Building Fluorescent Sensors by Template Polymerization:  The Preparation of a Fluorescent Sensor ford-Fructose

1999 ◽  
Vol 1 (8) ◽  
pp. 1209-1212 ◽  
Author(s):  
Wei Wang ◽  
Shouhai Gao ◽  
Binghe Wang
Keyword(s):  
Fluorescent Sensor ◽  
Fluorescent Sensors ◽  
Template Polymerization

Development of highly selective fluorescent sensors for detection of glycolipids in vesicles and visualization of glutamate for neuronal imaging

2020 ◽  
Author(s):  
◽  
Ming Xu
Keyword(s):  
Fluorescence Enhancement ◽  
Chemical Sensor ◽  
Fluorescent Sensor ◽  
Hydrophobic Effect ◽  
Fluorescent Sensors ◽  
Nucleophilic Aromatic Substitution ◽  
Sensor System ◽  
Aromatic Substitution ◽  
Glutamate Binding ◽  
A Cell

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI-COLUMBIA AT REQUEST OF AUTHOR.] Fluorescent sensors are very useful tools for exploring chemical biology and advanced medical research. Herein, we propose four different fluorescent sensor systems for the recognition of some important biological molecules. The first sensor system is a multi-component fluorescent sensor complex for the sensing of glycolipids. The glycolipid sensor system is a novel design that takes advantage of supramolecular self-assembly. Results show that it can bind with both the sugar headgroup and hydrocarbon tail of glycolipids, and turn on the fluorescence of the sensor system. The second sensor is a cell-impermeable fluorescent sensor system for the recognition and extraction of glycolipids from vesicles. To avoid the fluorescence enhancement caused by the hydrophobic effect from cell membrane, we designed a series of cell-impermeable sensor complexes. In addition, these complexes were fully explored by vesicle studies. Another fluorescent sensor is NS600 which was developed for detecting and imaging glutamate in neurons. This sensor system that utilizes a nucleophilic aromatic substitution for glutamate binding, and produces a 270-fold fluorescence enchantment upon glutamate binding. Also, it overcomes drawbacks of previous glutamate sensors including low signal response and poor sensitivity. It enables a clear and accurate visualization of glutamate in cultural neurons. The last sensor is NS570, a cell-impermeable glutamate sensor which could be loaded into synaptic vesicles by vesicle cycling. This sensor is a reversible chemical sensor that gives a 2600-fold fluorescence enhancement upon the titration with glutamate and can be used to monitor the release of neuronal glutamate in real time.

scholarly journals Which is a better fluorescent sensor: aggregation-induced emission-based nanofibers or thin-coating films?

2020 ◽  
Vol 1 (4) ◽  
pp. 574-578
Author(s):  
Yexin Ding ◽  
Weili Li ◽  
Fangming Wang ◽  
Hongkun Li ◽  
Shengyuan Yang ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
Fluorescent Sensors ◽  
Thin Coating ◽  
Coating Films ◽  
Aggregation Induced Emission

Aggregation-induced emission (AIE)-based fluorescent nanofibers (FNFs) and thin-coating films (FTFs) are employed as visual fluorescent sensors, and their performance dynamics is compared.

Detection of boronic acid derivatives in cells using a fluorescent sensor

2015 ◽  
Vol 13 (25) ◽  
pp. 6927-6930 ◽  
Author(s):  
Yoshihide Hattori ◽  
Miki Ishimura ◽  
Youichirou Ohta ◽  
Hiroshi Takenaka ◽  
Tsubasa Watanabe ◽  
...  
Keyword(s):  
Boronic Acid ◽  
Detection Method ◽  
Fluorescent Sensor ◽  
Fluorescent Sensors ◽  
Chelating Ligands ◽  
Boronic Acid Derivatives ◽  
In Cells

To develop a detection method for boronic acid derivatives, boron-chelating ligands were synthesized as fluorescent sensors for boronic acid derivatives.

scholarly journals Extraction and Application of Natural Rutin From Sophora japonica to Prepare the Novel Fluorescent Sensor for Detection of Copper Ions

2021 ◽  
Vol 9 ◽  
Author(s):  
Shilong Yang ◽  
Lu Sun ◽  
Zhiwen Song ◽  
Li Xu
Keyword(s):  
Inclusion Complex ◽  
Emission Intensity ◽  
Copper Ions ◽  
Fluorescent Sensor ◽  
Fluorescent Sensors ◽  
Emission Peak ◽  
The Novel ◽  
Buffer Solution ◽  
Sophora Japonica ◽  
Fluorescent Emission

Rutin (R), a representative flavonoid found in various biomasses, can be used to prepare different fluorescent sensors for environmental, biological and medical fields. In this work, the natural R in Sophora japonica was extracted and purified to prepare fluorescent-responding sensor systems intended to recognize copper ions with both strong selectivity as well as appropriate sensitivity. Results showed that neat R had no obvious fluorescent emission peak in PBS buffer solution. However, when R and (2-hydroxypropyl)-β-cyclodextrin (CD) were introduced within buffer solution, fluorescent emission intensity was significantly increased due to the resultant R-CD inclusion complex. In addition, the formed R-CD inclusion complex was shown to behave as the aforementioned fluorescent sensor for copper ions through a mechanism of quenched fluorescent emission intensity when R-CD became bound with copper ions. The binding constant value for R-CD with copper ions was 1.33 × 106, allowing for quantification of copper ions between the concentration range of 1.0 × 10–7–4.2 × 10–6mol⋅L–1. Furthermore, the minimum detection limit was found to be 3.5 × 10–8mol⋅L–1. This work showed the prepared R-CD inclusion complex was both highly selective and strongly sensitive toward copper ions, indicating that this system could be applied into various fields where copper ions are of concern.

