Double Isocyanide Insertion and C,C-Coupling Reaction of [(C5Me4)SiMe2(N-t-Bu)]ZrMe2. Structural Characterization of the Two 1,4-Diaza-5-zirconacyclopentene Ring Conformations for [(C5Me4)SiMe2(N-t-Bu)]Zr[N(R)C(Me)C(Me)N(R)] Complexes

1997 ◽  
Vol 16 (15) ◽  
pp. 3548-3556 ◽  
Author(s):  
Lioba Kloppenburg ◽  
Jeffrey L. Petersen
Keyword(s):  
Structural Characterization ◽  
Coupling Reaction ◽  
Ring Conformations

The Synthesis and Structural Characterization of Peralkylated Triguanide Superbases

2014 ◽  
Vol 67 (7) ◽  
pp. 1129 ◽  
Author(s):  
Vjekoslav Štrukil ◽  
Edislav Lekšić ◽  
Ernest Meštrović ◽  
Mirjana Eckert-Maksić
Keyword(s):  
Diffraction Analysis ◽  
Gas Phase ◽  
Structural Characterization ◽  
Positive Charge ◽  
Coupling Reaction ◽  
Chloride Salt ◽  
X Ray Diffraction ◽  
X Ray ◽  
First Time

Organic superbases derived from a peralkylated triguanide framework have been synthesized in a coupling reaction between monosubstituted guanidines and a Vilsmeier salt. Single crystal X-ray diffraction analysis of the chloride salt allowed the structural characterization of the benzyl derivative for the first time and revealed an effective delocalization of the positive charge despite significant distortion of the triguanide cation planarity. With the calculated gas phase basicity ranging from 262 to 265 kcal mol–1 and pKa values in acetonitrile between 28 and 30, these compounds have been evaluated as potential organocatalysts in the transesterification reaction of vegetable oil.

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

Structural characterization of the PPIase domain of FKBP51, a cochaperone of human Hsp90

2011 ◽  
Vol 44 (06) ◽  
Author(s):  
A Bracher ◽  
C Kozany ◽  
AK Thost ◽  
F Hausch
Keyword(s):  
Structural Characterization ◽  
Ppiase Domain
Sign in / Sign up

Export Citation Format

Share Document