azomethine ylide. Aspartic acid with protected α-amino and α-carboxyl groups was reacted with the activated hydroxyl group of N-substituted 3, 4-fullero pyrrolidine. The products were deprotected, affording the monofullerene aspartic acid (mFas). The conjugate FasT was synthesized by reaction of mFas containing protected amino group with the thymidylic acid derivatived controlled pore glass (CPG) using solid phase synthesis. All of the above fullerene derivatives were characterized by UV–vis, 1H NMR, IR and MS spectrometric analysis, giving the correct spectra with regard to their chemical structure. The chemical structures of fullerene nucleotides conjugate FasT is different from previous reports and may have novel biological properties. Moreover, they are more suitable for applications in biomedical research due to their solubilization in THF and DMSO. They have a potential to be used as monomer for the automatic synthesis. It allows further conjugation with specific biomolecules including amino acids, peptides, nucleotides and nucleic acids. A novel method has been developed to synthesize fullerene nucleotides conjugate. Their unique chemical structures make them very interesting for their potential use in medicine and biology.

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