scholarly journals A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging

Sensors ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 4768 ◽  
Author(s):  
Zongcheng Wang ◽  
Huihuang Zheng ◽  
Chengliang Zhang ◽  
Dongfang Tang ◽  
Qiyao Wu ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
High Sensitivity ◽  
Living Cells ◽  
Fluorescent Sensors ◽  
Quantitative Detection ◽  
Fast Reaction ◽  
Detection Range ◽  
Long Wavelength ◽  
Turn On

The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocysteine fluorescent sensors, we designed and synthesized the compound YZ-A4 as a turn-on fluorescent sensor to detect the content of selenocysteine. The quantitative detection range of the sensor YZ-A4 to selenocysteine was from 0 to 32 μM, and the detection limit was as low as 11.2 nM. The sensor displayed a rapid turn-on response, good selectivity, and high sensitivity to selenocysteine. Finally, we have demonstrated that YZ-A4 could be used for fluorescence imaging of selenocysteine in living cells.

BINOL-linked 1,2,3-triazoles: an unexpected fluorescent sensor with anion–π interaction for iodide ions

RSC Advances ◽  
2014 ◽  
Vol 4 (97) ◽  
pp. 54256-54262 ◽  
Author(s):  
Cai-Yun Wang ◽  
Jin-Feng Zou ◽  
Zhan-Jiang Zheng ◽  
Wei-Sheng Huang ◽  
Li Li ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
Fluorescent Sensors ◽  
Iodide Ions

BINOL-derived triazoles could be used in organocatalytic silylation and unexpectedly as fluorescent sensors for the recognition of I−.

scholarly journals In vitro selection of a sodium-specific DNAzyme and its application in intracellular sensing

2015 ◽  
Vol 112 (19) ◽  
pp. 5903-5908 ◽  
Author(s):  
Seyed-Fakhreddin Torabi ◽  
Peiwen Wu ◽  
Claire E. McGhee ◽  
Lu Chen ◽  
Kevin Hwang ◽  
...  
Keyword(s):  
Metal Ions ◽  
In Vitro Selection ◽  
Fluorescent Sensor ◽  
Critical Role ◽  
Fluorescent Sensors ◽  
Fluorescence Signal ◽  
Divalent Metal Ions ◽  
Intracellular Sensing ◽  
Selection Of

Over the past two decades, enormous progress has been made in designing fluorescent sensors or probes for divalent metal ions. In contrast, the development of fluorescent sensors for monovalent metal ions, such as sodium (Na+), has remained underdeveloped, even though Na+is one the most abundant metal ions in biological systems and plays a critical role in many biological processes. Here, we report the in vitro selection of the first (to our knowledge) Na+-specific, RNA-cleaving deoxyribozyme (DNAzyme) with a fast catalytic rate [observed rate constant (kobs) ∼0.1 min−1], and the transformation of this DNAzyme into a fluorescent sensor for Na+by labeling the enzyme strand with a quencher at the 3′ end, and the DNA substrate strand with a fluorophore and a quencher at the 5′ and 3′ ends, respectively. The presence of Na+catalyzed cleavage of the substrate strand at an internal ribonucleotide adenosine (rA) site, resulting in release of the fluorophore from its quenchers and thus a significant increase in fluorescence signal. The sensor displays a remarkable selectivity (>10,000-fold) for Na+over competing metal ions and has a detection limit of 135 µM (3.1 ppm). Furthermore, we demonstrate that this DNAzyme-based sensor can readily enter cells with the aid of α-helical cationic polypeptides. Finally, by protecting the cleavage site of the Na+-specific DNAzyme with a photolabileo-nitrobenzyl group, we achieved controlled activation of the sensor after DNAzyme delivery into cells. Together, these results demonstrate that such a DNAzyme-based sensor provides a promising platform for detection and quantification of Na+in living cells.

A carbon dot-encapsulated UiO-type metal organic framework as a multifunctional fluorescent sensor for temperature, metal ion and pH detection

2018 ◽  
Vol 6 (16) ◽  
pp. 4396-4399 ◽  
Author(s):  
Chuanxu Yao ◽  
Yan Xu ◽  
Zhiguo Xia
Keyword(s):  
Metal Ion ◽  
Fluorescent Sensor ◽  
Metal Organic Framework ◽  
Fluorescent Sensors ◽  
Carbon Dot ◽  
Metal Organic ◽  
Ph Detection ◽  
Organic Framework

Carbon dot encapsulated UiO-66 composites have been fabricated and designed as multifunctional fluorescent sensors for temperature, pH, and Fe3+ detection.

scholarly journals Ascorbic acid stabilised copper nanoclusters as fluorescent sensors for detection of quercetin

RSC Advances ◽  
2020 ◽  
Vol 10 (15) ◽  
pp. 8989-8993 ◽  
Author(s):  
Zhifeng Cai ◽  
Haoyang Li ◽  
Jinglong Wu ◽  
Li Zhu ◽  
Xinru Ma ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Fluorescent Sensor ◽  
Fluorescent Sensors ◽  
Sensitive Detection ◽  
Copper Nanoclusters

A fluorescent sensor based on ascorbic acid-functionalized copper nanoclusters (AA-Cu NCs) were prepared for the sensitive detection of quercetin.

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